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Bio-Design and Manufacturing 2022年第2期5卷 249-264页

作者： Xiaocheng Wang Ruibo Zhao Jian Wang Xinghuan Li Lijuan Jin Wenyu Liu Lifang Yang Yonghua Zhu Zhikai Tan College of Biology Hunan UniversityChangsha 410082China Department of Orthopaedics Xiangya HospitalCentral South UniversityChangsha 410008China Key Laboratory of Carcinogenesis and Cancer Invasions of Ministry of Education School of Basic Medicine ScienceCancer Research InstituteCentral South UniversityChangsha 410008China

Physical forces,such as magnetic and mechanical stimulation,are known to play a significant role in the regulation of cell *** the present study,a biomimetic regeneration patch was fabricated using E-jet 3 D printing,which integrates mechanical and magnetic stimulation in a biocompatible"one-pot reaction"strategy when combined with a static magnetic field(SMF).The magneto-based therapeutic regeneration patch induced myoblasts to form aligned and multinucleated myotubes,regulated the expression of myogenic-related genes,and activated the p38αmitogen-activated protein kinase pathway via the initiation of myogenic *** validate the efficiency of the proposed strategy,the regeneration patch was implanted into mice and exposed to a suitable SMF,which resulted in significantly enhanced in vivo skeletal muscle *** findings demonstrated that appropriate external physical stimulation provides a suitable biophysical microenvironment that is conducive to tissue *** method used in the present study represents a promising technique to induce the regeneration of damaged skeletal muscle tis sue.

关键词： Functional biomaterials Magnetic actuation Myogenic differentiation skeletal muscle regeneration 3D printing

Wet tissue adhesive polymeric powder hydrogels for skeletal muscle regeneration

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Bioactive Materials 2024年第10期40卷 334-344页

作者： Mingyu Lee Daun Seo Junggeon Park Sun Hong Lee Jin Jeon Woochan Kim Jangho Kim Hee Seok Yang Jae Young Lee School of Materials Science and Engineering Gwangju Institute of Science and TechnologyGwangju61005Republic of Korea Department of Nanobiomedical Science&BK21 FOUR NBM Global Research Center for Regenerative Medicine Dankook UniversityCheonan31116Republic of Korea Department of Convergence Biosystems Engineering Chonnam National UniversityGwangju61186Republic of Korea Department of Rural and Biosystems Engineering Chonnam National UniversityGwangjuRepublic of Korea Interdisciplinary Program in IT-Bio Convergence System Chonnam National UniversityGwangju61186Republic of Korea

Volumetric muscle loss(VML)frequently results from traumatic incidents and can lead to severe functional *** have been widely employed for VML tissue regeneration,which are unfortunately ineffective because of the lack of intimate contact with injured tissue for structural and mechanical *** hydrogels allow for strong tissue connections for wound ***,conventional adhesive hydrogels exhibit poor tissue adhesion in moist,bleeding wounds due to the hydration layer at the tissue–hydrogel interfaces,resulting in insufficient *** this study,we developed a novel,biocompatible,wet tissue adhesive powder hydrogel consisting of dextran-aldehyde(dex-ald)and gelatin for the regeneration of *** powder absorbs the interfacial tissue fluid and buffer solution on the tissue,spontaneously forms a hydrogel,and strongly adheres to the tissue via various molecular interactions,including the Schiff base *** particular,the powder composition with a 1:4 ratio of dex-ald to gelatin exhibited optimal characteristics with an appropriate gelation time(258 s),strong tissue adhesion(14.5 kPa),and ***-ald/gelatin powder hydrogels presented strong adhesion to various organs and excellent hemostasis compared to other wet hydrogels and fibrin glue.A mouse VML injury model revealed that the dex-ald/gelatin powder hydrogel significantly improved muscle regeneration,reduced fibrosis,enhanced vascularization,and decreased ***,our wet-adhesive powder hydrogel can serve as an effective platform for repairing various tissues,including the heart,muscle,and nerve tissues.

