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Expression analysis of OsSERK,OsLEC1 and OsWOX4 genes in rice(Oryza sativa L.)callus during somatic embryo development

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BIOCELL 2022年第7期46卷 1633-1641页

作者： SITI NABILAH TRI HANDOYO KYUNG-MIN KIM MOHAMMAD UBAIDILLAH Graduate School of Biotechnology University of JemberJember68121Indonesia Faculty of Agriculture University of JemberJember68121Indonesia Center Development of Advanced Science and Technology(CDAST) University of JemberJember68121Indonesia Division of Plant Biosciences School of Applied BioSciencesCollege of Agriculture and Life ScienceKyungpook National UniversityDaegu41566South Korea

somatic embryogenesis is an asexual reproduction process that occurs in many plant species,including *** process contains several totipotency markers such as somatic Embryogenesis Receptor-like Kinase(SERK),Leafy Cotyledon1(LEC1)and WUSCHEL-Related Homeobox4(WOX4)and also a helpful model for embryo development and clones and ***,we report the gene expression during somatic embryo development correlates with regeneration frequency in 14 Javanica rice(pigmented and non-pigmented)using modified N6 media supplemented with Kinetin(2.0 mg/L)and NAA(1.0 mg/L).Although there have been advances in understanding the genetic basis of somatic embryogenesis in other varieties,rice is still unexplored,especially during somatic embryo ***,for the formation of callus induction from immature embryos,2,4-D(2.0 mg/L,3.0 mg/L)was *** study analysed the gene expression of OsSERK,OsWOX4 and OsLEC1 genes through RT-PCR *** expression of the OsLEC1 gene indicates that their function may correlate in the in vitro with the high response of rice after transfer to regeneration *** study found that rice varieties of pigmented rice(MS Pendek and Gogoniti II)and non-pigmented rice(Pandan Ungu)showed high regeneration frequency,showing higher OsLEC1 expression than other varieties because OsLEC1 promotes the maturation of somatic embryos in plant regeneration on day ***,the contrast with Genjah nganjuk may be effective because of other regulatory ***-PCR analysis showed OsSERK had less expression level than OsLEC1 and OsWOX4 in the varieties,which correlate with the percentage of plant regeneration,but not for Gogoniti *** conclusion,the higher percentage of plant regeneration correlates with the higher expression level of OsLEC1 at day 14 of media regeneration of rice.

关键词： Javanica rice OsLEC1 OsSERK OsWOX4 Plant regeneration somatic embryos

Morphology of somatic embryogenesis and plantlet formation in tissue cultures of lantern tree (Koelreuteria bipinnata ***)

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Forestry Studies in China 2010年第1期12卷 31-36页

作者： FENG Da-ling MENG Qing-rong LI Wen-ping HU Yong-hong LIMing GU Ai-xia College of Life Science Agricultural University ofHebei Baoding 071001 E R. China Baoding Research Institute of Agricultural Science Baoding 071001 P. R. China College of Landscape Architecture and Tourism Agricultural University ofHebei Baoding 071001 P. R. China

somatic embryogenesis ofKoelreuteria bipinnata var. integrifoliola was observed, plantlet formation in different types of somatic embryos was studied and the effect of abnormal embryos on plantlet formation was identified. Results show that somatic embryos of K. bipinnata var. integrifoliola include normal embryos, embryos with abnormal cotyledons, vitrified embryos, albino embryos, secondary embryos, linked embryos, embryos with abnormal growing points and embryos with expanding hypocotyl. After 40 d of callus culture, the response of normal somatic embryos from K. bipinnata var. integrifoliola was 26.7%, embryos with abnormal cotyledon 30.3% while other types of somatic embryos were below the 20% level. Most of normal embryos developed into plantlets and plantlet formation reached 94.9%. But the percentage of plantlet formation decreased apparently in abnormal embryos： the number of embryos with abnormal cotyledon declined to 76.1%, that of linked embryos to 47.4% and other types of abnormal embryos to below the 20% level. Albino embryos and embryos with abnormal growing point did not develop at all into plantlets. embryos with abnormal cotyledons, linked embryos and embryos with abnormal growing points were observed during early stages of somatic embryogenesis, but vitrified, secondary and albino embryos and calli of embryos were observed at later stages. Increasing sucrose concentrations can decrease the occurrence of vitrified embryos, but the number of albino embryos decreased with an in- creasing in sucrose concentration.

