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DNA methylation and alternative splicing modulate FBXW11 gene expression in Holstein bull testis and are correlated with sperm quality

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BIOCELL 2021年第1期45卷 79-87页

作者： YONG LIU ZHIHUA JU QIANG JIANG WENHAO LIU CHUNHONG YANG YARAN ZHANG XIUGE WANG YAPING GAO XIAOCHAO WEI YAN SUN JINPENG WANG MINGHAI HOU LING YANG JINMING HUANG Dairy Cattle Research Center Shandong Academy of Agricultural SciencesJinan250100China College of Life Sciences and Food Engineering Hebei University of EngineeringHandan056021China

F-box and WD-40 domain protein 11(FBXW11)is an important component of the E3 ubiquitin-ligase enzyme that plays a key role in the ubiquitin-dependent regulation of *** our previous research,the mRNA expression of FBXW11 in bull sperm with high motility is significantly higher than that with low *** the present study,the protein expression levels of FBXW11 in bull testicular tissues with low-performance sperm quality groups were significantly higher than those in normal performance *** immunohistochemistry result demonstrated that FBXW11 protein was located in the periphery of Leydig cells and seminiferous *** splice variants of the FBXW11 gene,namely,FBXW11-tv1,FBXW11-tv2,and FBXW11-tv3,were identified in testicular *** splicing patterns of the three variants are exon *** transcript FBXW11-tv2 expressions were the highest in each *** low-performance groups displayed higher FBXW11-tv1 and FBXW11-tv2 transcript expressions than the normal performance *** CpG islands were located within the 5’UTR and exon 1-2 region of the FBXW11 *** sequencing PCR results demonstrated that the methylation levels of 11 methylation sites in the CpG island 2 from−99 to−43 in the normal performance groups were significantly lower than those in the low-performance *** correlation analysis suggested that the CpG island 2 methylation level was negatively correlated with sperm motility and the transcript FBXW11-tv2 expression *** data revealed that alternative splicing and DNA methylation jointly regulated FBXW11 gene expression and were correlated with sperm quality traits during spermatogenesis in Holsteins.

关键词： FBXW11 Alternative splicing DNA methylation Bull sperm quality traits

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