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Measuring the number concentration of arbitrarily-shaped gold nanoparticles with surface plasmon resonance microscopy

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Science China Chemistry 2016年第7期59卷 843-847页

作者： Xiang Wo Yashuang Luo Nongjian Tao Wei Wang Hong-Yuan Chen State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical Engineering Nanjing University Nanjing 210023 China

Molar concentration of gold nanoparticles is one of the most critical parameters of gold colloids in order to develop their applications in sensing, diagnostics and nanomedicine. Previous methods often stand just for gold nanoparticles with regular shape and narrow size distribution. In the present work, we proposed an absolute quantification method that determined the molar concentration of gold nanoparticles with arbitrary shapes and polydisperse sizes. This approach involved the real time monitoring and counting of individual nanoparticles collision events, from which the quantification of molar concentration was achieved using a theoretical model consisting of Fiek＇s laws of diffusion and Stokes-Einstein equation. The determination of spherical gold nanoparticles concentration resulted in excellent agreement with traditional spectrometry method. It was further demonstrated that the present approach can be expanded to determine the molar concentration of gold nanoparticles with arbitrary shapes and poly-diversed distributions.

关键词： number concentration gold nanoparticles surface plasmon resonance microscopy

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A novel detection of single-stranded DNA binding protein based on ss-DNA modified chip using surface plasmon resonance microscopy

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Chinese Chemical Letters 2007年第4期18卷 441-444页

作者： Jian Quan Lu Ming Bo Xu Xing Wang Zhou Jin Guang Xu Qing Tao Hubei Key Laboratory of Bioanalytical Technique Hubei Normal University Huangshi 435002 China Department of Chemistry Central China Normal University Wuhan 430079 China

An ss-DNA gold chip was prepared based on self-assembly of the thiol-derivatized oligonucleotide, and used for the determination of single-stranded binding protein （SSB） by surface plasmon resonance microscopy （SPR）. The experiment results showed that SSB binds ss-DNA with high specificity, and relative signal of SPR response is proportional to the concentration of SSB in the range of 0.1-100 ng/mL with a detection limit （S/N = 3） of 0.07 ng/mL.

关键词： Detection Single-stranded DNA binding protein ss-DNA surface plasmon resonance microscopy

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Tracking the Rotational Dynamics of Single Nanorods with surface plasmon resonance microscopy

Tracking the Rotational Dynamics of Single Nanorods with Sur...

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第二十届全国光散射学术会议（CNCLS20）

作者： Yingyan Jiang Wei Wang School of Chemistry and Chemical Engineering Nanjing University

Objective-based surface plasmon resonance microscopy（SPRM） is a wide-field imaging technique that measures the dielectric constant of single nanoparticles based on its interaction with surface plasmon polaritons（SPPs） [1].Because the image contrast of single nanoparticle is dependent on its dielectric constant,a physical property that applies to any material,SPRM is thus suitable for studying all kinds of *** shows a wave-like point spread function（PSF） containing parabolic tails [2].The PSF is determined by the metal film,wavelength and angle of incident light,refractive index of surrounding *** complexity of the wave-like pattern hampered the quantitative interpretation on the PSF of *** applying a 2-dimensional Fourier transform to such patterns,two adjacent rings were observed in the frequency domain.A ring fitting method was then developed to extract geometrical features and to connect these features with several experimental *** addition,when a nanorod takes a non-perpendicular orientation to the SPPs,the wave-like pattern becomes *** dependence of the morphology and orientation of a nano-object on its SPRM image offers a novel technique for studying the rotational dynamics of anisotropic nanomaterials with arbitrary chemical *** proposed an angular spectrum approach to extract the orientation of single CdS nanorods from its SPRM *** was found that the asymmetrical wave-like SPRM image resulted in a peak in its angular spectrum in the frequency *** between the peak angle and the geometrical orientation of nanorod was validated by both in situ scanning electron microscope characterizations and theoretical *** results of real-time monitoring uncovered an accelerated rotation of single CdS nanorods during photocatalysis,which was strong enough to trigger the rotation of a previously-fixed nanocatalyst [3].

关键词： SPPs PSF surface plasmon resonance microscopy nanomotor

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Enrichment of surface charge contributes to the stability of surface nanobubbles

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Particuology 2023年第10期81卷 128-134页

作者： Aosheng Chang Ben Niu Jia Liu Haoran Han Zhibing Zhang Wei Wang School of Chemistry and Chemical Engineering Nanjing UniversityNanjing210023China

surface nanobubbles are spontaneously formed at the interface between hydrophobic surfaces and aqueous solutions,which show extraordinarily longer lifetime than that was predicted by the classical thermodynamics *** the present work,by using a surface plasmon resonance microscopy(SPRM)to quantitatively measure the dissolution kinetics of individual surface nanobubbles in real time,we explored the effects of ionic strength and pH value on the dissolution rates(lifetime)of *** results revealed that nanobubbles could exist stably for a long time in low-concentration electrolyte solutions or high-concentration non-electrolyte solutions,while they dissolved quickly in highconcentration electrolyte *** the increase of ionic strength,the dissolution rates were accelerated by 2-3 orders of magnitude,and thus the lifespan of these surface nanobubbles was significantly *** addition to ionic strength,it was further found that,with the increase of acidity or alkalinity of the solution,the dissolution rates of the surface nanobubbles were faster than that in neutral *** results demonstrated that the interfacial charge enrichment significantly contributed to the extraordinary stability of the surface nanobubbles.

关键词： surface nanobubbles surface plasmon resonance microscopy Electrostatic interaction Dissolution kinetics

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Polystyrene Based SPR Biosensor Chip for Use in Immunoassay

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Tsinghua Science and Technology 1999年第3期4卷 1495-1498页

作者：杨军隋森芳 Department of Biological Sciences and Biotecbnology Tsinghua University Beijing 100084

Biosensors are widely used in immunoassay. The biosensor chip carries a receptor which is used in immunoassay and the chip properties have an important influence on the detecting sensitivity of the biosensor. This paper describes a polystyrene\|based biosensor chip developed and used as part of a surface plasmon resonance (SPR) biosensor. The SPR biosensor has a much higher detecting sensitivity than enzyme\|linked immunoserbent assay (ELISA).

关键词： surface plasmon resonance biosensor polystyrene surface plasmon resonance microscopy enzyme\|linked immunoserbent assay

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