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small intestinal submucosa improves islet survival and function during in vitro culture

World Journal of Gastroenterology 2005年第46期11卷 7378-7383页

作者： Xiao-Hui Tian Wu-Jun Xue Xiao-Ming Ding Xin-Lu Pang Yan Teng Pu-Xun Tian Xin-Shun Feng Center of Renal Transplantation The First Hospital of Xi'an Jiaotong UniversityXi'an 710061 Shaanxi Province China

AIM： To evaluate the recovery and function of isolated rat pancreatic islets during in vitro culture with small intestinal submucosa （SIS）. METHODS： Pancreatic islets were isolated from Wistar rats by standard surgical procurement followed by intraductal collagenase distension, mechanical dissociation and Euroficoll purification. Purified islets were cultured in plates coated with multilayer SIS （SIS-treated group） or without multilayer SIS （standard cultured group） for 7 and 14 d in standard islet culture media of RPMI 1640. After isolation and culture, islets from both experimental groups were stained with dithizone and counted. Recovery of islets was determined by the ratio of counts after the culture to the yield of islets immediately following islet isolation. Viability of islets after the culture was assessed by the glucose challenge best with low （2.7 mmol/L） and high glucose （16.7 mmol/L） solution supplemented with 50 mmol/L 3-isobutyl-1- methylxanthine （IBMX） solution. Apoptosis of islet cells after the culture was measured by relative quantification of histone-complexed DNA fragments using ELISA. RESULTS： After 7 or 14 d of in vitro tissue culture, the recovery of islets in SIS-treated group was significantly higher than that cultured in plates without SIS coating. The recovery of islets in SIS-treated group was about twice more than that of in the control group. In SIS treated group, there was no significant difference in the recovery of islets between short- and long-term periods of culture （95.8±1.0% vs 90.8±1.5%, P〉0.05）. When incubated with high glucose （16.7 mmol/L） solution, insulin secretion in SIS-treated group showed a higher increase than that in control group after 14 d of culture （20.7±1.1 mU/L vs 11.8±1.1 mU/L, P〈0.05）. When islets were placed in high glucose solution containing IBMX, stimulated insulin secretion was higher in SlS-treated group than in control group. Calculated stimulation index of SlS-treated group was about 23 times of

关键词： Islet culture Islet survival Islet function small intestinal submucosa

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Tissue engineered esophagus by copper——small intestinal submucosa graft for esophageal repair in a canine model

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Science China(Life Sciences) 2014年第2期57卷 248-255页

作者： TAN Bo WANG Min CHEN Xi HOU JiangLong CHEN XiaoHe WANG Yue LI-LING Jesse XIE HuiQi Regenerative Medicine Research Center West China Hospital Sichuan University Laboratory of Stem Cell and Tissue Engineering State Key Laboratory of Biotherapy West China Hospital Sichuan University Department of Orthopedics Sichuan Academy of Medical Sciences Sichuan Provincial People's Hospital Department of Thoracic and Cardiovascular Surgery West China Hospital Sichuan University Institute of Genetic Medicine School of Life Sciences Sichuan University

Acellular porcine small intestinal submucosa(SIS)has been used for esophagoplasty with success in a canine ***,it did not lead to complete *** better reconstruction,a cellular component is ***,promotion of angiogenesis with copper has been widely recognized by basic research as well as clinical *** this study,we have evaluated the feasibility and effectiveness of combined Cu and SIS(SIS-Cu patch)for the esophageal repair using a canine *** male beagle dogs were subjected to surgical resection to produce cervical esophageal defects(5 cm in length,180°in range).SIS with Cu(5 or 25μmol L 1copper)or without Cu was patched on the esophageal *** esophagram and histology exam were carried out to evaluate the effectiveness of the *** shown,the SIS-Cu graft promoted re-epithelialization,re-vascularization and muscular ***-Cu patch is more effective than SIS alone for esophageal repair,and the SIS+25μmol L 1Cu group demonstrated additional advantages over the SIS+5μmol L 1Cu.

