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streptococcus agalactiae:Identification methods,antimicrobial susceptibility, and resistance genes in pregnant women

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World Journal of Clinical Cases 2020年第18期8卷 3988-3998页

作者： Fabrícia Almeida Fernandes Santana Tais Viana Ledo de Oliveira Marcelo Barreto de Souza Filho Lucas Santana Coelho da Silva Breno Bittencourt de Brito Fabrício Freire de Melo Cláudio Lima Souza Lucas Miranda Marques Márcio Vasconcelos Oliveira Instituto Multidisciplinar em Saúde Universidade Federal da BahiaVitória da Conquista 45029-094BahiaBrazil Laboratório Oliveira Ltda LABOVitória da Conquista 45020740BahiaBrazil Campus Soane Nazaréde Andrade Universidade Estadual de Santa CruzIlhéus 45662900BahiaBrazil

BACKGROUND Group B streptococcus(GBS)is a normal component of the gastrointestinal and genital microbiota in humans and can lead to important infections in *** To compare GBS isolation and identification methods as well as to assess the antibiotic susceptibility and to identify resistance genes in GBS strains from pregnant women attended in healthcare services from the city of Vitória da Conquista,in Bahia State,*** From January 2017 to February 2018,vaginorectal swabs were obtained from 186 participants and the samples were seeded onto chromogenic agar for GBS before and after inoculation in selective *** identification using 3 CAMP and latex tests was performed in samples with GBS-suggestive ***,disk diffusion antibiograms were performed in GBS-positive samples,and the detection of the resistance genes ermB,ermTR,mefA,and linB in the clindamycin and/or erythromycin-resistant samples was carried *** Thirty-two samples(17.2%)were *** culture in chromogenic agar after sample incubation in selective broth was the most sensitive method(96.9%)for GBS *** isolates were susceptible to penicillin,ampicillin,cefotaxime,and *** resistance was observed in 6 samples(18.8%),while 8 samples(25%)were *** erythromycin and/or clindamycin-resistant GBS strains had negative *** strains(25%)presented an M phenotype and 6 isolates(75%)presented a cMLSB *** ermB gene was identified in 4 samples(44.4%),the mefA gene was also found in 4 samples(44.4%),the ermTR gene was identified in 1 isolate(11.1%),and the linB gene was not found in any *** This study evidenced that the screening for SGB can be performed by means of various methods,including chromogenic media,and that the chemoprophylaxis for pregnant women who cannot use penicillin must be susceptibility-guided.

关键词： streptococcus agalactiae Pregnancy Antimicrobial susceptibility

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streptococcus agalactiae Anterior Cervical Space and Superior Mediastinum Abscess in a Liver Transplant Recipient

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Infectious Microbes & Diseases 2019年第2期1卷 75-77页

作者： Derek L.L.Hung Man-Fai Leung Jade L.L.Teng Herman Tse Susanna K.P.Lau Patrick C.Y.Woo Department of Microbiology The University of Hong KongHong KongChina State Key Laboratory of Emerging Infectious Diseases The University of Hong KongHong KongChina Carol Yu Centre for Infection The University of Hong KongHong KongChina Public Health Laboratory Services Branch Centre for Health ProtectionHong Kong Department of HealthThe University of Hong KongHong KongChina Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases The University of Hong KongHong KongChina

Introduction streptococcus agalactiae is most commonly associated with pregnancy-related infections,neonatal sepsis,and infections in patients with immunocompromised states,such as diabetes mellitus and malignancies.1 Although abscesses caused by *** are sometimes encountered in immunocompromised patients,*** abscess in transplant recipients is extremely *** abscesses have never been reported in liver transplant *** this article,we report a case of *** abscess in the anterior cervical space extending to the superior mediastinum in a liver transplant recipient.

