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Exogenous Spermidine Promotes somatic embryogenesis of Cunninghamia lanceolata by Altering the Endogenous Phytohormone Content

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Phyton-International Journal of Experimental Botany 2020年第1期89卷 27-34页

作者： Dandan Wang Yulin Guo Xiaofei Long Yan Pan Dingjie Yang Rui Li Ye Lu Ying Chen Jisen Shi Jinhui Chen Key Laboratory of Forest Genetics&Biotechnology of Ministry of Education of China Co-Innovation Center for Sustainable Forestry in Southern ChinaNanjing Forestry UniversityNanjing210037China Jiangsu Key Laboratory for Poplar Germplasm Enhancement and Variety Improvement Nanjing Forestry University Nanjing210037China

In order to study how exogenous hormones in ***(gymnosperm)regulate somatic embryogenesis,we measured the endogenous phytohormones of two genotypes with different somatic embryogenesis efficiency and found that an increase in endogenous concentrations of IAA and ABA may be correlated to more efficient somatic *** applying exogenous spermidine,we found that exogenous hormones may affect somatic embryogenesis efficiency through affecting the endogenous phytohormone *** on these results,further studies can be conducted whereby the concentration of exogenous hormones or the levels of endogenous phytohormones by molecular methods are regulated to promote somatic *** research may benefit the long-term economic output of the forestry industry and lays the foundation to studying the molecular mechanism that controls somatic embryogenesis efficiency.

关键词： Spermidine C.lanceolata somatic embryogenesis endogenous phytohormones

Comparative transcriptome study provides insights into acquisition of embryogenic ability in upland cotton during somatic embryogenesis

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Journal of Cotton Research 2018年第2期1卷 44-56页

作者： SUN Ruibin TIAN Ruiping MA Dan WANG Shaohui LIU Chuanliang State Key Laboratory of Cotton Biology Institute of Cotton ResearchChinese Academy of Agriculture Sciences Zhengzhou Research Base State Key Laboratory of Cotton BiologyZhengzhou University

Background： The conversion from non-embryogenic callus （NEC） to embryogenic callus （EC） is the key bottleneck step in regeneration of upland cotton （Gossypium hirsutum）, and hinders the transgenic breeding of upland cotton. To investigate molecular mechanisms underlying acquisition of embryogenic potential during this process, comparation analysis of transcriptome dynamics between two upland cotton cultivars with different somatic embryogenesis abilities was conducted. Results： Differentially expressed genes involved in the transformation from NEC to EC were detected in the two different cultivars. Principal component analysis based on DEGs showed that the NEC tissues of the two cultivars were highly heterogeneous, whereas the derived EC tissues were similar, which suggested the homogeneousness of EC between different lines. In the highly embryogenic cultivar CCRI 24, more of these genes were down-regulated, whereas, in the recalcitrant cultivar CCRI 12, more were up-regulated. Bioinformatics analysis on these DEGs showed that the vast majority of differentially expressed genes were enriched in metabolism and secondary metabolites biosynthesis pathways. Flavonoid biosynthesis and phenylpropanoid biosynthesis pathways were enriched in both cultivars, and the associated genes were down-regulated more in CCRI 24 than in CCRI 12. We deduced that vigorous secondary metabolism in CCRI 12 may hinder primary metabolism, resulting in tardiness of cell differentiation. Interestingly, genes involved in the plant hormone signal transduction pathway were enriched in the recalcitrant cultivar CCRI 12, but not in CCRI 24, suggesting more radical regulation of hormone signal transduction in the recalcitrant cultivar. Signal transduction rather than biosynthesis of plant hormones is more likely to be the determining factor triggering NEC to EC transition in recalcitrant cotton lines. Transcription factor encoding genes showed differential regulation between two cultivars. Conclusion

关键词： Upland cotton Transcriptome Non-embryogenic callus (NEC) Embryogenic callus (EC) somatic embryogenesis

Overexpressing rice lesion simulating disease 1-like gene(OsLOL1)in Gossypium hirsutum promotes somatic embryogenesis and plant regeneration

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Journal of Cotton Research 2020年第3期3卷 149-155页

作者： WANG Zhian WANG Peng HU Guang XIAO Juanli ZHANG Anhong LUO Xiaoli WU Jiahe Institute of Cotton Research/Shanxi Academy of Agricultural Sciences/Shanxi Agricultural University Yuncheng 044000China State Key Laboratory of Cotton Biology/Institute of Cotton Research Chinese Academy of Agricultural SciencesAnyang 455000China State Key Laboratory of Plant Genomics/Institute of Microbiology Chinese Academy of SciencesBeijing 100101China