关键词： esophagus re-epithelialization re-vascularization muscle regeneration copper small intestinal submucosa

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Co-administration of platelet-rich plasma and small intestinal submucosa is more beneficial than their individual use in promoting acute skin wound healing

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Burns & Trauma 2021年第1期9卷 72-82页

作者： Xiaoxuan Lei Liuhanghang Cheng Yu Yang Mengru Pang Yunqing Dong Xuanru Zhu Caihong Chen Zexin Yao Gang Wu Biao Cheng Tymour Forouzanfar Department of Oral and Maxillofacial Surgery/Pathology Amsterdam UMC and Academic Center for Dentistry Amsterdam(ACTA)Vrije Universiteit AmsterdamAmsterdam Movement ScienceAmsterdam1081HVThe Netherlands Department of Burn and Plastic Surgery General Hospital of Southern Theater CommandGuangzhou510030China Department of Oral Implantology and Prosthetic Dentistry Academic Center for Dentistry Amsterdam(ACTA)University of Amsterdam(UvA)and Vrije Universiteit Amsterdam(VU)Amsterdam1081LAThe Netherlands

Background:Acute skin wounds may compromise the skin barrier,posing a risk of *** intestinal submucosa(SIS)is widely used to treat acute and chronic ***,the efficacy of SIS to accelerate wound healing still needs to be improved to meet clinical *** tackle this problem,platelet-rich plasma(PRP)is used due to its potency to promote proliferation,migration and adhesion of target *** this study,we applied PRP and SIS to skin wounds to explore their effects on wound healing by evaluating re-epithelialization,collagen production,angiogenesis and the inflammatory ***:A1×1-cm full-thickness skin defectwas established in *** mice were divided into four treatment groups:PRP+SIS,PRP,SIS and *** days 3,5,7,10 and 14 post-surgery,tissue specimens were *** and eosin,Masson’s trichrome,immunohistochemical and immunofluorescence double staining were used to visualize epidermal thickness,collagen and vascular regeneration and ***:Wound contraction in the PRP and PRP+SIS groups was significantly greater,compared with the other groups,on days 3 and 5 post-surgery.A histological analysis showed higher collagen expression in the PRP and PRP+SIS groups on day 7,whichwas associated with a thicker epidermal layer on day *** addition,immunohistochemical staining showed that CD31-positive blood vessels and vascular endothelial growth factor expression in the PRP+SIS and PRP groups were significantly higher,compared with the control ***,immunofluorescence double staining showed that the number of M1 and M2 macrophages in the PRP+SIS and PRP groups was higher,compared with the control and SIS groups alone,on day ***,on day 7,the number of M1 macrophages dramatically decreased in the PRP+SIS and PRP *** ratio of M2 to M1 macrophages in the PRP+SIS and PRP groups was 3.97 and 2.93 times that of the control group and 4.56 and 3.37 times that of the SIS group,respectively.

关键词： small intestinal submucosa Platelet-rich plasma Full-thickness skin defect M1macrophages M2macrophages Animal model wound healing skin

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Porcine small intestinal submucosa Mesh for Treatment of Pelvic Organ Prolapsed

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Chinese Medical Journal 2016年第21期129卷 2603-2609页

作者： Ting-Ting Cao Xiu-Li Sun Shi-Yan Wang Xin Yang Jian-Liu Wang Department of Gynecology Peking University People's Hospital Beijing 100044 China

Background：Pelvic organ prolapse （POP） is a major health concern that affects *** have increasingly used prosthetic meshes to correct ***,the most common used is synthetic mesh,and absorbable mesh is less *** research aimed to evaluate the clinical effectiveness of porcine small intestinal submucosa （SIS）.Methods：Consecutive forty POP patients who met the inclusion criteria underwent pelvic reconstruction surgery with SIS between March 2012 and December *** patients＆#39; clinical characteristics were recorded *** outcomes,measured by objective and subjective success rates,were *** evaluated the quality of life （QOL） using the Pelvic Floor Distress Inventory-20 （PFDI-20） and the Pelvic Floor Impact Questionnaire-7 （PFIQ-7）.Sexual QOL was assessed by the Pelvic Organ Prolapse/Urinary Incontinence Sexual Function Questionnaire-12 （P1SQ-12）.Results：At postoperative 12 months,the subjective recurrence rate （7.5%） was much lower than the objective recurrence rate （40.0%）.Postoperatively,no erosion was *** underwent a graft release procedure because of urinary retention,and one had anus sphincter reconstruction surgery due to defecation *** experienced posterior vaginal wall infection where the mesh was implanted,accompanied by severe vaginal *** cream relieved the *** patient with recurrence underwent a secondary surgery with Bard Mesh because of stage 3 anterior vaginal wall *** system of PFDI-20 was from 59.150 ± 13.143 preoperatively to 8.400 ± 4.749 postoperatively and PFIQ-7 was from 73.350 ± 32.281 to 7.150 ± 3.110,while PISQ-12 was from 15.825 ± 4.050 to 12.725 ± ***：QOL and the degree of subjective satisfaction were significantly improved *** repair deserves more attention because of the higher recurrence *** long-term follow-up of the patient is warranted to draw firm conclusion.