关键词： streptococcus agalactiae liver transplant abscess

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The streptococcus agalactiae Ribose Binding Protein B (RbsB) Mediates Quorum Sensing Signal Uptake via Interaction with Autoinducer-2 Signals

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Journal of Ocean University of China 2021年第5期20卷 1285-1295页

作者： FAN Bolin PAN Lixia WANG Zhongliang WANGKAHART Eakapol HUANG Yuchong YANG Dengfeng JIAN Jichang HUANG Yu WANG Bei College of Fishery Guangdong Ocean UniversityGuangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic AnimalKey Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education InstitutesZhanjiang 524088China National Engineering Research Center for Non-Food Biorefinery State Key Laboratory of Non-Food Biomass and Enzyme TechnologyGuangxi Academy of SciencesNanning 536000China Key Laboratory of Experimental Marine Biology Institute of OceanologyChinese Academy of SciencesQingdao 266071China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and Techno-logyQingdao 266237China Research Unit of Excellence for Tropical Fisheries and Technology Division of FisheriesDepartment of Agricultural TechnologyFaculty of TechnologyMahasarakham UniversityKantarawichaiMahasarakhamThailand Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry Guangxi Beibu Gulf Marine Research CenterGuangxi Academy of SciencesNanning 536000China

Understanding aquatic pathogen in sediments or aquacultural water is crucial to protect public health from soilborne and waterborne *** sensing(QS)was increasingly reported in biological wastewater treatment processes because of their inherent roles in biofilm development,bacterial aggregation and so *** widely QS signals was Antoinducer-2(AI-2),primarily involved to allow the possibility of interspecies ***,the cellular components that mediate the response of streptococcus agalactiae to AI-2 have not been fully *** of the complete genome sequence of *** indi-cated that its RbsB protein has similarity to Escherichia coli LsrB and Aggregatibacter actinomycetemcomitans RbsB proteins that bind *** hypothesized that RbsB protein mediates quorum sensing signal uptake via interaction with *** evaluate the regulatory effect of RbsB on QS system,the recombinant plasmid pGEX-6p-1-RbsB was constructed and RbsB protein was purified with *** further elucidate the role of RbsB protein binding to DPD(AI-2 precursor dihydroxypentanedione),the systemati-cally throughput circular dichroism(CD)spectroscopy,isothermal titration calorimetry200(ITC200)and molecular docking methods were *** high expression of soluble RbsB protein with molecular weight of 33 kDa was *** thermodynamics results(ΔH<0,ΔS<0,ΔG<0)with ITC determination indicated that the binding process between DPD and RbsB was exothermic and spontaneous,with hydrogen bonds and van der Waals forces as the main binding ***,DPD can be more easily combined with RbsB in a dose-dependent manner,suggesting that RbsB was changed in the microenvironment of DPD when the DPD concentration was between 0.8-1.0mmolL−1 and reaching the maximum binding *** to molecular docking,3 hydrophobic residues involved in DPD and RbsB protein stable binding were be found,and also hydrogen bonding plays a key role in the formation of th

关键词： streptococcus agalactiae RbsB protein circular dichroism(CD)spectroscopy isothermal titration calorimetry200(ITC200) molecular docking

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In vitro Antibacterial Activity of Palmitoleic Acid Isolated from Filamentous Microalga Tribonema minus Against Fish Pathogen streptococcus agalactiae

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Journal of Ocean University of China 2022年第6期21卷 1615-1621页

作者： WANG Feifei GUO Yuhao CAO Yan ZHANG Chengwu School of Modern Industry for Selenium Science and Engineering Wuhan Polytechnic UniversityWuhan 430023China School of Food Science and Pharmaceutical Engineering Nanjing Normal UniversityNanjing 210023China Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering Ministry of EducationGuangzhou 510632China Department of Ecology and Research Center for Hydrobiology Jinan UniversityGuangzhou 510632China

