Somatic Embryogenesis and Plant Regeneration from Leaflets of Fritillaria ussuriensis M.

作     者：Tang Wei (Northeast Agricultural University, Harbin 150030, P R C) 

出 版 物：《Journal of Northeast Agricultural University(English Edition)》 (东北农业大学学报（英文版）)

年 卷 期：1995年第2卷第2期

页      面：11-11页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 0828[工学-农业工程] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

主　　题：Fritillaria ussuriensis M. somatic embryogenesis plant regencration 

摘      要：A method for the production of somatic embryos and subsequent plant regeneration for Fritillaria ussuriensis M. is described. Whole leaflet cxplants, derived from plantlets grown in vitro, formed light yellowish embryogenic calli within one month of culture in the dark. Somatic embryogenesis was obtained after a 28 d incubation on MS induction medium supplemented with 2 mg / L 2,4-D 0.5 mg / L BA, 0.5 mg / L KT and 500 mg / L CH followed by transfer to a second N medium containing 0.5 mg / L KT and 100 mg / L CH. Somatic embryos were transferred to MS medium with 0.1mg/ L NAA and placed in the light for regeneration. After two weeks, mature somatic embryo developed into whole plantlet.