关键词： Lower Urinary Tract Symptoms, Pelvic Organ Prolapse Quality of Life Recurrence small intestinal submucosa

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Surface modification of small intestine submucosa in tissue engineering

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Regenerative Biomaterials 2020年第4期7卷 339-348页

作者： Pan Zhao Xiang Li Qin Fang Fanglin Wang Qiang Ao Xiaohong Wang Xiaohong Tian Hao Tong Shuling Bai Jun Fan Department of Tissue Engineering School of Fundamental SciencesChina Medical University77 Puhe AvenueShenbei New DistrictShenyang 110122China Department of Cell Biology School of Life SciencesChina Medical University77 Puhe AvenueShenbei New DistrictShenyang 110122China and 3Cardiac Surgery Liaoning First Hospital of China Medical UniversityNo.155 Nanjing StreetHeping DistrictShenyangLiaoning 110122China

With the development of tissue engineering,the required biomaterials need to have the ability to promote cell adhesion and proliferation in vitro and in ***,surface modification of the scaffold material has a great influence on biocompatibility and functionality of *** small intestine submucosa(SIS)is an extracellular matrix isolated from the submucosal layer of porcine jejunum,which has good tissue mechanical properties and regenerative activity,and is suitable for cell adhesion,proliferation and *** recent years,SIS is widely used in different areas of tissue reconstruction,such as blood vessels,bone,cartilage,bladder and ureter,*** paper discusses the main methods for surface modification of SIS to improve and optimize the performance of SIS bioscaffolds,including functional group bonding,protein adsorption,mineral coating,topography and formatting modification and drug *** addition,the reasonable combination of these methods also offers great improvement on SIS surface *** article makes a shallow review of the surface modification of SIS and its application in tissue engineering.

关键词： small intestinal submucosa surface modification in vitro scaffold tissue engineering

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Procyanidins-crosslinked small intestine submucosa: A bladder patch promotes smooth muscle regeneration and bladder function restoration in a rabbit model

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Bioactive Materials 2021年第6期6卷 1827-1838页

作者： Xiu-Zhen Zhang Yan-Lin Jiang Jun-Gen Hu Long-Mei Zhao Qiu-Zhu Chen Yan Liang Yi Zhang Xiong-Xin Lei Rui Wang Yi Lei Qing-Yi Zhang Jesse Li-Ling Hui-Qi Xie Laboratory of Stem Cell and Tissue Engineering Orthopedic Research InstituteState Key Laboratory of Biotherapy and Cancer CenterWest China HospitalSichuan University and Collaborative Innovation Center of BiotherapyChengduSichuanChina Research Core Facility of West China Hospital Sichuan UniversityChengduSichuanChina

Currently the standard surgical treatment for bladder defects is augmentation cystoplasty with autologous tissues,which has many side *** such as small intestine submucosa(SIS)can provide an alternative scaffold for the repair as bladder *** studies have shown that SIS could enhance the capacity and compliance of the bladder,but its application is hindered by issues like limited smooth muscle regeneration and stone formation since the fast degradation and poor mechanical properties of the ***(PC),a natural bio-crosslinking agent,has shown anti-calcification,anti-inflammatory and anti-oxidation *** importantly,PC and SIS can crosslink through hydrogen bonds,which may endow the material with enhanced mechanical property and stabilized *** this study,various concentrations of PC-crosslinked SIS(PC-SIS)were prepared to repair the full-thickness bladder defects,with an aim to reduce complications and enhance bladder *** vitro assays showed that the crosslinking has conferred the biomaterial with superior mechanical property and anti-calcification property,ability to promote smooth muscle cell adhesion and upregulate functional genes *** a rabbit model with bladder defects,we demonstrated that the PC-SIS scaffold can rapidly promote in situ tissue regrowth and regeneration,in particular smooth muscle remodeling and improvement of urinary *** PC-SIS scaffold has therefore provided a promising material for the reconstruction of a functional bladder.

