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MRI tracking of human Wharton’s jelly stem cells seeded onto acellular dermal matrix labeled with superparamagnetic iron oxide nanoparticles in burn wounds

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Burns & Trauma 2022年第1期10卷 129-141页

作者： Davood Mehrabani Mehra Nazempour Rouhollah Mehdinavaz-Aghdam Seyedeh-Sara Hashemi Reza Jalli Mahdi Saeedi Moghadam Shahrokh Zare Iman Jamhiri Javad Moayedi Feridoun Karimi-Busheri Stem Cell Technology Research Center Shiraz University of Medical SciencesShirazFars T6G 2E171348-14336Iran Burn and Wound Healing Research Center Shiraz University of Medical SciencesShirazFars 71987-74731Iran Comparative and Experimental Medicine Center Shiraz University of Medical SciencesShirazFars 71348-14336Iran Li Ka Shing Center for Health Research and Innovation University of AlbertaEdmontonAlbertaCanada Department of Biomedical and Tissue Engineering Science and Research BranchIslamic Azad UniversityTehranIran School of Metallurgy and Materials Engineering College of EngineeringUniversity of TehranTehran ***Iran Medical Imaging Research Center Department of RadiologyShiraz University of Medical SciencesShirazFars 71348-14336Iran Department of Biochemistry School of Biotechnology and AgricultureShiraz BranchIslamic Azad UniversityShirazFars 71987-74731Iran Department of Oncology Faculty of MedicineUniversity of AlbertaEdmontonAlbertaT6G 1Z2Canada

Background:In vivo cell tracking after transplantation in regenerative medicine remains an unmet challenge and limits current understanding of the wound healing mechanism through cell-based *** study investigated tracking of human Wharton’s jelly stem cells(hWJSCs)seeded onto an acellular dermal matrix(ADM)and labeled with superparamagnetic iron oxide nanoparti-cles(SPIONs)by magnetic resonance imaging(MRI)in burn ***:The hWJSCs were characterized and assessed for growth kinetics.A total of 30 rats were enrolled in three equal *** 1 underwent scald burn injury left without treatment,the group 2 was treated by an ADM that was prepared from cosmetic surgery skin samples and the group 3 received hWJSCs labeled with SPIONs seeded onto an *** strength was evaluated before and after interventions,real time PCR assessed apoptosis,and Prussian blue staining,scanning electron microscopy(SEM)and MRI were used for the tracking of labeled ***:The hWJSCs exhibited mesenchymal stem cell *** doubling time was 40.1 *** did not show any toxic *** hWJSCs seeded onto an ADM decreased Bax and increased Bcl-2 gene *** of SPIONs within hWJSCs was confirmed by Prussian blue staining,SEM and MRI until day *** was a significant difference between the Young’s moduli of normal skin and the group receiving hWJSCs seeded onto an *** observations and SEM imaging confirmed that MRI is an accurate method to track SPION-labeled hWJSCs in ***:This study showed that SPION labeling coupled with MRI can be used to further understand the fate of stem cells after transplantation in a burn model.

关键词： Wharton’s jelly stem cells superparamagnetic iron oxide nanoparticles Acellular dermal matrix Magnetic resonance imaging Healing Burn

Recent Progress in superparamagnetic iron oxide nanoparticles

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农业生物技术:英文版 2023年第2期12卷 121-126,137页

作者： Qi NIE Jian WEN College of Pharmacy Guilin Medical UniversityGuilin 541004China Affiliated Hospital of Guilin Medical University Guilin 541004China

superparamagnetic iron oxide nanoparticles(SPIONs)have immeasurable potentials in many fields such as nanobiotechnology and biomedical engineering because of their superparamagnetic properties and small particle *** review introduces the methods for SPIONs synthesis,including co-precipitation,thermal decomposition,microemulsion and hydrothermal reaction,and surface modification of SPIONs with organometallic and inorganic metals,surface modification for targeted drug delivery,and the use of SPIONs as a contrast *** addition,this article also provides an overview of recent progress in SPIONs for the treatment of glioma,lung cancer and breast cancer.

关键词： superparamagnetic iron oxide nanoparticles Tumor therapy Synthesis Surface modification Contrast agent

Dextran coated superparamagnetic iron oxide nanoparticles activate human monocyte cells via MAPK signaling pathway

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Dextran coated superparamagnetic iron oxide nanoparticles ac...