Filamentous microalgae from genus Tribonema are promising sustainable sources of omega-7 palmitoleic acid,but their ability to accumulate this compound varies among species and depends on the initial nitrogen concentration(INC)*** this study,the palmitoleic acid accumulation capacities of five Tribonema species were examined under three different INCs to select the alga species with the highest *** showed that a high INC was associated with increased palmitoleic acid accumulation but led to decreased biomass concentration in all tested *** particular,*** grown at 18 mmol L^(−1)INC had the highest palmitoleic acid content(20.72%of dry weight)and productivity(90.88 mg L^(−1)d−1).The combination of alkali metal freezing precipitation(AMFP)and urea complexation successfully isolated and enriched palmitoleic acid from *** and obtained a purity of 80.11%and a yield of 7.39 g(100 g)^(−1) of algal *** compound was identified as(9Z)-hexadecenoic acid(C16:1ω-7).Antibacterial activity evaluation for the highly concentrated palmitoleic acid(10 mg mL^(−1))against streptococcus agalactiae revealed the formation of a 12.10 mm-diameter inhibition zone and the minimum inhibitory concentration of 31.25μg mL^(−1),indicating that palmitoleic acid is an effective antibacterial *** study is the first to report that palmitoleic acid derived from *** can antagonize ***,which further broadens the potential application of Tribonema biomass in green aquaculture.

关键词： Tribonema palmitoleic acid enrichment streptococcus agalactiae antibacterial activity

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Trend of Invasive streptococcus agalactiae at Tertiary Care Hospital in Japan

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Journal of Biosciences and Medicines 2016年第3期4卷 15-20页

作者： Masaaki Minami Hideki Nishiyama Shinobu Ikegami Takuya Hattori Makoto Kawashima Michio Ohta Department of Bacteriology Graduate School of Medical Sciences Nagoya City University Nagoya Japan Department of Clinical Laboratory Japanese Red Cross Nagoya Daiichi Hospital Nagoya Japan Department of Clinical Laboratory Japanese Red Cross Nagoya Daini Hospital Nagoya Japan School of Nursing Sugiyama Jyogakuen University Nagoya Japan

streptococcus agalactiae causes various severe infectious diseases such as sepsis, meningitis, and streptococcal toxic shock-like syndrome. Though streptococcus agalactiae infection has been increasing recently, the comprehensive characteristic investigation of invasive streptococcus agalactiae isolated in tertiary care hospitals has not been nearly performed in Japan. In this study, we investigated the clinical characteristics and antimicrobial susceptible patterns of 88 streptococcus agalactiae isolated at two tertiary care hospitals during 2009-2015 in Japan. There was no significant differences between genders in our study. Two-third streptococcus agalactiae were isolated from over age 60. Total mortality rate was 19% and invasive streptococcus agalactiae-associated death cases have occurred every year after 2011. All streptococcus agalactiae were completely susceptible toampicillin. Total non-susceptible rates of erythromycin, minocycline, levofloxacin and trimethoprim-sulfamethoxazole in this study were approximately 30%, 44%, 37%, and 7%, respectively. Our results suggest the need for continuous antimicrobial susceptibility survey of streptococcus agalactiae.

关键词： streptococcus agalactiae Susceptibility Antimicrobial Resistance Epidemiology

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A Pathological Study of GIFT Strain of Nile Tilapia(Oreochromis niloticus)Infected by streptococcus agalactiae

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Agricultural Biotechnology 2018年第5期7卷 136-142页

作者： Wei LUO Xi GAN Jiajie ZHU Qiuwei AO Yun TAN Ming CHEN Yongju LUO Guangxi Academy of Fishery Sciences/Guangxi Key Laboratory for Aquatic Genetic Breeding and Healthy Aquaculture Nanning 530021China

