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Method of lateral image reconstruction in structured illumination microscopy with super resolution

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Journal of Innovative Optical Health Sciences 2016年第3期9卷 4-18页

作者： Qiang Yang Liangcai Cao Hua Zhang Hao Zhang Guofan Jin State Key Laboratory of Precision Measurement Technology and Instruments Tsinghua University Beijing 100084P.R.China

The image reconstruction process in super-resolution structured illumination microscopy(SIM)is *** structured pattern is generated by the interference of two Gaussian beams to encode undetectable spectra into detectable region of *** parameters estimation of the structured pattern,the encoded spectra are computationally decoded and recombined in Fourier domain to equivalently increase the cut-off frequency of microscope,resulting in the extension of detectable spectra and a reconstructed image with about two-fold enhanced *** di®erent methods to estimate the initial phase of structured pattern are compared,verifying the auto-correlation algorithm a®ords the fast,most precise and robust *** artifacts sources and detailed reconstruction°owchart for both linear and nonlinear SIM are also presented.

关键词： Microscopy structured illumination super-resolution image reconstruction

A lateral super-resolution imaging method using structured illumination without phase shift

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Nanotechnology and Precision Engineering 2019年第3期2卷 130-137页

作者： Yuan Jia Junsheng Lu Xinyu Chang Xiaodong Hu School of Precision Instruments&Opto-Electronics Engineering Tianjin UniversityTianjin 300072China State Key Laboratory of Precision Measuring Technology and Instruments Tianjin UniversityTianjin 300072China

structured illumination microscopy has been a useful method for achieving lateral super-resolution,but it typically requires at least three precise phase shifts per *** this paper,we propose a super-resolution method that utilizes structured illumination without phase *** reconstruction process requires only a conventionally illuminated image and an image with structured *** method achieves the same effect as the traditional phase shift method,and more than doubles the resolution by synthesizing a few reconstructions at different illumination *** verify the resolution improvement process using a combination of theoretical derivations and diagrams,and demonstrate its effectiveness with numerical simulations.

关键词： Super-resolution structured illumination Reconstruction Non phase shift

Fringe optimization for structured illumination super-resolution microscope with digital micromirror device

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Journal of Innovative Optical Health Sciences 2019年第3期12卷 78-86页

作者： Xibin Yang Qian Zhu Zhenglong Sun Gang Wen Xin Jin Linbo Wang Jialin Liu Daxi Xiong Hui Li Jiangsu Key Lab of Medical Optics Suzhou Institute of Biomedical Engineering and Technology Chinese Academy of Sciences No.88 Keling Street Suzhou 215163P.R.China University of Chinese Academy of Sciences Beijing 100049 P.R.China

structured illumination microscopy(SIM)is a promising super-resolution technique for imaging subcellular structures and dynamics due to its compatibility with most commonly usedffuorescent labeling *** illumination can be obtained by either laser interference or projection of fringe ***,we proposed a fringe projector composed of a compact multiwavelength LEDs module and a digital micromirror device(DMD)which can be directly attached to most commercial invertedffuorescent microscopes and update it into a SIM *** effects of the period and duty cycle of fringe patterns on the modulation depth of the structured lightfield were *** the optimized fringe pattern,1:6×resolution improvement could be obtained with high-end oil *** imaging and dynamics of subcellular organelles in live cells were also *** method provides a low-cost solution for SIM setup to expand its wide range of applications to most research labs in thefield of life science and medicine.

关键词： structured illumination super-resolution digital micromirror device fringe pattern modulation depth

Miniaturization of a coherent monocular structured illumination system for future combination with digital holography

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Light(Advanced Manufacturing) 2022年第3期3卷 160-167页

作者： Andreas Walter Stark Eugene Wong Holger Babovsky Christian Franke Richard Kowarschik Institute of Applied Optics and Biophysics Friedrich Schiller University Jena07743 JenaGermany University Hospital 07743 JenaGermany

Holographic and 3D-measurement processes are an often-used tool in industry,medicine,and scientific *** small deviations of objects can be visualized by holographic means with high accuracy,optical systems with active structured illumination are a reliable source of absolute 3D-information in these *** combination of digital holography with structured illumination allows to simultaneously measure deformations and absolute 3D coordinates but also requires coherent light and has already been demonstrated in principle with a stereo camera ***-camera systems are limited to certain setup sizes given by the volume and distance of the *** the system to a one-camera(monocular)setup reduces space and acquisition *** using a multi-aperture illumination source an extremely high projection rate could be realized and reduced to a monocular approach with a novel voxel-calibration technique,while the projection system itself still requires a large amount of *** this paper we present a miniaturized,monocular 3D-measurement system that works with repeatable,coherent speckles,generated by a fiber-coupled laser whose light was distributed by a fiber-switch to a diffuser plate connected with a measurement-head,also including a *** addressing different fibers through the switch,varying but repeatable patterns are *** size of the device(diameter<3 cm)is now mainly limited by the volume of the camera.A first 3D-reconstruction of an object and an outlook for a combination of this system with digital holography is given,allowing absolute 3D-coordinates and relative deviations of object points to be measured simultaneously.

关键词： 3D-measurement structured illumination Speckle projection Monocular 3D-measurement Combination 3D-measurment and holography

Confocal rescan structured illumination microscopy for real-time deep tissue imaging with superresolution

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Advanced Photonics Nexus 2023年第1期2卷 80-88页

作者： Shuhao Shen E Du Miao Zhang Yuting Wen Kai Long Anqi Qiu Nanguang Chen National University of Singapore Department of Biomedical EngineeringSingapore Xidian University Guangzhou Institute of TechnologyGuangzhouChina Shenzhen Institute of Information Technology School of MicroelectronicsShenzhenChina National University of Singapore(Suzhou)Research Institute SuzhouChina

structured illumination microscopy(SIM)is an established optical superresolution imaging ***,conventional SIM based on wide-field image acquisition is generally limited to visualizing thin cellular *** propose combining one-dimensional image rescan and structured illumination in the orthogonal direction to achieve superresolution without the need to rotate the illumination *** image acquisition speed is consequently improved threefold,which is also beneficial for minimizing photobleaching and *** sectioning in thick biological tissue is enhanced by including a confocal slit in the system to significantly suppress the out-of-focus background and the associated *** all the technical improvements,our method captures threedimensional superresolved image stacks of neuronal structures in mouse brain tissue samples for a depth range of more than 200μm.

关键词： superresolution deep tissue imaging structured illumination microscopy image rescan