Immunoreactivity Comparison of Different superparamagnetic iron oxide nanoparticles According to Their Ability to Activate the Human Complement System

第十一届全国免疫学学术大会

作者： Wu QiHong Feng Ting Miao TianYu Guo YingKun Li Hong Key Laboratory of Obstetrics&Gynecology and Pediatric Disease and Birth Defects of Ministry of Education West China Second University HospitalSichuan University West China University Hospital Sichuan University

superparamagnetic iron oxide nanoparticles(SPION) have great biocompatibility, high magnetic resonance, and low toxicity, resulting in the rapid advance of SPION. With the increase of SPION applications in biomedicine, it is very necessary to assess the biological toxicity of SPION, especially with human innate immune system. In this study, we investi-gated the effects of dextran coated-SPION(Dex-SPION) on human monocyte cells by transmission electron microscopy, flow cytometroy, enzyme-linked immuno sorbent assay and western blot. Our results showed that the Dex-SPION localised in phagosome or free in the cytoplasm of human monocyte cells, and which did not affect the viability. However, the phenotypes(CCR2, HLA-DR, CD163 and CD206) tested of monocytes were changed and proinflammatory cytokines(IL-1β and TNF-α) levels were increased, suggesting the activation of human monocyte cells. Additionally, phosphorylation of mitogen-activated protein kinase(MAPK), p38, JNK, ERK were evaluated after Dex-SPION treatment and MAPK inhibitors obviously inhibit the production of proinflammatory cytokines. In summary, the obtained results suggested that Dex-SPION could activate human monocyte cells and that activation of MAPK pathway were possibly involved in these effects. Our data demonstrated that there exist a interaction between human monocyte cells and DexSPION, which should be thoughtful when deciding their various applications in biomedicine.

关键词： superparamagnetic iron oxide nanoparticles Human monocyte cells Inflammation Mitogen-activated pr

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Immunoreactivity Comparison of Different Superparamagnetic I...

superparamagnetic iron oxide nanoparticle targeting of adipose tissue-derived stem cells in diabetes-associated erectile dysfunction

第十二届全国免疫学学术大会

作者： Li Jincheng Wang Zhaoyi Chen Fangfang Fang Xuedong China-Japan Union Hospital Jilin University Department of Chemistry Jilin University

The complement proteins deposit on the surface of nanoparticles prime the particle for removal by immune cells and may contribute toward infusion-related adverse effects such as allergic *** of the core issues of nanotechnology involves masking the foreignness of nanomaterials to enable in vivo longevity and long-term immune ***,we synthesized three different types of nanoparticles,which are 20 kDa dextran-coated superparamagnetic iron oxide core-shell nanoparticles(SPIO),cross-linked iron oxide nanoparticles(CLIO),and amino-modined iron oxide nanoparticles(A-CLIO),and compared their *** alternative pathway of the complement system is initiated through spontaneous auto-activation of soluble C3,or when the internal thioester in the a’ chain of nascent C3 b reacts with hydroxyl or amino groups on a foreign ***,we incubated SPIO,CLIO,and A-CLIO in human serum or lepirudin-plasma resulted in a potent opsonization with C3 *** EDTA and anti-properdin antibody added serum was used as control experimental *** show that complement opsonization was completely blocked with EDTA and partially blocked with anti-properdin *** investigate the immunoreactivity,the nanoparticles were incubated with serum or plasma and then added to human *** efficiency of uptake was determined as the number of magnetically labelled leucocytes isolated over the magnetic *** turned out that A-CLIO demonstrated the highest immunoreactivity,while SPIO and CLIO behave similar in the activation process.

关键词： superparamagnetic iron oxide nanoparticles immunoreactivity complement activation

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Asian Journal of Andrology 2017年第4期19卷 425-432页

作者： Lei-Lei Zhu Zheng Zhang He-Song Jiang Hai Chen Yun Chen Yu-Tian Dai Department of Andrology Drum Tower Hospital School of Medicine Nanjing University Nanjing 210008 China

Erectile dysfunction （ED） is a major complication of diabetes, and many diabetic men with ED are refractory to common ED therapies. Adipose tissue-derived stem cells （ADSCs） have been shown to improve erectile function in diabetic animal models. However, inadequate cell homing to damaged sites has limited their efficacy. Therefore, we explored the effect of ADSCs labeled with superparamagnetic iron oxide nanoparticles （SPIONs） on improving the erectile function of streptozotocin-induced diabetic rats with an external magnetic field. We found that SPIONs effectively incorporated into ADSCs and did not exert any negative effects on stem cell properties. Magnetic targeting of ADSCs contributed to long-term cell retention in the corpus cavernosum and improved the erectile function of diabetic rats compared with ADSC injection alone. In addition, the paracrine effect of ADSCs appeared to play the major role in functional and structural recovery. Accordingly, magnetic field-guided ADSC therapy is an effective approach for diabetes-associated ED therapy.

