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Expression of Key steroidogenic enzymes in Human Placenta and Associated Adverse Pregnancy Outcomes

Maternal-Fetal Medicine 2023年第3期5卷 163-172页

作者： Jiasong Cao Yixin Wang Shuqi Wang Yongmei Shen Wen Li Zhuo Weil Shanshan Li Qimei Lin Ying Chang Tianjin Key Laboratory of Human Development and Reproductive Regulation Tianjin Central Hospital of Gynecology Obstetrics and Nankai University Affiliated Hospital of Obstetrics and GynecologyTianjin 300100China School of Clinical Medicine Tianjin Medical UniversityTianjin 300070China

Steroid hormones,including progestagens,estrogens,androgens,corticosteroids,and their precursor cholesterol,perform essential functions in the successful establishment and maintenance of pregnancy and normal fetal *** the core endocrine organ at the prenatal stage,the human placenta is involved in the biosynthesis,metabolism,and delivery of steroid *** pathways are tightly regulated by placenta-intrinsic cytochrome P450 and hydroxysteroid ***,the relationship between placental steroidogenic enzyme expression and adverse pregnancy outcomes is *** this review,we summarize the possible upstream regulatory mechanisms of placental steroidogenic enzymes in physiologic and pathophysiologic *** also describe the human placental barrier model and examine the potential of single-cell sequencing for evaluating the primary functions and cellular origin of steroidogenic ***,we examine the existing evidence for the association between placental steroidogenic enzyme dysregulation and adverse pregnancy outcomes.

关键词： Placenta steroidogenic enzymes Maternal-fetal outcomes Cytochrome P450 Hydroxysteroid dehydrogenase

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Changes in neurosteroid levels and steroidogenic enzyme expression in the brain of morphine dependent and withdrawing rats

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Neural Regeneration Research 2010年第7期5卷 514-518页

作者： Caizhen Yan Xiuli Wang Ping Jiang Yanning Hou Department of Pharmacology Hebei Medical University Shijiazhuang 050017 Hebei Province China Department of Anesthesiology Third Hospital of Hebei Medical University Shijiazhuang 050051 Hebei Province China Department of Pharmacology Beathune International Peace Hospital Shijiazhuang 050082 Hebei Province China

BACKGROUND： Studies have demonstrated that exogenous neurosteroid treatment prevents the development of morphine tolerance and dependence, and attenuates abstinence behavior in mice. However, there are few studies on whether the levels of endogenous neurosteroids can be changed by morphine dependence and withdrawal. OBJECTIVE： To investigate the levels of various neurosteroids in rat brain following morphine dependence and withdrawal. To evaluate the expressions of steroidogenic enzyme mRNAs and proteins. To identify the relationship between neurosteroids and morphine dependence at the whole animal behavior, neural biochemistry, and molecular levels. DESIGN, TIME AND SETTING： A randomized, controlled study. Experiments were performed at the Department of Pharmacology of Hebei Medical University and Department of Pharmacology of Beathune International Peace Hospital, China, from June 2004 to October 2007. MATERIALS： Morphine hydrochloride injection （Shenyang First Pharmaceutical Factory, China）, naloxone hydrochloride （Hunan Yiqiao Pharmaceutical Co., China） and a gas chromatography-mass spectrometry system （Agilent, CA, USA） were used in this study. METHODS： Healthy adult Sprague Dawley rats were randomly divided into three groups： a morphine dependence group, morphine withdrawal group and control group （n = 20）. The rats in the morphine dependence and morphine withdrawal groups were given increasing doses of morphine （5, 10, 15, 20, 30, 40 and 50 mg/kg, intraperitoneal） to create morphine dependence. The rats in the morphine withdrawal group were injected with 2 mg/kg naloxone to precipitate withdrawal 1 hour after the last morphine injection. Rats in the control group were treated with an equal volume of saline. MAIN OUTCOME MEASURES： Following morphine dependence and withdrawal, brain levels of the neurosteroids pregnenolone, progesterone and allopregnanolone were analyzed using gas chromatography-mass spectrometry. The mRNA expression of two key steroidogenic en

关键词： morphine dependence withdrawal neurosteroids steroidogenic enzymes gas chromatography-mass spectrometry neural regeneration

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Acephate interferes with androgen synthesis in rat immature Leydig cells in vitro

Acephate interferes with androgen synthesis in rat immature ...

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中国药理学会第十一届全国基础与临床生殖药理学术研讨会暨...

作者： Tongliang Huang Yayoyao Dong Siwen Wu Qiqi Zhu Xiaoheng Li Shiwen Liu Huitao Li Ren-Shan Ge Department of Anesthesiology The Second Affiliated Hospital and Yuying Children's HospitalWenzhou Medical University Department of Pharmacy The Second Affiliated Hospital and Yuying Children's Hospital Wenzhou Medical University

OBJECTIVE: Acephate is a widely used organophosphorus insecticide, but whether it inhibits androgen synthesis and metabolism remains unclear. In the current study, we investigated acephate in the inhibition of androgen synthetic and metabolic pathways in rat immature Leydig cells. METHODS: After 3 h culture in vitro with different concentrations（0, 0.5, 5,and 50 m M） of acephate, immature Leydig cell androgen synthesis related and other genes expression tested by qPCR and Western blot. RESULTS: Acephate inhibited basal androgen output in a dose-dependent manner with the inhibition starting at 0.5 m M. It significantly inhibited luteinizing hormone and 8-Br-cAMP stimulated androgen production at 50 m M. It significantly inhibited progesterone-mediated androgen output at 50 mM. Further study found that acephate down-regulated the expression of Hsd3b1 at 0.5 mM, Lhcgr at 5 mM and Star at 50 mM. Acephate directly blocked rat testicular HSD3B1 activity at 50 m M. Acephate did not affect other androgen synthetic and metabolic enzyme activities as well as ROS production, proliferation, and apoptosis of Leydig cells. CONCLUSION: Acephate targets LHCGR, STAR, and HSD3B1, thus blocking androgen synthesis in rat immature Leydig cells and HSD3B1 is the most sensitive target of acephate.

关键词： Steroidogenesis Acephate Rat immature Leydig cells steroidogenic enzymes

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