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Optimization of Fermentation Process for Hyaluronic Acid Production by streptococcus zooepidemicus

Agricultural Biotechnology 2015年第5期4卷 56-60页

作者： Jing YUAN Zhiping ZHAO Baoqu WANG Lingli WU Li ZHANG School of Chemistry and Pharmaceutical Engineering Sichuan University of Science & Engineering Institute of Pharmaceutical Engineering Technology and Application Sichuan University of Science & Engineering

Hyaluronic acid （HA） is a high molecular weight glycosaminoglycan consisting of alternating D-glucuronic acid and N-acetylglueasamine and plays ex- tremely important roles in many biological processes. In this study, we optimized fermentation process for the production of HA by streptococcus zooepidemicus ATCC35246, including fermentation broth composition and various fermentation parameters. The experimental results showed that the optimal fermentation broth composition was： glucose 45 g/L, yeast extract 10 g/L, tryptone 12 g/L, KH2PO4 2 g/L, K2HPO4 . 3H20 2 g/L, MgSO4 · 7H2O 2 g/L, and （NH4 ）2SO4 0.4 g/L. The optimal parameters involved in fermentation was： liquid volume 20%, pH 6. 0, rotation speed 180 r/min, fermentation temperature 35 ℃, fermentation duration 18 h, CTAB concentration 25 mg/L. Under the optimized conditions, the yield of HA was 0. 305 g/L, which was dramatically improved by 43.87% compared to that of 0. 212 g/L before optimization.

关键词： streptococcus zooepidemicus Hyaluronic acid Fermentation process Optimization

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Purification and Structure Identification of Hyaluronic Acid

引用

Chinese Chemical Letters 2004年第7期15卷 811-812页

作者： ShuiShengHONG JiaCHEN JianGuoZHANG YanChunTAO LanYingLIU CollegeofLifeScience JilinUniversityChangchun130023 StateKeyLaboratoryofElectroanalyticalChemistry ChineseAcademyofSciencesChangchunInstituteofAppliedChemistryChangchun130022 KeyLaboratoryofSupermolecularStructureandSpectroscopyofEducationalMinistry JilinUniversityChangchun130023

Polysaccharide produced by mutated strain of streptococcus zooepidemicus was purified by the procedures including Savage method, quaternary ammonium compound precipitation, DEAE-cellulose(DE52) chromatography and Sephadex G-75 gel filtration. The structure of the purified polysaccharide has been characterized by means of chemical composition analysis,13C NMR spectrum, infrared spectrum and circular dichroism (CD). All the results showed that the purified polysaccharide was hyaluronic acid (HA). The single helix conformation of the purified HA was determined by Congo red experiment. The molecular weight of the HA was about 1.16×106D, which was measured by viscosity method.

关键词： streptococcus zooepidemicus hyaluronic acid structure identification.

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