关键词： Powder Hydrogel Wet-adhesive skeletal muscle regeneration

SCSMRD: A database for single-cell skeletal muscle regeneration

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Journal of Integrative Agriculture 2023年第3期22卷 864-871页

作者： FENG Xi-kang XIE Chun-di LI Yong-yao WANG Zi-shuai BAI Li-jing School of Software Northwestern Polytechnical UniversityXi’an 710072P.R.China Institute of Animal Sciences Chinese Academy of Agricultural SciencesBeijing 100193P.R.China Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518000P.R.China

skeletal muscle regeneration is a complex process where various cell types and cytokines are ***-cell RNA-sequencing (scRNA-seq) provides the opportunity to deconvolute heterogeneous tissue into individual cells based on their transcriptomic *** scRNA-seq studies on mouse muscle regeneration have provided insights to understand the transcriptional dynamics that underpin muscle ***,a database to investigate gene expression profiling during skeletal muscle regeneration at the single-cell level is ***,we collected over 105 000 cells at 7 key regenerative time-points and non-injured muscles and developed a database,the Singlecell skeletal muscle regeneration Database (SCSMRD).SCSMRD allows users to search the dynamic expression profiles of genes of interest across different cell types during the skeletal muscle regeneration *** also provides a network to show the activity of regulons in different cell types at different time *** analysis showed the state changes trajectory of muscle stem cells (MuSCs) during skeletal muscle *** database is freely available at https://***.

关键词： scRNA-seq skeletal muscle regeneration database regulon network pseudotime

Bioactive biodegradable polycitrate nanoclusters enhances the myoblast differentiation and in vivo skeletal muscle regeneration via p38 MAPK signaling pathway

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Bioactive Materials 2020年第3期5卷 486-495页

作者： Yi Guo Min Wang Juan Ge Wen Niu Mi Chen Wei Cheng Bo Lei Frontier Institute of Science and Technology Xi'an Jiaotong UniversityXi'an710054China Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research College of StomatologyXi'an Jiaotong UniversityXi'an710054China National and Local Joint Engineering Research Center of Biodiagnosis and Biotherapy The Second Affiliated Hospital of Xi'an Jiaotong UniversityXi'an710000China Instrument Analysis Center Xi'an Jiaotong UniversityXi'an710054China Department of Biomedical Engineering University of MichiganAnn ArborMI48109United States

Complete skeletal muscle repair and regeneration due to severe large injury or disease is still a *** cues are critical to control myoblast cell function and can be utilized to develop smart biomaterials for skeletal muscle *** acid-based biodegradable polymers have received much attention on tissue engineering,however,their regulation on myoblast cell differentiation and mechanism was few ***,we find that citrate-based polycitrate-polyethylene glycol-polyethylenimine(POCG-PEI600)nanoclusters can significantly enhance the in vitro myoblast proliferation by probably reinforcing the mitochondrial number,promote the myotube formation and full-thickness skeletal muscle regeneration in vivo by activating the myogenic biomarker genes expression of Myod and ***-PEI600 nanoclusters could also promote the phosphorylation of p38 in MAP kinases(MAPK)signaling pathway,which led to the promotion of the myoblast *** in vivo skeletal muscle loss rat model also confirmed that POCG-PEI600 nanoclusters could significantly improve the angiogenesis,myofibers formation and complete skeletal muscle ***-PEI600 nanocluster could be also biodegraded into small molecules and eliminated in vivo,suggesting their high biocompatibility and *** study could provide a bioactive biomaterial-based strategy to repair and regenerate skeletal muscle tissue.

