Morphology of somatic embryogenesis and plantlet formation in tissue cultures of lantern tree (Koelreuteria bipinnata ***)

作     者：FENG Da-ling MENG Qing-rong LI Wen-ping HU Yong-hong LIMing GU Ai-xia 

作者机构：College of Life Science Agricultural University ofHebei Baoding 071001 E R. China Baoding Research Institute of Agricultural Science Baoding 071001 P. R. China College of Landscape Architecture and Tourism Agricultural University ofHebei Baoding 071001 P. R. China 

出 版 物：《Forestry Studies in China》 (中国林学（英文版）)

年 卷 期：2010年第12卷第1期

页      面：31-36页

学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 0907[农学-林学] 07[理学] 0829[工学-林业工程] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 0713[理学-生态学] 

基　　金：supported by the Foundation for Science and Technology Research and Development Program of Hebei Province (09225511) the Project of the Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of the Ministry of Education, Beijing Forestry University, China (05– 04) 

主　　题：Koelreuteria bipinnata var. integrifoliola somatic embryos normal embryos abnormal embryos plantlet formation 

摘      要：Somatic embryogenesis ofKoelreuteria bipinnata var. integrifoliola was observed, plantlet formation in different types of somatic embryos was studied and the effect of abnormal embryos on plantlet formation was identified. Results show that somatic embryos of K. bipinnata var. integrifoliola include normal embryos, embryos with abnormal cotyledons, vitrified embryos, albino embryos, secondary embryos, linked embryos, embryos with abnormal growing points and embryos with expanding hypocotyl. After 40 d of callus culture, the response of normal somatic embryos from K. bipinnata var. integrifoliola was 26.7%, embryos with abnormal cotyledon 30.3% while other types of somatic embryos were below the 20% level. Most of normal embryos developed into plantlets and plantlet formation reached 94.9%. But the percentage of plantlet formation decreased apparently in abnormal embryos： the number of embryos with abnormal cotyledon declined to 76.1%, that of linked embryos to 47.4% and other types of abnormal embryos to below the 20% level. Albino embryos and embryos with abnormal growing point did not develop at all into plantlets. Embryos with abnormal cotyledons, linked embryos and embryos with abnormal growing points were observed during early stages of somatic embryogenesis, but vitrified, secondary and albino embryos and calli of embryos were observed at later stages. Increasing sucrose concentrations can decrease the occurrence of vitrified embryos, but the number of albino embryos decreased with an in- creasing in sucrose concentration.