Background:Cotton somatic embryogenesis is difficult or rarely frequent to present,which has limited gene function identification and biotechnological ***,we employed a rice key somatic embryogenesis-related gene,rice lesion simulating disease 1-like gene(OsLOL1),to develop transgenic cotton callus for evaluating its function in ectopic ***:Overexpressing OsLOL1 can promote cotton callus to form embryogenic callus,not only shortening time but also increasing transition of somatic callus cells to embryogenic callus *** the regenerating plantlets per transgenic OsLOL1 embryogenic callus were significantly higher than those in the control transformed with empty *** of physiological and biochemical showed that OsLOL1 can repress cotton superoxide dismutase 1 gene(GhSOD1)expression,possibly resulting in reactive oxidant species(ROS)accumulation in transgenic callus *** OsLOL1-overexpressed embryogenic callus exhibited higherα-amylase activity compared with the control,resulting from the promotion of OsLOL1 to cotton amylase 7 gene(GhAmy7)and GhAmy8 ***:The data showed that OsLOL1 could be used as a candidate gene to transform cotton to increase its somatic embryogenesis capacity,facilitating gene function analysis and molecular breeding in cotton.

关键词： Gossypium hirsutum somatic embryogenesis OsLOL1 GhSOD1 GhAmy7

somatic embryogenesis from Cell Suspension Cultures of Aspen Clone

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Forestry Studies in China 2005年第3期7卷 43-46页

作者： Yu Zhi-shui Zhang Zhi-yi Luo Jun-min Hu Chong-fu He Cheng-zhong Wang Ze-liang Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants Ministry of Education Beijing Forestry University Beijing 100083 P. R. China

Suspension cultures initiated from callus derived from petiole explants of aspen hybrid （Populus tremuloides×P. tremula） produced somatic embryos. Callus was induced on a MS medium supplemented with 5mg·L^-1 2,4-D and 0.05mg·L^-1 zeatin under light conditions. Embryogenic calli were obtained when a subsequent subculture of calli was suspended in the same basal medium with 10mg·L^-1 2,4-D. The highest number of globular embryos were induced from embryogenic calli by cell suspension culture in a MS liquid medium supplemented with 10mg·L^-1 2,4-D. Genotype and 2,4-D concentration were vital to the induction of embryogenic calli producing competent cells. Embryogenic calli for each genotype were heterogeneous. Green calli with gel-like consistency could yield more competent cells than light yellow embryogenic calli. However, some globular embryos broke into slices and some developed abnormally after one month of culture under the same or other hormonal conditions.

关键词： Populus callus cell suspension somatic embryogenesis

Effect of plant growth regulators on direct somatic embryogenesis in camphor tree(Cinnamomum camphora L.)from immature zygotic embryos and embryogenic calli induction

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Forestry Studies in China 2007年第4期9卷 267-271页

作者： Du Li Zhou Suo Bao Man-zhu Shool of Life Science and Technology Nanyang Normal University Nanyang 473000 P. R. China Key Laboratory of Horticultural Plant Biology of the Ministry of Education College of Horticulture and Forestry Sciences Huazhong Agricultural University Wuhan 430070 P. R. China

A description of a successful direct somatic embryogenesis induction from immature zygotic embryos of a camphor tree （Cinnamomum camphora L.） is presented. After a subculture of 2-3 years, embryogenic calli could be derived from primary somatic embryos. Immature zygotic embryos were cultured on a Murashige and Skoog （MS） basal medium supplemented with a range of combinations of cytokinins （BA） and auxins （2,4-D or NAA） for somatic embryo induction. Primary somatic embryos could be induced directly in almost all PGR combinations. A positive effect of 2,4-D on somatic embryogenesis from immature zygotic embryos of camphor tree was obtained. BA at appropriate concentrations （〈 5 mg-L-1） had an effect similar to 2,4-D, whereas high concentrations （〉 5 mg·L^-1） of BA had the effect of restraining somatic embryo induction. NAA had a less positive effect on somatic embryogenesis than 2,4-D.

关键词： Cinnamomum camphora L. somatic embryogenesis primary somatic embryo secondary somatic embryogenesis em-bryogenic calli

Studies on the Factors Affecting on somatic embryogenesis in Soybean

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Agricultural Sciences in China 2002年第9期1卷 960-964页

作者： WANG Ping, WANG Gang, LU Wen-he, JI Jing and YANG Qing-kai(Laboratory of Plant Molecular Biology, The Research Center of Plant Genetic Engineering, The Quartermaster University of PLA , Changchun 130062 , P.R. China Agricultural College , Northeast Agricultural University, Harbin 150030 , P.R. China) Laboratory of Plant Molecular Biology The Research Center of Plant Genetic Engineering The Quartermaster University of PLA Changchun 130062 P.R.China Agricultural College Northeast Agricultural University Harbin 150030 P.R.China Laboratory of Plant Molecular Biology The Research Center of Plant Genetic Engineering The Quartermaster University of PLA Changchun 130062 P. R . China Agricultural College Northeast Agricultural University Harbin 150030 P. R . China