关键词： Koelreuteria bipinnata var. integrifoliola somatic embryos normal embryos abnormal embryos plantlet formation

Effect of Sodium Chloride and Proline on Embryo Formation and Germination through in vitro Micropropagation of Date Palm （Phoenix dactylifera L.） cv. Barhee [11]

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Journal of Agricultural Science and Technology(B) 2013年第4期3卷 313-320页

作者： Sami A1-Zubaydi Abbas Jassim Hussain Zair Biology Department Faculty of Science University of Duhok Duhok Kurdistan Region Iraq Horticulture Department College of Agriculture University of Basrah Basrah Iraq Biology Department College of Education University of Basrah Basrah lraq

The effect of NaCI and proline on somatic embryos formation and germination of date palm （Phoenix dactylifera L.） cv. Barhee was studied. Quarters of shoot tips were cultured on MS solid growth media supplied with different concentrations of salt （NaCI）（0.5%, 1%, 1.5%, 2%, 2.5% and 3%, respectively） and proline （25, 50 and 75 mg L1） either individually or in combination. Results showed that the addition of NaC1 （starting at 1%） caused a significant increase in the cylindrical embryos formation time, while their lengths and fresh weights were decreased at salt concentrations （0.5% and 2%）. No significant effect was noticed on the formation time by using proline except at concentration of 25 mg L^-1 which caused an increase in their lengths and fresh weights. The interaction between all salt concentrations and proline （25 mg LL） caused a significant increase in the formation time as well as in their lengths and fresh weights. The addition of NaC1 to the media caused a significant effect on the germination time and a reduction on the percentages of somatic embryo germination starting at 2% and the following ones. A reduction in the lengths of radicals and the plumules of the formed embryos was noticed at 1.5% NaCI. Proline addition reduced the initial time and increased the germination percentages as well as the lengths of radicals and plumules. The interaction between salt and proline treatments caused a significant reduction in the initiation time and an increase in the germination percentages and lengths of radicals and plumules.

关键词： Phoenix dactylifera NaCI proline somatic embryos germination.

Plantlets Regeneration via somatic Embryogenesis from the Nucellus Tissues of Kinnow Mandarin (Citrus reticulata L.)

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American Journal of Plant Sciences 2016年第6期7卷 798-805页

作者： Mubashir Hussain Naveed Iqbal Raja Muhammad Iqbal Anam Iftikhar Huma Mehreen Sadaf Sidra Sabir Muhammad Asim Sultan Muhammad Nasim Ashraf Faz Department of Botany PMAS Arid Agriculture University Rawalpindi Pakistan Department of Botany University of Sargodha Sargodha Pakistan Department of Botany Government College University Faisalabad Pakistan

Studies were initiated to explore the role of nucellus tissues and growth regulators in plantlets regeneration via somatic embryogenesis of Kinnow mandarin [Citrus reticulata L. (Blanco)]. Nucellus tissues were cultured on MS medium supplemented with different concentrations and combinations of auxins, cytokinins and malt extract for primary callus induction. The best response for primary callus induction (90%) was obtained when MS medium was supplemented with 5 mg/l 2,4-D and 500 mg/l malt extract. Best results for embryogenic callus induction (80%) were obtained in C8 medium. The induction of somatic embryos was highest when MS medium was supplemented with 1 mg/l BAP and maturation of somatic embryos occurred when MS medium was supplemented with 5 mg/l 2,4-D and 1 mg/l BAP. Maximum plantlets were regenerated (92%) from the somatic embryos on half strength MS medium with no hormones. The plantlets were successfully acclimatized in different potting mixtures and highest survival rate (100%) was achieved in potting mixture containing sand and peat moss (2:1).

