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Virologica Sinica 2016年第5期31卷 425-436页

作者： Zheng Fang Jingxu Shao Qingbei Weng School of Life Sciences Guizhou Normal University

Cells of the P8-Se301-C1 strain are spodoptera exigua cell clones that each harbor a partial version of the S. exigua multiple nucleopolyhedrovirus （SeMNPV） genome and which are resistant to homologous SeMNPV infections. The cells produce no viral progeny, suggesting that the infection is a latent-like viral infection. To investigate the SeMNPV genes harbored in the P8-Se301-C1 cells, the de novo transcriptomes of P8-Se301-Cl cells and S. exigua Se301 cells were analyzed and compared. A total of 54,569,296 reads were obtained from the P8-Se301-C1 cells that yielded 112,565 final unigenes with a mean length of 1,093 nt. A total of 56,865,504 reads were obtained from the Se301 cells that yielded 102,996 final unigenes with a mean length of 1,082 nt. Ten SeMNPV gene transcripts （se5, se7, se8, se12, se43, se45, se89, se90, se124, and se126） were detected in the P8-Se301-C1 cells by RNA-Seq but not in the Se301 cells, which was verified by RT- PCR. 5＇/3＇ RACE analyses showed that the 3＇- or 5＇-end sequences of the viral transcripts are aligned to the host gene sequences in P8-Se301-C1 cells, suggesting that the SeMNPV genes may integrate into and be transcribed with the host genes in the P8-Se301-C1 cells. Furthermore, six additional viral gene transcripts, sell, se42, se44, se88, se91, and se127 （incorporated into chimeric fusion transcripts in the P8-Se301-C1 cells）, were detected in the RACE analyses. Taken together, sixteen SeMNPV transcripts were identified in the P8-Se301-C1 cell strain. This study provides information to develop the understanding of baculovirus latent infections and superinfection exclusion.

关键词： RNA-Seq SeMNPV baculovirus latent infection spodoptera exigua

Tissue distribution of cadmium and its effect on reproduction in spodoptera exigua

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Journal of Integrative Agriculture 2024年第1期23卷 195-204页

作者： Honghua Su Menglu Wu Yong Yang Yan Deng Yizhong Yang Qingming Sun College of Plant Protection Yangzhou UniversityYangzhou 225009China Institute of Fruit Tree Research Guangdong Academy of Agricultural Sciences/Key Laboratory of South Subtropical Fruit Biology and Genetic Resource UtilizationMinistry of Agriculture and Rural Affairs/Guangdong Provincial Key Laboratory of Tropical and Subtropical Fruit Tree ResearchGuangzhou 510640China

Vegetable fields are often contaminated by heavy metals,and spodoptera exigua is a major vegetable pest which is stressed by heavy metals mainly by *** this study,cadmium accumulation in the tissues of *** exposed to cadmium and its effects on the growth and development of the parents and the offspring were *** the stress of different concentrations of cadmium(0.2,3.2,and 51.2 mg kg^(-1)),the cadmium content in each tissue of *** increased in a dose-dependent *** the larval stage,the highest cadmium accumulation was found in midgut in all three cadmium treatments,but at the adult stage,the highest cadmium content was found in fat *** addition,the cadmium content in ovaries was much higher than in *** *** was stressed by cadmium and the F_(2)generation was not fed a cadmium-containing diet,the larval survival,pupation rate,emergence rate and fecundity of the F_(2)generation were significantly reduced in the 51.2 mg kg^(-1)treatment compared to the corresponding *** in the F_(2)generation of the 3.2 mg kg^(-1)treatment,the fecundity was significantly lower than in the parental *** fecundity of the only-female stressed treatment was significantly lower than that of the only-male stressed treatment at the 3.2 and 51.2 mg kg^(-1)cadmium exposure *** only mothers were stressed at the larval stage,the fecundity of the F_(2)generation was significantly lower than that of the F1generation in the 51.2 mg kg^(-1)treatment,and it was also significantly lower than in the 3.2 and 0.2 mg kg^(-1)*** results of our study can provide useful information for forecasting the population increase trends under different heavy metal stress conditions and for the reliable environmental risk assessment of heavy metal pollution.

