Synergistic effect of hyperbaric oxygen preconditioning and hydrogen-rich saline in ameliorating rat flap ischemia/reperfusion injury

作     者：Yi-Ding Xiao Yun-Qi Liu Ming-Zi Zhang You-Bin Wang Yi-Fang Liu Xue-Mei Ma 

作者机构：Department of Plastic SurgeryPeking Union Medical College HospitalDongcheng 100730BeijingChina College of Life Science and BioengineeringBeijing University of TechnologyChaoyang 100124BeijingChina 

出 版 物：《Plastic and Aesthetic Research》 (整形与美容研究（英文版）)

年 卷 期：2015年第2卷第1期

页      面：332-339页

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：Project supported by the National Natural Science Foundation of China(No.81171874) the Beijing Natural Science Foundation(No.7132169) 

主　　题：Apoptosis hydrogen‑rich saline hyperbaric oxygen ischemia/reperfusion injury skin flaps 

摘      要：Aim:This study was conducted to evaluate the synergistic effects of hyperbaric oxygen(HBO)preconditioning and hydrogen-rich saline(HRS)treatment on skin flap survival and apoptosis in a rat ischemia/reperfusion(IR)skin flap ***:Male Sprague-Dawley rats were randomly divided into five groups:one sham surgery group(sham group)and four surgery groups(IR group,HBO group,HRS group,and HBO+HRS group).An extended epigastric adipocutaneous flap(6 cm×9 cm)was raised over the abdomen in each animal of all five *** last four groups underwent 6 h of IR management and were treated,respectively,with normal saline,HBO,HRS(HRS,0.8 mmol/L),or a combined approach(HBO and HRS).On the 3rd postoperative day,flap survival rate and perfusion condition,apoptotic index,caspase-3 activity,protein expression of pASK1 and Bcl-2/Bax ratio,and Bcl-2 messenger RNA(mRNA)expression were ***:Prior studies have shown the protective effects of HBO and HRS,both of which have been associated with an increase in flap *** to the IR group,the flaps in the HBO,HRS,and HBO+HRS groups showed better perfusion and a larger survival area with a low number of apoptotic cells,low caspase-3 activity and pASK1 expression,and a high Bcl-2/Bax ratio and Bcl-2 mRNA *** these groups,the HBO+HRS group showed the best flap ***:Both HBO and HRS treatments increase the rate of flap survival,while the synergistic application of HBO and HRS showed a higher survival rate as compared to individual treatments of *** potential regulation of apoptosis with the use of these two modalities may improve skin flap survival.