[Objectives] This study aimed to determine the infection pathway and target organs of streptococcus agalactiae in GIFT strain of Nile tilapia, thus providing theoretical basis for the breeding of disease-resistant tilapia and development of S. agalactiae vaccines.[Methods] GIFT strain of Nile tilapia was inoculated by S. agalactiae through intraperitoneal injection, oral gavage and in vitro immersion. The gill, spleen, liver and small intestine tissues of infected tilapia were collected for pathomorphological observation. Immunohistochemical localization was conducted using rabbit anti- S. agalactiae serum to identify the distribution pattern of S. agalactiae in various tissues of tilapia and its target organs via different infection pathways.[Results] GIFT strain of Nile tilapia could be infected by S. agalactiae via three artificial inoculation modes. Specifically, pathological changes occurred at 2 h post-inoculation in intraperitoneal injection and oral gavage groups, whereas tilapia in in vitro immersion group showed pathological changes at 5 h post-inoculation, and the lesion intensity in in vitro immersion group was slighter than that in intraperitoneal injection and oral gavage groups. Immunohistochemical localization indicated that the appearance time of positive signals in intraperitoneal injection group demonstrated an order of spleen→liver and gill→small intestine; positive signals in oral gavage group appeared in the order of small intestine→gill and spleen→liver; the appearance time of positive signals in in vitro immersion group showed an order of gill→spleen→liver and small intestine.[Conclusions] GIFT strain of Nile tilapia could be infected by S. agalactiae via intraperitoneal injection, oral gavage and in vitro immersion. The corresponding positive signals for pathogen infection were preferentially present in the spleen, intestine and gill tissues. Thus, preventing S. agalactiae contamination in aquaculture water and food sources is an effective

关键词： GIFT strain of Nile tilapia streptococcus agalactiae Pathogenic pathway Target organ Immunohistochemistry

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PCR Detection of streptococcus agalactiae in Dairy Cows Suffering from Mastitis

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Agricultural Biotechnology 2013年第5期2卷 22-24页

作者： Yanying ZHANG Guisheng GAO Zhengben LI Guangping GAO Qiumei SHI Xinhua SHAO Yinju LIANG Huiran LIU Key Laboratory of Preventive Veterinary Medicine of Hebei Province College of Animal Science and TechnologyHebei Normal University of Science and Technology Jinfuzhi Dairy Farm of Qian'an City Hebei Xinhua Animal Drug Co. Ltd.

[ Objective ] This study aimed to identify streptococcus agalactiae and lay the foundation for the prevention and control of mastiffs in dairy cows. [ Method] Ten strains were isolated from milk samples produced by diseased dairy cows suffering from mastitis for morphologic observation, culture characteristic investigation, biochemical identification and Lancefield grouping. The isolated strains were identified at the molecular level by nested-PCR. [ Result] Among the ten isolates, six strains were 13-hemolytic and Gram-positive on blood agar, belonging to Lancefield group B, which were identified as streptococcus agalactiae by biochemical identification and nested-PCR. After overnight incubation, the coincidence rate between results of nested-PCR detection and biochemical identification reached 100%. [ Conclusion ] Bacterial incubation, rapid DNA extraction and specific PCR can provide basis for early epidemiological survey of streptococcus agalactiae infection in cattle.

关键词： Mastitis streptococcus agalactiae PCR 16S rRNA

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Antibacterial Activity of Herbal Preparations against Staphylococcus aureus and streptococcus agalactiae in Cow Mastitis

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Agricultural Science & Technology 2013年第9期14卷 1289-1290,1298页

作者：高桂生高光平李正本史秋梅张艳英邵新华梁银聚河北省预防兽医学重点实验室河北科技师范学院动物科技学院河北昌黎066600 迁安市金福祉奶牛养殖场河北迁安064400 河北新华科极兽药有限公司河北栾城051430

[Objective] This study aimed to analyze the antibacterial activity of herbal preparations against Staphylococcus aureus and streptococcus agalactiae in cow mastitis. [Method] The crude drug solutions of four different prescriptions for Zengrujianniusan were prepared through reflux extraction. Their antibacterial activity in vitro against Staphylococcus aureus and streptococcus agalactiae in cow mastitis were investigated. [Result] All the four different prescriptions exhibited antibacterial activity against S. aureus and S. agalactiae. Among them, prescription Ⅲ was extremely sensitive, and had the best bactericidal effect. The other three prescriptions were highly sensitive. [Conclusion] This study provides a theoretical basis for the development of herbal preparations for the treatment of cow mastitis.

