MRI tracking of human Wharton’s jelly stem cells seeded onto acellular dermal matrix labeled with superparamagnetic iron oxide nanoparticles in burn wounds

作     者：Davood Mehrabani Mehra Nazempour Rouhollah Mehdinavaz-Aghdam Seyedeh-Sara Hashemi Reza Jalli Mahdi Saeedi Moghadam Shahrokh Zare Iman Jamhiri Javad Moayedi Feridoun Karimi-Busheri 

作者机构：Stem Cell Technology Research CenterShiraz University of Medical SciencesShirazFars T6G 2E171348-14336Iran Burn and Wound Healing Research CenterShiraz University of Medical SciencesShirazFars 71987-74731Iran Comparative and Experimental Medicine CenterShiraz University of Medical SciencesShirazFars 71348-14336Iran Li Ka Shing Center for Health Research and InnovationUniversity of AlbertaEdmontonAlbertaCanada Department of Biomedical and Tissue EngineeringScience and Research BranchIslamic Azad UniversityTehranIran School of Metallurgy and Materials EngineeringCollege of EngineeringUniversity of TehranTehran ***Iran Medical Imaging Research CenterDepartment of RadiologyShiraz University of Medical SciencesShirazFars 71348-14336Iran Department of BiochemistrySchool of Biotechnology and AgricultureShiraz BranchIslamic Azad UniversityShirazFars 71987-74731Iran Department of OncologyFaculty of MedicineUniversity of AlbertaEdmontonAlbertaT6G 1Z2Canada 

出 版 物：《Burns & Trauma》 (烧伤与创伤（英文）)

年 卷 期：2022年第10卷第1期

页      面：129-141页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Burn and Wound Healing Research Center Comparative and Experimental Center Iran Ministry of Health, Treatment and Education National Institute for Medical Research Development of Iran Ministry of Health, Treatment and Medical Education, (963474) Stem Cell Technology Research Center 

主　　题：Wharton’s jelly stem cells Superparamagnetic iron oxide nanoparticles Acellular dermal matrix Magnetic resonance imaging Healing Burn 

摘      要：Background:In vivo cell tracking after transplantation in regenerative medicine remains an unmet challenge and limits current understanding of the wound healing mechanism through cell-based *** study investigated tracking of human Wharton’s jelly stem cells(hWJSCs)seeded onto an acellular dermal matrix(ADM)and labeled with superparamagnetic iron oxide nanoparti-cles(SPIONs)by magnetic resonance imaging(MRI)in burn ***:The hWJSCs were characterized and assessed for growth kinetics.A total of 30 rats were enrolled in three equal *** 1 underwent scald burn injury left without treatment,the group 2 was treated by an ADM that was prepared from cosmetic surgery skin samples and the group 3 received hWJSCs labeled with SPIONs seeded onto an *** strength was evaluated before and after interventions,real time PCR assessed apoptosis,and Prussian blue staining,scanning electron microscopy(SEM)and MRI were used for the tracking of labeled ***:The hWJSCs exhibited mesenchymal stem cell *** doubling time was 40.1 *** did not show any toxic *** hWJSCs seeded onto an ADM decreased Bax and increased Bcl-2 gene *** of SPIONs within hWJSCs was confirmed by Prussian blue staining,SEM and MRI until day *** was a significant difference between the Young’s moduli of normal skin and the group receiving hWJSCs seeded onto an *** observations and SEM imaging confirmed that MRI is an accurate method to track SPION-labeled hWJSCs in ***:This study showed that SPION labeling coupled with MRI can be used to further understand the fate of stem cells after transplantation in a burn model.