关键词： Bioactive biomaterials Citrate nanopolymer Myoblast differentiation skeletal muscle regeneration

Function and regulation of muscle stem cells in skeletal muscle development and regeneration:a narrative review

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Journal of Bio-X Research 2021年第3期4卷 89-96页

作者： Han Zhu Xin Lin Yarui Diao Departments of Pediatrics and Cellular&Molecular Medicine Pediatric Diabetes Research CenterSanford Consortium for Regenerative MedicineUniversity of CalformiaSan DiegoLa JollaCA Department of Cell Biology Duke University Medical CenterDurhamNCUSA regeneration Next Iitiative Duke University Medical CenterDurhamNCUSA Department of Orthopedic Surgery Duke University Medical CenterDurhamNCUSA

skeletal muscle plays an essential role in generating the mechanical force necessary to support the movement of our body and daily exercise. Compared with cardiac and smooth muscle, in mammals, skeletal muscle exhibits remarkable regenerative capacity in response to damage. muscle stem cells, also known as satellite cells, directly contribute to regeneration. Here, we review primary and secondary myogenesis processes with a focus on muscle stem cells, as well as the function and regulation of muscle stem cells in adult muscle regeneration in mammals.

关键词： muscle stem cell satellite cell skeletal muscle development skeletal muscle regeneration stem cell niche

Fast photocurable thiol-ene elastomers with tunable biodegradability,mechanical and surface properties enhance myoblast differentiation and contractile function

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Bioactive Materials 2021年第7期6卷 2120-2133页

作者： Mohamed Alaa Mohamed Aref Shahini Nika Rajabian Julia Caserto Ahmed M.A.El-Sokkary Magda A.Akl Stelios T.Andreadis Chong Cheng Department of Chemical and Biological Engineering University at BuffaloThe State University of New YorkBuffaloNY14260USA Chemistry Department College of ScienceMansoura UniversityMansoura35516Egypt Department of Biomedical Engineering University at BuffaloThe State University of New YorkBuffaloNY14260USA Center of Excellence in Bioinformatics and Life Sciences BuffaloNY14263USA

Biodegradable elastomers are important emerging biomaterials for biomedical applications,particularly in the area of soft-tissue engineering in which scaffolds need to match the physicochemical properties of native ***,we report novel fast photocurable elastomers with readily tunable mechanical properties,surface wettability,and *** elastomers are prepared by a 5-min UV-irradiation of thiol-ene reaction systems of glycerol tripentenoate(GTP;a triene)or the combination of GTP and 4-pentenyl 4-pentenoate(PP;a diene)with a carefully chosen series of di-or *** the subsequent application study,these elastomers were found to be capable of overcoming delamination of myotubes,a technical bottleneck limiting the in vitro growth of mature functional *** glycerol-based elastomers supported the proliferation of mouse and human myoblasts,as well as myogenic differentiation into contractile *** notably,while beating mouse myotubes detached from conventional tissue culture plates,they remain adherent on the elastomer *** results suggest that these elastomers as novel biomaterials may provide a promising platform for engineering functional soft tissues with potential applications in regenerative medicine or pharmacological testing.

关键词： Biodegradable elastomers Cell culture substrate skeletal muscle regeneration Thiol-ene synthesis Tissue engineering

Immunomodulatory extracellular matrix hydrogel induces tissue regeneration in a model of partial glossectomy

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Bioactive Materials 2024年第8期38卷 528-539页

作者： Emma I.Zelus Aaron Panduro Isha Deshmukh Jacqueline Grime Marianna Alperin Andrew M.Vahabzadeh-Hagh Karen L.Christman Shu Chien-Gene Lay Department of Bioengineering UC San Diego9500 Gilman Dr.MC 0412La JollaCA92093-0412USA Department of Obstetrics Gynecologyand Reproductive SciencesDivision of Female Pelvic Medicine and Reconstructive SurgeryUC San Diego School of Medicine9300 Campus PointMC 7433La JollaCA92037-7433USA Department of Otolaryngology-Head&Neck Surgery UC San Diego School of Medicine9300 Campus PointMC 7400La JollaCA92037-7400USA Sanford Consortium for Regenerative Medicine 2880 Torrey Pines Scenic DriveLa JollaCA92037USA Sanford Stem Cell Institute 2880 Torrey Pines Scenic DriveLa JollaCA92037USA

