A novel detection of single-stranded DNA binding protein based on ss-DNA modified chip using surface plasmon resonance microscopy

作     者：Jian Quan Lu Ming Bo Xu Xing Wang Zhou Jin Guang Xu Qing Tao 

作者机构：Hubei Key Laboratory of Bioanalytical Technique Hubei Normal University Huangshi 435002 China Department of Chemistry Central China Normal University Wuhan 430079 China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2007年第18卷第4期

页      面：441-444页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 0703[理学-化学] 

基　　金：the Science Foundation of the National Education Ministry (No, 206096) the Education Department of Hubei Province (No. Z200522002) 

主　　题：Detection Single-stranded DNA binding protein ss-DNA Surface plasmon resonance microscopy 

摘      要：An ss-DNA gold chip was prepared based on self-assembly of the thiol-derivatized oligonucleotide, and used for the determination of single-stranded binding protein （SSB） by surface plasmon resonance microscopy （SPR）. The experiment results showed that SSB binds ss-DNA with high specificity, and relative signal of SPR response is proportional to the concentration of SSB in the range of 0.1-100 ng/mL with a detection limit （S/N = 3） of 0.07 ng/mL.