关键词： erectile dysfunction magnetic targeting stem cells superparamagnetic iron oxide nanoparticles

Extracellular magnetic labeling of biomimetic hydrogel-induced human mesenchymal stem cell spheroids with ferumoxytol for MRI tracking

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Bioactive Materials 2023年第1期19卷 418-428页

作者： Sen Yan Ke Hu Miao Zhang Jingyi Sheng Xueqin Xu Shijia Tang Yan Li Sheng Yang Guangxiang Si Yu Mao Yi Zhang Feimin Zhang Ning Gu State Key Laboratory of Bioelectronics Jiangsu Key Laboratory for Biomaterials and DevicesSchool of Biological Science and Medical EngineeringSoutheast UniversityNanjing210009JiangsuChina Laboratory of Oral Regenerative Medicine Technology School of Biomedical Engineering and InformaticsDepartment of Biomedical EngineeringNanjing Medical UniversityNanjing211166JiangsuChina Jiangsu Key Laboratory of Oral Diseases Affiliated Hospital of StomatologyNanjing Medical UniversityNanjing210029JiangsuChina Department of Biomedical Engineering School of MedicineTsinghua UniversityBeijing100084China Department of Colorectal Surgery The First Affiliated Hospital of Nanjing Medical UniversityNanjing210000JiangsuChina

Labeling of mesenchymal stem cells(MSCs)with superparamagnetic iron oxide nanoparticles(SPIONs)has emerged as a potential method for magnetic resonance imaging(MRI)tracking of transplanted cells in tissue repair studies and clinical *** of MSCs using clinically approved SPIONs(ferumoxytol)requires the use of transfection reagents or magnetic field,which largely limits their clinical *** overcome this obstacle,we established a novel and highly effective method for magnetic labeling of MSC spheroids using *** conventional methods,ferumoxytol labeling was done in the formation of a mechanically tunable biomimetic hydrogel-induced MSC ***,the labeled MSC spheroids exhibited strong MRI T2 signals and good ***,the encapsulated ferumoxytol was localized in the extracellular matrix(ECM)of the spheroids instead of the cytoplasm,minimizing the cytotoxicity of ferumoxytol and maintaining the viability and stemness properties of biomimetic hydrogel-induced MSC *** demonstrates the potential of this method for post-transplantation MRI tracking in the clinic.

关键词： superparamagnetic iron oxide nanoparticles MSC labeling Mechanically tunable biomimetic hydrogel Spheroids MRI tracking

Magnetic nanoparticles conjugated with “RPE cell-MCP-1 antibody-VEGF antibody” compounds for the targeted therapy of age-related macular degeneration: a hypothesis

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International Journal of Ophthalmology(English edition) 2017年第5期10卷 812-814页

作者： Zhao-Jiang Du Peng Li Li Wang Department of Ophthalmology Tangdu Hospital the Fourth Military Medical University Department of Ophthalmology No.451 Hospital of PLA Department of Optometry Xi'an Medical University

Age-related macular degeneration（AMD） is the leading cause of vision loss in the elderly throughout the world. Treatment of AMD utilizing retinal pigment epithelium（RPE） transplantation represents a promising therapy. However, simplex RPE transplantation can only replace the diseased RPE cells, but has no abilities to stop the development of AMD. It has been indicated that oxidization triggers the development of AMD by inducing the dysfunction and degeneration of RPE cells, which results in the upregulation of local monocyte chemotactic protein-1（MCP-1） expression. MCP-1 induces macrophage recruiment which triggers local inflammation. As a result, the expression of vascular endothelial growth factor（VEGF） is upregulated by MCP-1mediated inflammation and results in the formation of choroidal neovascularization（CNV）. We accordingly propose a targeted therapy of AMD by subretinal transplanting the compound of RPE cell, MCP-1 antibody, and VEGF antibody and using a magnetic system to guide RPE cell compounds conjugated with superparamagnetic iron oxide nanoparticles（SPIONs）. Furthermore, SPION-labelled RPE cells can be tracked and detected in vivo by non-invasive magnetic resonance imaging（MRI）. This novel RPE cell transplantation methodology seems very promising to provide a new therapeutic approach for the treatment of AMD.

关键词： age-related macular degeneration retinal pigment epithelium superparamagnetic iron oxide nanoparticles RPE cell transplantation targeted therapy