关键词： Bladder reconstruction small intestinal submucosa Procyanidins Bio-crosslinking Smooth muscle regeneration

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Enhancing cartilage regeneration and repair through bioactive and biomechanical modification of 3D acellular dermal matrix

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Regenerative Biomaterials 2024年第3期3卷 105-121页

作者： Wei Gao Tan Cheng Zhengya Tang Wenqiang Zhang Yong Xui Min Han Guangdong Zhou Chunsheng Tao Ning Xu Huitang Xia Weijie Sun Qingdao Medical College of Qingdao University Qingdao266071China Department of Infectious Diseases The First Affliated Hospital of Anhui Medical UniversityShushanHefei230022China Department of Orthopaedic Surgery Shanghai Sixth People's Hospital Affliated to Shanghai jiao Tong University School of MedicineShanghai200025China Department of Orthopedic Surgery Shanghai Eighth People's HospitalShanghai200235China Department of PlasticSurgery&Jinan ClinicalResearch Center for Tissue Engineering Skin Regeneration and Wound Repair The First Affliated Hospital of Shandong First Medical UniversityJinan266299China Department of Orthopaedics Ninety-seventh Hospital of the Chinese People's Liberation Army NavyQingdao266071China Department of Orthopaedics The First Affliated Hospital of Shandong First Medical UniversityJinan266299China Department of Plastic surgery Shanghai Key Laboratory of Tissue EngineeringShanghai Ninth People's HospitalShanghai jiao Tong University School of MedicineShanghai200023China Department of Thoracic Surgery Shanghai Children's HospitalShanghai Jiao Tong University School of MedicineShaghai200040China Department of Thoracic Surgery Shanghai Pulmonary HospitalSchool of MedicineTongji UniversityShanghai200433China

Acellular dermal matrix(ADM)shows promise for cartilage regeneration and ***,an effective decellularization technique that removes cellular components while preserving the extracellular matrix,the transformation of 2D-ADM into a suitable 3D scaffold with porosity and the enhancement of bioactive and biomechanical properties in the 3D-ADM scaffold are yet to be fully *** this study,we present an innovative decellularization method involving 0.125%trypsin and 0.5%SDS and a 1%Triton X-100 solution for preparing ADM and converting 2D-ADM into 3D-ADM *** scaffolds exhibit favorable physicochemical properties,exceptional biocompatibility and significant potential for driving cartilage regeneration in vitro and in *** further enhance the cartilage regeneration potential of 3D-ADM *** incorporated porcine-derived small intestinal submucosa(SIS)for bioactivity and calcium sulfate hemihydrate(CSH)for biomechanical *** resulting 3D-ADM+SIS scaffolds displayed heightened biological activity,while the 3D-ADM+CSH scaffolds notably bolstered biomechanical *** scaffold types showed promise for cartilage regeneration and repair in vitro and in vivo,with considerable improvements observed in repairing cartilage defects within a rabbit articular cartilage *** summary,this research introduces a versatile 3D-ADM scaffold with customizable bioactive and biomechanical properties,poised to revolutionize the field of cartilageregeneration.

关键词： acellular dermal matrix three-dimensional scaffolds cartilage regeneration small intestinal submucosa calcium sulfate hemihydrate

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Analysis of mechanical response of fistula plug for structure optimization

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Theoretical & Applied Mechanics Letters 2012年第1期2卷 36-39页

作者： Shunli Yang Bin Jiang Feng Xu Min Lin Guiping Zhao Tianjian Lu Biomedical Engineering and Biomechanics Center Xi'an Jiaotong UniversityXi'an 710049China NanJing Hospital of T.C.M Nanjing 210001China The Key Laboratory of Biomedical Information Engineering of Ministry of Education School of Life Science and TechnologyXi'an Jiaotong UniversityXi'an 710049China

Anal fistula is one of the three greatest anorectal diseases with a high prevalence. The traditional treatments（e.g., surgery） for fistula have limitations due to damage to the internal anal sphincter of patients. With recent advances in biomaterials, treatments based on biomaterial filling （e.g., scleraprotein injection, fistula plug） have emerged as novel therapies for fistula. The anal fistula plug （e.g., based on small intestinal submucosa （SIS）） has attracted increasing attention because of short term healing rate and biocompatibility. However, challenges remain for this method such as plug falling as observed in clinics. To address this, this paper analyzes the case of SIS falling under physiological condition from mechanical point of view using ANSYS simulation. It then proposes three new geometrical structures for fistula plug and compares their mechanical behavior （e.g., axial stress, reaction of constraint） with that of clinically used structure （cone shape）. Based on the simulation, it optimizes the geometric parameters of fistula plug. The approach developed here can help to improve the design of fistula plug for better clinical treatments.

关键词： anal fistula small intestinal submucosa fistula plug structure optimization numerical simulation

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