The effects of the concentration of MS micro salts, 2,4-dichlorophenoxyacetic acid (2,4-D), 3-naphthalene acetic acid (NAA), proline and adenine on callus formation and somatic embryogenesis were investigated using orthogonal design with immature cotyledons of soybean. The results showed that the role of concentration of micro salts on frequency of callus formation and somatic embryogenesis were significant. MS medium supplemented with 2,4-D, NAA, proline and adenine could stimulate callus formation. The concentration of MS micro salts possessed obvious effects on somatic embryogenesis of *** category and concentration of the hormone needed were different among genotypes when embryogenesis were induced.

关键词： Soybean (Glycine max) somatic embryogenesis Genotype Medium

somatic embryogenesis and peroxidase activity of desiccation tol-erant mature somatic embryos of loblolly pine

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Journal of Forestry Research 2001年第3期12卷 147-152,209页

作者：唐巍 North Carolina State University

White, translucent, glossy mucilaginous callus was initiated from the mature zygotic embryos explants on callus induction medium with 2,4-D, BA, and kinetin in the 3-9th week of culture. This type of callus induction occurred at a lower fre-quency with either a-naphthaleneacetic acid (NAA) or IBA (both 8 mg/L). White, translucent, glossy mucilaginous callus was embryogenic and mainly developed from the cotyledons of the mature zygotic embryo. somatic embryos were formed on dif-ferentiation medium. Desiccation tolerance can be induced by culturing somatic embryos of loblolly pine (Pinus taeda L.) on medium supplemented with 50 mm abscisic acid (ABA) and/or 8.5% polyethylene glycol (PEG6000). Scanning electron micros-copy of desiccated somatic embryos showed that the size and external morphology of the desiccation tolerant somatic embryos recovered to the pre-desiccation state within 24-36 h, whereas the sensitive somatic embryos did not recover and remained shriveled, after the desiccated somatic embryos had been rehydrated. Peroxidase activity of desiccated somatic embryos in-creased sharply after 3 days of desiccation treatment, and desiccation tolerant somatic embryos had higher peroxidase activity compared to sensitive somatic embryos. Higher peroxidase activity of desiccation tolerant somatic embryos was possibly ad-vantage of catalyzing the reduction of H2O2 which was produced by drought stress, and protecting somatic embryos from oxida-tive damage.

关键词： Pinus taeda L. somatic embryogenesis Desiccation tolerance Peroxidase activity

somatic embryogenesis and Plant Regeneration from Leaflets of Fritillaria ussuriensis M.

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Journal of Northeast Agricultural University(English Edition) 1995年第2期2卷 11-11页

作者： Tang Wei (Northeast Agricultural University, Harbin 150030, P R C)

A method for the production of somatic embryos and subsequent plant regeneration for Fritillaria ussuriensis M. is described. Whole leaflet cxplants, derived from plantlets grown in vitro, formed light yellowish embryogenic calli within one month of culture in the dark. somatic embryogenesis was obtained after a 28 d incubation on MS induction medium supplemented with 2 mg / L 2,4-D 0.5 mg / L BA, 0.5 mg / L KT and 500 mg / L CH followed by transfer to a second N medium containing 0.5 mg / L KT and 100 mg / L CH. somatic embryos were transferred to MS medium with 0.1mg/ L NAA and placed in the light for regeneration. After two weeks, mature somatic embryo developed into whole plantlet.

关键词： Fritillaria ussuriensis M. somatic embryogenesis plant regencration

Stress Treatments and DNA Methylation Affected the somatic embryogenesis of Citrus Callus

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Acta Botanica Sinica 2002年第6期44卷 673-677页

作者：郝玉金邓秀新华中农业大学作物遗传改良国家重点实验室武汉430070

The evaluation on the callus embryogenesis capacity of 15 genotypes of citrus showed that stress treatments were conducive to somatic embryogenesis and could enhance the recovery of the missed capacity of embryogenesis for some genotypes. Randomly amplified polymorphic DNA (RAPD) and methylation sensitive amplified polymorphism (MSAP) analysis indicated that there existed significant differences in DNA methylation status between the callus capable of producing somatic embryoids and that which missed the embryogenesis capacity of the same genotype Newhall navel orange ( Citrus sinensis Osb. cv. Newhall). The DNA methylation level of the former was lower than that of the latter. However, RAPD profiles did not show any difference between these two kinds of callus.

关键词： desiccation low_temperature treatment DNA methylation somatic embryogenesis RAPD MSAP