关键词： heavy metal pollution cadmium spodoptera exigua tissue distribution reproduction

Induced CTL-S15 gene expression by Bacillus thuringiensis declines susceptibility in spodoptera exigua

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Journal of Integrative Agriculture 2024年第9期23卷 3078-3088页

作者： Jianqiang Bao Yuxuan Chen Suwan Jiang Rui Liu Xi Zhang Fangzheng Zhang Zhiwei Chen Chen Luo Hailong Kong College of Plant Protection Yangzhou UniversityYangzhou 225009China

It has been reported that C-type lectins(CTLs),which are pattern recognition receptors of the insect innate immunity response,may compete with Cry toxin for the receptor alkaline phosphatase to decrease its toxicity in ***,to date,which CTLs affect larval susceptibility to Bt in spodoptera exigua is not *** this study,33 CTL genes were identified from *** on the number of carbohydrate-recognition domains(CRDs)and the domain architectures,they were classified into three groups:(1)nineteen CTL-S(single-CRD),(2)eight immulectin(dual-CRD)and(3)six CTL-X(CRD with other domains).RT-qPCR analysis revealed that expression levels of SeCTL-S15,IML-4 and CTL-X6 were upregulated after challenge with Bt and *** and developmental stage expression analysis showed that only SeCTL-S15 was mainly expressed in the midgut and larva,*** of SeCTL-S15 significantly increased Bt susceptibility,as indicated by reduced survival and larval *** results suggest that CTL-S15 might play a vital role in the low susceptibility of larvae to Bt in *** results provide new insights into CTL function in insects.

关键词： spodoptera exigua Bacillus thuringiensis susceptibility C-type lectins

Effect of Host Plants on Development,Fecundity and Enzyme Activity of spodoptera exigua (Hübner)(Lepidoptera:Noctuidae)

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Agricultural Sciences in China 2011年第8期10卷 1232-1240页

作者： ZHANG Bin LIU Huai Helen Hull-Sanders WANG Jin-jun Chongqing Key Laboratory of Entomology and Pest Control Engineering Southwest UniversityChongqing 400716P.R.China Chongqing Tobacco Company China National Tobacco CorporationChongqing 400023P.R.China

The beet armyworm,spodoptera exigua （Lepidoptera：Noctuidae）,is an economically important pest of crops worldwide,attacking plants from over 20 families including trans-continental agricultural cotton,corn and citrus *** this study,performance and subsequent enzyme activity of beet armyworm reared on host plants from five families were *** differences were found in development,fecundity and enzyme activity on different host *** rate was the highest （42.8%） on asparagus lettuce （Lactuca sativar ***） and the lowest （17.0%） on sweet pepper （Capsicum annuum）.Larval duration was the shortest on asparagus lettuce （12.0 d）,and was 43.4% longer on sweet peppers （21.2 d）.The activity of acetylcholine esterase （AChE） and carboxylesterase （CarE） in 3rd instar larvae,and soluble carbohydrate and crude protein contents in different host plants were *** activity was the highest in the larvae feeding on Chinese cabbage （Brassica rapa）,but declined by nearly 60% on maize （Zea mays） *** ratio of soluble carbohydrate content to crude protein content in host plants was found to have a positive effect on oviposition and a negative correlation with larval duration and life time （from larval to adult stages） of the insect.

关键词： beet armyworm spodoptera exigua host plants nutrition enzyme activity

Three metabolites from an entomopathogenic bacterium, Xenorhabdus nematophila, inhibit larval development of spodoptera exigua （Lepidoptera： Noctuidae） by inhibiting a digestive enzyme, phospholipase A2

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Insect Science 2011年第3期18卷 282-288页

作者： Jaehyun Kim Yonggyun Kim Department of Bioresource Sciences Andong National University Andong Korea

An entomopathogenic bacterium, Xenorhabdus nematophila, has been known to induce significant immunosuppression of target insects by inhibiting immune-associated phospholipase A2 （PLA2）, which subsequently shuts down biosynthesis of eicosanoids that are critical in immune mediation in insects. Some metabolites originated from the bacterial culture broth have been identified and include benzylideneacetone, proline-tyrosine and acetylated phenylalanine-glycine-valine, which are known to inhibit enzyme activity of PLA2 extracted from hemocyte and fat body. This study tested their effects on digestive PLA2 of the beet armyworm, spodoptera exigua. Young larvae fed different concentrations of the three metabolites resulted in significant adverse effects on larval development even at doses below 100 #g/mL. In particular, they induced significant reduction in digestive efficiency of ingested food. All three metabolites significantly inhibited catalytic activity of digestive PLA2 extracted from midgut lumen of the fifth instar larvae at a low micromolar range. These results suggest that the inhibitory activities of the three bacterial metabolites on digestive PLA2 of S. exigua midgut may explain some of their oral toxic effects.

