Optimization of Fermentation Process for Hyaluronic Acid Production by Streptococcus zooepidemicus

作     者：Jing YUAN Zhiping ZHAO Baoqu WANG Lingli WU Li ZHANG 

作者机构：School of Chemistry and Pharmaceutical EngineeringSichuan University of Science & Engineering Institute of Pharmaceutical Engineering Technology and ApplicationSichuan University of Science & Engineering 

出 版 物：《Agricultural Biotechnology》 (农业生物技术（英文版）)

年 卷 期：2015年第4卷第5期

页      面：56-60页

学科分类：081703[工学-生物化工] 08[工学] 0817[工学-化学工程与技术] 0836[工学-生物工程] 082203[工学-发酵工程] 0822[工学-轻工技术与工程] 

基　　金：Supported by Scientific Research Fund of Sichuan University of Science&Engineering(2011RC12,2014KY02) Scientific Research Foundation of the Education Department of Sichuan Province(15ZA0222) Research Project of Liquor Making Biological Technology and Application of Key Laboratory of Sichuan Province(NJ2013-06) Sichuan Provincial Undergraduate Training Programs for Innovation and Entrepreneurship(201410622021) 

主　　题：Streptococcus zooepidemicus Hyaluronic acid Fermentation process Optimization 

摘      要：Hyaluronic acid （HA） is a high molecular weight glycosaminoglycan consisting of alternating D-glucuronic acid and N-acetylglueasamine and plays ex- tremely important roles in many biological processes. In this study, we optimized fermentation process for the production of HA by Streptococcus zooepidemicus ATCC35246, including fermentation broth composition and various fermentation parameters. The experimental results showed that the optimal fermentation broth composition was： glucose 45 g/L, yeast extract 10 g/L, tryptone 12 g/L, KH2PO4 2 g/L, K2HPO4 . 3H20 2 g/L, MgSO4 · 7H2O 2 g/L, and （NH4 ）2SO4 0.4 g/L. The optimal parameters involved in fermentation was： liquid volume 20%, pH 6. 0, rotation speed 180 r/min, fermentation temperature 35 ℃, fermentation duration 18 h, CTAB concentration 25 mg/L. Under the optimized conditions, the yield of HA was 0. 305 g/L, which was dramatically improved by 43.87% compared to that of 0. 212 g/L before optimization.