PCR Detection of Streptococcus agalactiae in Dairy Cows Suffering from Mastitis

作     者：Yanying ZHANG Guisheng GAO Zhengben LI Guangping GAO Qiumei SHI Xinhua SHAO Yinju LIANG Huiran LIU 

作者机构：Key Laboratory of Preventive Veterinary Medicine of Hebei ProvinceCollege of Animal Science and TechnologyHebei Normal University of Science and Technology Jinfuzhi Dairy Farm of Qian'an City Hebei Xinhua Animal Drug Co.Ltd. 

出 版 物：《Agricultural Biotechnology》 (农业生物技术（英文版）)

年 卷 期：2013年第2卷第5期

页      面：22-24页

学科分类：090603[农学-临床兽医学] 09[农学] 0906[农学-兽医学] 

基　　金：Supported by Science and Technology Development Project of Shijiazhuang City(08150132A) Spark Program of the Ministry of Science and Technology of China(2012GA6200025) Scientific Research Innovation Team of Hebei Normal University of Science and Technology(CXTD201201) Natural science Foundation of China(31072004) 

主　　题：Mastitis Streptococcus agalactiae PCR 16S rRNA 

摘      要：[ Objective ] This study aimed to identify Streptococcus agalactiae and lay the foundation for the prevention and control of mastiffs in dairy cows. [ Method] Ten strains were isolated from milk samples produced by diseased dairy cows suffering from mastitis for morphologic observation, culture characteristic investigation, biochemical identification and Lancefield grouping. The isolated strains were identified at the molecular level by nested-PCR. [ Result] Among the ten isolates, six strains were 13-hemolytic and Gram-positive on blood agar, belonging to Lancefield group B, which were identified as Streptococcus agalactiae by biochemical identification and nested-PCR. After overnight incubation, the coincidence rate between results of nested-PCR detection and biochemical identification reached 100%. [ Conclusion ] Bacterial incubation, rapid DNA extraction and specific PCR can provide basis for early epidemiological survey of Streptococcus agalactiae infection in cattle.