关键词： digestion midgut phospholipase A2 spodoptera exigua Xenorhabdus nematophila

Antiviral function of peptidoglycan recognition protein in spodoptera exigua(Lepidoptera:Noctuidae)

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Insect Science 2023年第4期30卷 1092-1104页

作者： Jie Li Jie Li Zhaohao Jing Qianlong Yu Guiling Zheng Bin Zhang Longsheng Xing Huan Zhang Fanghao Wan Changyou Li Shandong Engineering Research Center for Environment-Friendly Agricultural Pest Management Cllege of Plant Health and MedicineQingdao Agricultural UniversityQingdaoChina Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural SciencesShenzhenChina Institute of Zoology Chinese Academy of SciencesBejingChina

Peptidoglycan recognition proteins(PGRPs)are a class of molecules that play a critical role in insect *** the function of PGRPs is important to improve the efficiency of microbial *** this study,we investigated the role of PGRP-LB(a long type PGRP)in insect immunity against viruses using spodoptera exigua and spodoptera exigua multiple nucleopolyhedrovirus(SeMNPV)as an insect-virus *** cloned and identified a PGRP-LB gene from ***;the gene consisted of 7 exons that encoded a polypeptide of 234 amino acids with a signal peptide and a typical amidase *** analysis revealed that the abundance of SePGRP-LB transcripts in the fat body was greater than in other *** of SePGRP-LB resulted in a significant decrease of 49%in the rate of SeMNPV-infected *** addition,the multiplication of SeMNPV was significantly decreased:a decrease of 79%in the production of occlusion-derived virion(ODV),and a maximum decrease of 50%in the production of budded virion(BV).In contrast,silencing of SePGRP-LB expression by RNA interference resulted in a significant 1.65-fold increase in the rate of SeMNPV-infected cells,a significant 0.54-fold increase in ODV production,a maximum 1.57-fold increase in BV production,and the larval survival dropped to 21%.Our findings show that SePGRP-LB has an antiviral function against SeMNPV,and therefore this gene may provide a target for lepidopteran pest control using virus insecticides.

关键词： antiviral long-type peptidoglycan recognition protein gene PGRP-LB spodoptera exigua multiple nucleopolyhedrovirus spodoptera exigua

Identification of four caspase genes from spodoptera exigua (Lepidoptera: Noctuidae) and their regulations toward different apoptotic stimulations

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Insect Science 2020年第6期27卷 1158-1172页

作者： Huan Yu Zi-Qi Li Yi-Yi Ou-Yang Guo-Hua Huang Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests Hunan Agricultural UniversityChangshaChina College of Plant Protection Hunan Agricultural UniversityChangshaChina

Apoptosis plays critical roles in multiple biological processes in multicellular *** are known as important participators and regulators of ***,four novel caspase genes of spodoptera exigua were cloned and characterized which were designated as SeCasp-1,SeCasp-6,ScCasp-7 and *** of the putative encoded protein sequences of these SeCasps indicated that SeCasp-1 and ScCasp-7 were possible homologs of executor caspases;SeCasp-8 was a possible homolog of initiator caspases;and SeCasp-6 was a unique caspase of *** that shares low similarity with all the identified insect *** on baculovirus expression system analyses,SeCasp-1 exhibited similar caspase activity to human caspase-1,-3,-4,-6,-8 and-9;SeCasp-6 presented similar caspase activity to human caspase-2,-3,-4,-6,-8 and-9;SeCasp-7 exhibited similar caspase activity to human caspase-2,-3 and-6;and SeCasp-8 presented similar caspase activity only to human *** with diiTcrent chemicals revealed that SeCasp-1 showed extreme upregulation after 24 h in the treated fat body cell line(IOZCAS-Spex-II)of *** expression analysis revealed that SeCasp-1 was highly transcribed in the larval stages,while SeCasp-6,SeCasp-7,SeCasp-H were *** in vivo detection of the relative expression levels of SeCasps in *** larvae inoculated with different pathogens suggested that SeCasp-J was sensitive to Bacillus thuringiensis infection and that SeCasp-6 was sensitive to baculovirus ***-7 and SeCasp-H showed slight changes under either in vitro chemical apoptosis induction or in vivo pathogen infection.

