A new bacteria-free strategy induced by MaGal2 facilitates pinewood nematode escape immune response from its vector beetle
作者机构：State Key Laboratory of Integrated Management of Pest Insects and RodentsInstitute of ZoologyChinese Academy of SciencesBeijingChina CAS Center for Excellence in Biotic InteractionsUniversity of Chinese Academy of SciencesBeijingChina
出 版 物：《Insect Science》 (昆虫科学（英文版）)
年 卷 期：2021年第28卷第4期
页 面：1087-1102页
核心收录：
学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 09[农学] 0904[农学-植物保护] 0901[农学-作物学] 0713[理学-生态学]
基 金：This work was funded by the National Natural Science Foundation of China(31630013) Frontier Science Key Project of the Chinese Academy of Sciences(QYZDJ-SSW-SMC024)
主 题：galectin immune evasion pinewood nematode symbiotic bacteria vector beetle
摘 要：Symbiotic microbes play a crucial role in regulating parasite–host interactions;however,the role of bacterial associates in parasite–host interactions requires *** this study,we showed that,instead of introducing numerous symbiotic bacteria,dispersal of 4th-stage juvenile(JIV)pinewood nematodes(PWNs),Bursaphelenchus xylophilus,only introduced few bacteria to its vector beetle,Monochamus alternatus(Ma).JIV showed weak binding ability to five dominant bacteria species isolated from the beetles’pupal *** was especially the case for binding to the opportunistic pathogenic species Serratia marcescens;the nematodes’bacteria binding ability at this critical stage when it infiltrates Ma for dispersal was much weaker compared with Caenorhabditis elegans,Diplogasteroides asiaticus,and propagative-stage *** associated bacterium ***,which was isolated from the beetles’pupal chambers,was unfavorable to Ma,because it caused a higher mortality rate upon injection into *** addition,*** in the tracheae caused more immune effector disorders compared with PWN ***_Galectin2(MaGal2),a pattern-recognition receptor,was up-regulated following PWN *** MaGal2 protein formed aggregates with five dominant associated bacteria in ***,MaGal2 knockdown beetles had up-regulated prophenoloxidase gene expression,increased phenoloxidase activity,and decreased PWN *** study revealed a previously unknown strategy for immune evasion of this plant pathogen inside its vector,and provides novel insights into the role of bacteria in parasite–host interactions.
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