While oropharyngeal cancer treatment regimens,including surgical resection,irradiation,and chemotherapy,are effective at removing tumors,they lead to muscle atrophy,denervation,and fibrosis,contributing to the pathogenesis of oropharyngeal dysphagia-difficulty *** standard of care of rehabilitative tongue strengthening and swallowing exercises is ***,we evaluate an alternative approach utilizing an acellular and injectable biomaterial to preserve muscle content and reduce fibrosis of the tongue after *** muscle extracellular matrix(SKM)hydrogel is fabricated from decellularized porcine skeletal muscle tissue.A partial glossectomy injury in the rat is used to induce tongue fibrosis,and SKM hydrogels along with saline controls are injected into the site of scarring two weeks after *** are harvested at 3 and 7 days post-injection for gene expression and immunohistochemical analyses,and at 4 weeks post-injection to evaluate histomorphological *** hydrogel reduces scar formation and improves muscle regeneration at the site of injury compared to *** additionally modulates the immune response towards an anti-inflammatory *** study demonstrates the immunomodulatory and tissue-regenerative capacity of an acellular and minimally invasive ECM hydrogel in a rodent model of tongue injury.

关键词： Extracellular matrix Biomaterial Hydrogel skeletal muscle regeneration Partial glossectomy Dysphagia Immunomodulation

Regulation of muscle stem cell fate

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Cell regeneration 2022年第1期11卷 413-425页

作者： Xin Fu Cheng-le Zhuang Ping Hu Spine Center Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200092China Colorectal Cancer Center/Department of Gastrointestinal Surgery Shanghai Tenth People’s Hospital Affiliated to Tongji UniversityShanghai 200072China Guangzhou Laboratory Guanghzou International Bio LslandNo.9 XingDaoHuan RoadGuangzhou 510005China Institute for Stem Cell and regeneration Chinese Academy of SciencesBeijing 100101China

skeletal muscle plays a critical role in human *** stem cells(MuSCs)serve as the major cell type contribut-ing to muscle regeneration by directly differentiating to mature muscle *** usually remain quiescent with occasionally self-renewal and are activated to enter cell cycle for proliferation followed by differentiation upon muscle injury or under pathological *** quiescence maintenance,activation,proliferation,and differentiation of MuSCs are tightly *** MuSC cell-intrinsic regulatory network and the microenvironments work coordi-nately to orchestrate the fate transition of *** heterogeneity of MuSCs further complicates the regulation of *** review briefly summarizes the current progress on the heterogeneity of MuSCs and the microenviron-ments,epigenetic,and transcription regulations of MuSCs.

关键词： muscle stem cells skeletal muscle regeneration Asymmetric division MuSC heterogeneity Microenvironments Transcription regulation

Scaffold tissue engineering strategies for volumetric muscle loss

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Plastic and Aesthetic Research 2023年第1期10卷 96-112页

作者： Christina Zhu Karina Sklyar Mehran Karvar Yori Endo Indranil Sinha Division of Plastic Surgery Brigham and Women’s HospitalHarvard Medical SchoolBostonMA 02115USA Texas Tech University Health Sciences Center School of Medicine LubbockTX 79430USA

Volumetric muscle loss(VML)refers to a composite,en bloc loss of skeletal muscle mass resulting in functional *** injuries normally heal with excessive fibrosis,minimal skeletal muscle regeneration,and poor functional *** muscle transfer is a treatment option for some patients but is limited both by the degree of functional restoration as well as donor site *** such,new therapeutic options are *** novo regeneration of skeletal muscle,by way of tissue engineering,is an emerging strategy to treat *** review evaluates available scaffolds for promoting skeletal muscle regeneration and functional recovery following *** use of growth factors and stem cell therapies,which may augment scaffold integration and skeletal muscle reconstitution,are also *** medicine with the use of scaffolds is a promising area in skeletal muscle reconstruction and VML treatment.

关键词： Volumetric muscle loss tissue engineering scaffolds skeletal muscle regeneration