关键词： apoptosis apoptotic inducing insect caspase SeCasp spodoptera exigua

A Fragment of Cadherin-Like Protein Enhances Bacillus thuringiensis Cry1B and Cry1C Toxicity to spodoptera exigua(Lepidoptera:Noctuidae)

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Journal of Integrative Agriculture 2012年第4期11卷 628-638页

作者： LU Qiong ZHANG Yongojun CAO Guang-chun ZHANG Li-li LIANG Ge-mei LU Yan-hui WU Kong-ming GAO Xi-wu GUO Yu-yuan College of Agriculture and Biotechnology China Agricultural University Beijing 100193 P.R.China State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing 100193 P.R. China

Cry toxins produced by Bacillus thuringiensis（Bt） are effective biological insecticides against certain insect *** this study,bioassay results indicated that Cry1B and Cry1C were toxic to spodoptera *** also identified a cadherin-like gene in *** that could enhance the toxicity of Cry1B and *** cadherin-like gene identified from the larvae midgut tissue was cloned by reverse transcription polymerase chain reaction（RT-PCR） and rapid amplification of cDNA ends（RACE）.The full-length cDNA of the gene consisted of 5 220 bp encoding 1 740 amino acid with a predicted molecular mass of 196 *** search analysis showed that the predicted amino acid sequence had a high sequence identity to the published sequences of cadherin-like proteins from other Lepidoptera *** expression of the cadherin-like gene detected by qRT-PCR analysis revealed that the cadherin-like gene was mainly present in the gut of 4th instar larvae and during different life *** results suggested that the commercial development of this synergist has the potential to enhance Cry1B and Cry1C toxicity against Lepidoptera insects.

关键词： Bacillus thuringiensis cadherin-like spodoptera exigua enhancement toxicity

Temporal allocation of metabolic tolerance to transgenic Bt cotton in beet armyworm,spodoptera exigua(Hübner)

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Science China(Life Sciences) 2011年第2期54卷 152-158页

作者： GUO JianYing WU Gang WAN FangHao State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing 100193 China College of Plant Sciences and Technology Huazhong Agricultural University Hubei 430070 China

Digestive and detoxification enzyme activity and nutrient composition were examined in the body of fourth instar beet armyworms,spodoptera exigua(Hübner),fed on transgenic Bacillus thuringiensis(Bt) and non-Bt cotton for different time *** composition and specific enzyme activities differed significantly between the *** fed Bt ***-Bt *** 1,6 and 24 h,free fatty acid and glucose levels were significantly lower in *** fed on Bt cotton than those fed on non-Bt *** fed on Bt cotton had significantly higher trypsin and total superoxide dismutase(T-SOD) activities and significantly lower lipase,carboxylesterase and acetylcholinesterase activities than non-Bt fed worms for all feeding time *** were also observed among feeding times within each cotton variety *** lower free fatty acid and total amino acid were observed in *** fed on Bt cotton for 24 h than in those fed for 1 *** lower activities of lipase and trypsin were detected in *** fed on Bt cotton for 24 h than those for 1 and 4 ***,carboxylesterase and acetylcholinesterase activities in *** fed on Bt cotton for 24 h were significantly higher than those for 1,4 and 6 *** interaction between cotton variety and feeding time significantly affected the activities of lipase,trypsin,acetylcholinesterase and T-SOD enzymes in *** the temporal allocation of protection and detoxification enzyme activities in the body of *** in response to *** can provide a meaningful evaluation on the metabolic tolerance of herbivorous insects under the continuous selection pressure of a toxic protein.

关键词： Bacillus thuringiensis spodoptera exigua detoxification enzyme carboxylesterase acetylcholinesterase

Cloning and Sequencing of a Gene Encoding GOBP2 in the Antenna of spodoptera exigua

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Agricultural Sciences in China 2002年第2期1卷 200-206页

作者： WANG Gui-rong, GUO Yu-yuan, XU Guang and WU Kong-ming( The Institute of Plant Protection , Chinese Academy of Agricultural Sciences , Beijing 100094 ) The Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing 100094

A pair of degenerate primers was designed, based on the comparison of five insects' GOBP2 gene sequences reported previously. A specific band (about 400bp in length) was amplified from cDNA of spodoptera exigua antenna and another specific band (about 2kb in length) was amplified from genomic DNA. The two segments were cloned into T-easy vector, respectively. Results of sequencing and structural analysis showed that the full-length of GOBP2Sexi ORF is 426bp, 141 amino acid residues were encoded. The predicted MW and pI are 16.07ku and 5.09 .respectively. There are six conservative Cys locus in the sequence, which is the typical characteristic of OBPs. GOBP2Sexi gene was inserted by two introns between amino acid residue 22 and 23 and between 82 and 83. The length of two introns is 160bp and 1403bp. Results of Northern blot showed that GOBP2 gene expressed specifically in the antenna of spodoptera exigua, and the expression level is nearly equal in the antenna of male and female moths. The sequence was deposited in GenBank/EMBL and the accession number is AJ294809.

关键词： spodoptera exigua Antenna GOBP2 Gene cloning