关键词： Zengrujianniusan Staphylococcus aureus streptococcus agalactiae Antibacterial activity

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Comparative proteome analysis of two streptococcus agalactiae strains from cultured tilapia with different virulence

Comparative proteome analysis of two Streptococcus agalactia...

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2014年中国水产学会学术年会

作者： Wei Li You-Lu Su Yong-Zhan Mai Yan-Wei Li An-Xing Li The School of Life Sciences Sun Yat-sen University

streptococcus agalactiae is a major piscine pathogen, which causes significant morbidity and mortality among numerous fish species, and results in huge economic losses to aquaculture. Many S. agalactiae strains showing different virulence characteristics have been isolated from infected tilapia in different geographical regions throughout South China in the recent years, including natural attenuated S. agalactiae strain TFJ0901 and virulent S. agalactiae strain THN0901. In the present study, survival of tilapia challenged with S. agalactiae strain TFJ0901 and THN0901（107CFU/fish） were 93.3% and 13.3%, respectively. Moreover, there are severe lesions of the examined tissues in tilapia infected with strain THN0901, but no significant histopathological changes were observed in tilapia infected with the strain TFJ0901. In order to elucidate the factors responsible for the invasive potential of S. agalactiae between two strains TFJ0901 and THN0901, a comparative proteome analysis was applied to identify the different protein expression profiles between the two strains. 506 and 508 cellular protein spots of S. agalactiae TFJ0901 and THN0901 were separated by two dimensional electrophoresis, respectively. And 34 strain-specific spots, corresponding to 27 proteins, were identified successfully by MALDI-TOF mass spectrometry. Among them, 23 proteins presented exclusively in S. agalactiae TFJ0901 or THN0901, and the other 4 proteins presented in different isomeric forms between TFJ0901 and THN0901. Most of the strain-specific proteins were just involved in metabolic pathways, while 7 of them were presumed to be responsible for the virulence differences of S. agalactiae strain TFJ0901 and THN0901, including molecular chaperone Dna J, dihydrolipoamide dehydrogenase, thioredoxin, manganese-dependent inorganic pyrophosphatase, elongation factor Tu, bleomycin resistance protein and cell division protein Div IVA. These virulence-associated proteins may contribute to identify new di

关键词： streptococcus agalactiae comparative proteome virulence tilapia

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Molecular Cloning, expression and purification of immunogenic proteins Pgi and Spb1 of streptococcus agalactiae

Molecular Cloning, expression and purification of immunogeni...

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中国水产学会鱼病专业委员会2013年学术研讨会

作者： Xiao Xixi Li Anxing Key Laboratory for Aquatic Products Safety of Ministry of Education School of Life Sciences Sun Yat-sen University

streptococcus agalactiae,also known as Group B streptococcus(GBS),is a majorpathogen that causes serious infections in numerous species of freshwater and marine *** antigens have potentials to be ideal vaccines against GBS *** thisstudy,we selected 2 immunogenic proteins glucose-6-phosphate isomeras(Pgi)and PI-2bBackbone protein(Spb1)to test their potentials as *** we extracted *** DNA to amplify the ORF of pgi and spb1 by PCR,and clone to pET-28a(+)expressionvector by T4 ligase to construct recombination expression *** plasmids weretransformed to *** BL21(DH3)and induced to express by *** showed the MW ofrPgi was0 kDa,and rSpb1 was3 *** 16℃,0.2mM IPTG,both of them existed in thesupernatant and *** the following vaccine preparation,we chose the soluble partsto conduct purification on Ni-NTA *** proteins were desperately eluted with 250 mM and300mM imidazole,and ultra-filtered to remove *** concentration of rPgi and rSpb1were diluted to 1mg/ml in PBS and mixed as 1:1 emulsions in adjuvant to formulate *** task is to test the protection rate offered by the proteins in Nile tilapia against ***.

关键词： streptococcus agalactiae Pgi Spb1 vaccine

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