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Protective effect of hydrogen-rich saline on ischemia/reperfusion injury in rat skin flap

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第5期14卷 382-391页

作者： Ling ZHAO You-bin WANG Shi-rui QIN Xue-mei MA Xue-jun SUN Ming-lian WANG Ru-gang ZHONG College of Life Science and Bioengineering Beijing University of Technology Peking Union Medical College Hospital Department of Diving Medicine Faculty of Naval Medicinethe Second Military Medical University

Objective: skin damage induced by ischemia/reperfusion (I/R) is a multifactorial process that often occurs in plastic surgery. The mechanisms of I/R injury include hypoxia, inflammation, and oxidative damage. Hydrogen gas has been reported to alleviate cerebral I/R injury by acting as a free radical scavenger. Here, we assessed the protective effect of hydrogen-rich saline (HRS) on skin flap I/R injury. Methods: Abdominal skin flaps of rats were elevated and ischemia was induced for 3 h; subsequently, HRS or physiological saline was administered intraperitoneally 10 min before reperfusion. On postoperative Day 5, flap survival, blood perfusion, the accumulation of reactive oxygen species (ROS), and levels of cytokines were evaluated. Histological examinations were performed to assess inflammatory cell infiltration. Results: skin flap survival and blood flow perfusion were improved by HRS relative to the controls. The production of malondialdehyde (MDA), an indicator of lipid peroxidation, was markedly reduced. A multiplex cytokine assay revealed that HRS reduced the elevation in the levels of inflammatory cytokines, chemokines and growth factors, with the exception of RANTES (regulated on activation, normal T-cell expressed and secreted) growth factor. HRS treatment also reduced inflammatory cell infiltration induced by I/R injury. Conclusions: Our findings suggest that HRS mitigates I/R injury by decreasing inflammation and, therefore, has the potential for application as a therapy for improving skin flap survival.

关键词： Hydrogen-rich saline Ischemia/reperfusion skin flaps Oxidative damage Inflammation

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Synergistic effect of hyperbaric oxygen preconditioning and hydrogen-rich saline in ameliorating rat flap ischemia/reperfusion injury

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Plastic and Aesthetic Research 2015年第1期2卷 332-339页

作者： Yi-Ding Xiao Yun-Qi Liu Ming-Zi Zhang You-Bin Wang Yi-Fang Liu Xue-Mei Ma Department of Plastic Surgery Peking Union Medical College HospitalDongcheng 100730BeijingChina College of Life Science and Bioengineering Beijing University of TechnologyChaoyang 100124BeijingChina

Aim:This study was conducted to evaluate the synergistic effects of hyperbaric oxygen(HBO)preconditioning and hydrogen-rich saline(HRS)treatment on skin flap survival and apoptosis in a rat ischemia/reperfusion(IR)skin flap ***:Male Sprague-Dawley rats were randomly divided into five groups:one sham surgery group(sham group)and four surgery groups(IR group,HBO group,HRS group,and HBO+HRS group).An extended epigastric adipocutaneous flap(6 cm×9 cm)was raised over the abdomen in each animal of all five *** last four groups underwent 6 h of IR management and were treated,respectively,with normal saline,HBO,HRS(HRS,0.8 mmol/L),or a combined approach(HBO and HRS).On the 3rd postoperative day,flap survival rate and perfusion condition,apoptotic index,caspase-3 activity,protein expression of pASK1 and Bcl-2/Bax ratio,and Bcl-2 messenger RNA(mRNA)expression were ***:Prior studies have shown the protective effects of HBO and HRS,both of which have been associated with an increase in flap *** to the IR group,the flaps in the HBO,HRS,and HBO+HRS groups showed better perfusion and a larger survival area with a low number of apoptotic cells,low caspase-3 activity and pASK1 expression,and a high Bcl-2/Bax ratio and Bcl-2 mRNA *** these groups,the HBO+HRS group showed the best flap ***:Both HBO and HRS treatments increase the rate of flap survival,while the synergistic application of HBO and HRS showed a higher survival rate as compared to individual treatments of *** potential regulation of apoptosis with the use of these two modalities may improve skin flap survival.

关键词： Apoptosis hydrogen‑rich saline hyperbaric oxygen ischemia/reperfusion injury skin flaps

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