关键词： Tissue Culture MS Media Citrus Nucellus Tissue Callogenesis somatic embryos

somatic Embryogenesis in Lily Bulb Scale Cultures

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Journal of Northeast Agricultural University(English Edition) 2008年第4期15卷 11-14页

作者： WANG Shasha WANG Jingang FAN Jinping CHE Daidi College of Horticulture Northeast Agricultural University Harbin 150030 China

Abstract： somatic embryogenesis from lily bulb scales has not been studied in details, although tissue culture methods have been applied to the propagation for decades. The effects of different kinds and concentration of auxins for oriental lily somatic embryogenesis were investigated （Lilium hybrida var. Sorbonne）. 2, 4-dichlorophenoxyacetic acid （2, 4-D）, thidiazuron （TDZ） and α-naphthaleneacetic acid （NAA） media with benzyladenine（6-BA） and lactalbumin hydrolysate （LH） were used for embryogenic callus in the darkness. The best response on embryogenic callus formation was obtained on MS media supplemented 2, 4-D 2.0 mg·L^-1, 6-BA 0.5 mg·L^-1 and LH 300 mg·L^-1. Transfer embryogenic callus to the media with TDZ, 6-BA, kinetin （KT） supplemented 2, 4-D. The highest number of somatic embryos has been produced on medium with 0.5 mg·L^-1 2, 4-D and 0.3 mg·L^-1 KT. Germinated embryos with shoot axes were changed to MS media with 6-BA 0.5 mg·L^-1. The results suggest that in vitro culture of somatic embryogenesis from lily bulb scales can be used for plant regeneration.

关键词： lily bulb scales embryogenic callus somatic embryos in vitro

Synthetic Seed Preparation, Germination and Plantlet Regeneration of Litchi (Litchi chinensis Sonn.)

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American Journal of Plant Sciences 2016年第10期7卷 1395-1406页

作者： D. K. Das A. Rahman Dipti Kumari Nutan Kumari Post Graduate Department of Biotechnology T.M. Bhagalpur University Bhagalpur India

Litchi chinensis sonn.) ranks second after mango amongst the most important fruit crops cultivated worldwide. Litchi is a very valuable crop throughout the world because it is a table fruit and wines are also produced from it. The existing cultivars are highly polyploidy and heterozygous in nature. It is propagated through air layering and marcottage methods and storability is very low. Synthetic seeds can be stored for a long time and its genetic constitution could remain the same. For germplasm maintenance and clonal propagation, synthetic seeds can be used. somatic embryogenesis has been reported from anther or embryogenic suspension culture in various species of litchi. Regeneration via organogenesis and somatic embryogenesis from zygotic embryos has also been reported in certain species. Developing a methodology for getting somatic embryogenesis with a high frequency from zygotic embryos which is available once in a year, would be particularly useful for genetic improvement of litchi. Cotyledonary stage somatic embryos developed from zygotic embryos were encapsulated in 2% alginate gel. The encapsulated somatic embryos (ESEs) germinated successfully on 0.7% agar medium containing 3% sucrose concentration in NN basal medium (half strength of major and minor salts) with 1 mg·l-1 of gibbrellic acid. Percentage germination and plantlet development for ESEs was higher than that of non encapsulated embryos (NSEs). In comparison to different hormones, gibberellic acid has a significant influence on the germination rate of ESEs after one week of dehydration was seen maximum at 9% sucrose and abscisic acid (1 mg·l-1) in half strength of major and minor salts in Nitsch and Nitsch medium resulting in extended storage up to 90 days without loss in germination potential and capability to regenerate into plantlets. Normally developed plantlets regenerated from ESEs were successfully adapted to soil to obtain a full grown plant.

关键词： Encapsulation Germination Plantlet Regeneration somatic embryos