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2014年第九次全国牙体牙髓病学学术会议

作者： Yao Li Yan Yu Shu Ma Jinhua Yu Jiangsu Key Laboratory of Oral Diseases Nanjing Medical University Department of Endodontics School of StomatologyNanjing Medical University

Objective:Estrogen plays an important role in the osteogenic differentiation of mesenchymal stem cells while stem cells from apical papilla(SCAPs) can contribute to the formation of dentin/bone-like tissues.To date,the effects of estrogen on the differentiation of SCAPs remain unclear.Methods:SCAPs were isolated and treated with 10-7 M 17beta-estradiol(E2).Their odonto/osteogenic potency and the involvement of mitogen-activated protein kinase(MAPK) signaling pathway were subsequently investigated by using methylthiazolyl-tetrazolium(MTT) assay,etc.Results:MTT and flow cytometry results demonstrated that E2 treatment had no effect on the proliferation of SCAPs in vitro,while alkaline phosphatase(ALP) assay and alizarin red staining showed that E2 could significantly promotes ALP activity and mineralization ability in SCAPs.Real-time reverse transcription polymerase chain reaction(RT-PCR) and western blot assay revealed that the odonto/osteogenic markers(ALP,DMP1/DMP1,DSPP/DSP,RUNX2/RUNX2,OSX/OSX and OCN/OCN) were significantly up-regulated in E2-treated SCAPs.In addition,the expression of phosphor-p38 and phosphor-JNK in these stem cells was enhanced by E2 treatment,so was the expression of the nuclear downstream transcription factors including phosphor-Sp1,phosphor-Elk-1,phosphor-c-Jun and phosphor-c-Fos,indicating the activation of MAPK signaling pathway during the odonto/osteogenicdifferentiation of E2-treated SCAPs.Conversely,the differentiation of E2-treated SCAPs was inhibited in the presence of MAPK specific inhibitors.Conclusion:10-7 M 17beta-estradiol enhanced the odonto/osteogenic differentiation of SCAPs via the activation of MAPK signaling pathway.

关键词： 17beta-estradiol stem cells from apical papilla Odonto/osteogenic differentiation MAPK signaling pathway

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Extracellular vesicles derived from human dental mesenchymal stem cells stimulated with low-intensity pulsed ultrasound alleviate inflammation-induced bone loss in a mouse model of periodontitis

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Genes & Diseases 2023年第4期10卷 1613-1625页

作者： Tingwei Zhang Ziqi Chen Mengyuan Zhu Xuan Jing Xiaohui Xu Xulei Yuan Mengjiao Zhou Yanan Zhang Miao Lu Duanjing Chen Shihan Xu Jinlin Song Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences Chongqing Municipal Key Laboratory for Oral Biomedical Engineering of Higher Educationand Stomatological Hospital of Chongqing Medical UniversityChongqing 401147China

Extracellular vesicles(EVs)derived from mesenchymal stem cells(MSCs)have emerged as a new mode of intercellular crosstalk and are responsible for many of the thera-peutic effects of MSCs.To promote the application of MSC-EVs,recent studies have focused on the manipulation of MSCs to improve the production of EVs and EV-mediated activities.The current paper details an optimization method using non-invasive low-intensity pulsed ul-trasound(LIPUS)as the stimulation for improving oral MSC-EV production and effectiveness.stem cells from apical papilla(SCAP),a type of oral mesenchymal stem cell,displayed inten-sity-dependent pro-osteogenic and anti-inflammatory responses to LIPUS without significant cytotoxicity or apoptosis.The stimuli increased the secretion of EVs by promoting the expres-sion of neutral sphingomyelinases in SCAP.In addition,EVs from LIPUS-induced SCAP exhibited stronger efficacy in promoting the osteogenic differentiation and anti-inflammation of peri-odontal ligament cells in vitro and alleviating oral inflammatory bone loss in vivo.In addition,LIPUS stimulation affected the physical characteristics and miRNA cargo of SCAP-EVs.Further investigations indicated that miR-935 is an important mediator of the pro-osteogenic and anti-inflammatory capabilities of LIPUS-induced SCAP-EVs.Taken together,these findings demonstrate that LIPUS is a simple and effective physical method to optimize SCAP-EV produc-tion and efficacy.

