Extracellular vesicles derived from human dental mesenchymal stem cells stimulated with low-intensity pulsed ultrasound alleviate inflammation-induced bone loss in a mouse model of periodontitis

作     者：Tingwei Zhang Ziqi Chen Mengyuan Zhu Xuan Jing Xiaohui Xu Xulei Yuan Mengjiao Zhou Yanan Zhang Miao Lu Duanjing Chen Shihan Xu Jinlin Song Tingwei Zhang;Ziqi Chen;Mengyuan Zhu;Xuan Jing;Xiaohui Xu;Xulei Yuan;Mengjiao Zhou;Yanan Zhang;Miao Lu;Duanjing Chen;Shihan Xu;Jinlin Song

作者机构：Chongqing Key Laboratory for Oral Diseases and Biomedical SciencesChongqing Municipal Key Laboratory for Oral Biomedical Engineering of Higher Educationand Stomatological Hospital of Chongqing Medical UniversityChongqing 401147China 

出 版 物：《Genes & Diseases》 (基因与疾病（英文）)

年 卷 期：2023年第10卷第4期

页      面：1613-1625页

核心收录：

学科分类：1003[医学-口腔医学] 100302[医学-口腔临床医学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China(No.81771082,81800985,82170968,31971282) the 2019 Chongqing Graduate Tutor Team Construction Project(No.dstd201903),China the Natural Science Foundation of Chongqing(No.cstc2019jcyj-msxmX0851),China 

主　　题：Extracellular vesicles Low-intensity pulsed ultrasound Oral bone loss Periodontitis Stem cells from apical papilla 

摘      要：Extracellular vesicles(EVs)derived from mesenchymal stem cells(MSCs)have emerged as a new mode of intercellular crosstalk and are responsible for many of the thera-peutic effects of *** promote the application of MSC-EVs,recent studies have focused on the manipulation of MSCs to improve the production of EVs and EV-mediated *** current paper details an optimization method using non-invasive low-intensity pulsed ul-trasound(LIPUS)as the stimulation for improving oral MSC-EV production and *** cells from apical papilla(SCAP),a type of oral mesenchymal stem cell,displayed inten-sity-dependent pro-osteogenic and anti-inflammatory responses to LIPUS without significant cytotoxicity or *** stimuli increased the secretion of EVs by promoting the expres-sion of neutral sphingomyelinases in *** addition,EVs from LIPUS-induced SCAP exhibited stronger efficacy in promoting the osteogenic differentiation and anti-inflammation of peri-odontal ligament cells in vitro and alleviating oral inflammatory bone loss in *** addition,LIPUS stimulation affected the physical characteristics and miRNA cargo of *** investigations indicated that miR-935 is an important mediator of the pro-osteogenic and anti-inflammatory capabilities of LIPUS-induced *** together,these findings demonstrate that LIPUS is a simple and effective physical method to optimize SCAP-EV produc-tion and efficacy.