关键词： Extracellular vesicles Low-intensity pulsed ultrasound Oral bone loss Periodontitis stem cells from apical papilla

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Oral stem cells,decoding and mapping the resident cells populations

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Biomaterials Translational 2022年第1期3卷 24-30页

作者： Xuechen Zhang Ana Justo Caetano Paul T.Sharpe Ana Angelova Volponi Centre for Craniofacial and Regenerative Biology Faculty of DentistryOral&Craniofacial SciencesKing’s College University of LondonLondonUK Laboratory of Odontogenesis and Osteogenesis Institute of Animal Physiology and GeneticsCASv.v.i.BrnoCzech Republic

The teeth and their supporting tissues provide an easily accessible source of oral stem cells.These different stem cell populations have been extensively studied for their properties,such as high plasticity and clonogenicity,expressing stem cell markers and potency for multilineage differentiation in vitro.Such cells with stem cell properties have been derived and characterised from the dental pulp tissue,the apical papilla region of roots in development,as well as the supporting tissue of periodontal ligament that anchors the tooth within the alveolar socket and the soft gingival tissue.Studying the dental pulp stem cell populations in a continuously growing mouse incisor model,as a traceable in vivo model,enables the researchers to study the properties,origin and behaviour of mesenchymal stem cells.On the other side,the oral mucosa with its remarkable scarless wound healing phenotype,offers a model to study a well-coordinated system of healing because of coordinated actions between epithelial,mesenchymal and immune cells populations.Although described as homogeneous cell populations following their in vitro expansion,the increasing application of approaches that allow tracing of individual cells over time,along with single-cell RNA-sequencing,reveal that different oral stem cells are indeed diverse populations and there is a highly organised map of cell populations according to their location in resident tissues,elucidating diverse stem cell niches within the oral tissues.This review covers the current knowledge of diverse oral stem cells,focusing on the new approaches in studying these cells.These approaches“decode”and“map”the resident cells populations of diverse oral tissues and contribute to a better understanding of the“stem cells niche architecture and interactions.Considering the high accessibility and simplicity in obtaining these diverse stem cells,the new findings offer potential in development of translational tissue engineering approaches and innovative therapeutic solutions.

关键词： dental pulp stem cells dental stem cells gingival stem cells periodontal ligament stem cells stem cells from apical papilla

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Application of dental stem cells in three-dimensional tissue regeneration

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World Journal of stem cells 2021年第11期13卷 1610-1624页

作者： Hui-Yi Hsiao Chung-Yi Nien Hsiang-Hsi Hong Ming-Huei Cheng Tzung-Hai Yen Center for Tissue Engineering Chang Gung Memorial HospitalLinkou BranchTaoyuan 333Taiwan Department of Life Sciences National Central UniversityZhongliTaoyuan 320Taiwan Department of Periodontics Chang Gung Memorial HospitalLinkou BranchTaoyuan 333Taiwan Division of Reconstructive Microsurgery Department of Plastic and Reconstructive SurgeryChang Gung Memorial HospitalLinkou BranchCollege of MedicineChang Gung UniversityTaoyuan 333Taiwan Department of Nephrology Clinical Poison CenterChang Gung Memorial HospitalLinkou BranchTaoyuan 333Taiwan School of Traditional Chinese Medicine College of MedicineChang Gung UniversityTaoyuan 333Taiwan

Dental stem cells can differentiate into different types of cells.Dental pulp stem cells,stem cells from human exfoliated deciduous teeth,periodontal ligament stem cells,stem cells from apical papilla,and dental follicle progenitor cells are five different types of dental stem cells that have been identified during different stages of tooth development.The availability of dental stem cells from discarded or removed teeth makes them promising candidates for tissue engineering.In recent years,three-dimensional(3D)tissue scaffolds have been used to reconstruct and restore different anatomical defects.With rapid advances in 3D tissue engineering,dental stem cells have been used in the regeneration of 3D engineered tissue.This review presents an overview of different types of dental stem cells used in 3D tissue regeneration,which are currently the most common type of stem cells used to treat human tissue conditions.

关键词： Dental stem cells Dental pulp stem cells stem cells from human exfoliated deciduous teeth Periodontal ligament stem cells stem cells from apical papilla Dental follicle progenitor cells Three-dimensional tissue regeneration

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