Immunomodulatory extracellular matrix hydrogel induces tissue regeneration in a model of partial glossectomy

作     者：Emma I.Zelus Aaron Panduro Isha Deshmukh Jacqueline Grime Marianna Alperin Andrew M.Vahabzadeh-Hagh Karen L.Christman 

作者机构：Shu Chien-Gene Lay Department of BioengineeringUC San Diego9500 Gilman Dr.MC 0412La JollaCA92093-0412USA Department of ObstetricsGynecologyand Reproductive SciencesDivision of Female Pelvic Medicine and Reconstructive SurgeryUC San Diego School of Medicine9300 Campus PointMC 7433La JollaCA92037-7433USA Department of Otolaryngology-Head&Neck SurgeryUC San Diego School of Medicine9300 Campus PointMC 7400La JollaCA92037-7400USA Sanford Consortium for Regenerative Medicine2880 Torrey Pines Scenic DriveLa JollaCA92037USA Sanford Stem Cell Institute2880 Torrey Pines Scenic DriveLa JollaCA92037USA 

出 版 物：《Bioactive Materials》 (生物活性材料（英文）)

年 卷 期：2024年第38卷第8期

页      面：528-539页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Funding provided by the UCSD Galvanizing Engineering in Medicine Program.The authors would like to thank the UC San Diego Stem Cell Genomics Core at the Sanford Consortium for Regenerative Medicine for assistance with RNA sequencing and analysis 

主　　题：Extracellular matrix Biomaterial Hydrogel Skeletal muscle regeneration Partial glossectomy Dysphagia Immunomodulation 

摘      要：While oropharyngeal cancer treatment regimens,including surgical resection,irradiation,and chemotherapy,are effective at removing tumors,they lead to muscle atrophy,denervation,and fibrosis,contributing to the pathogenesis of oropharyngeal dysphagia-difficulty *** standard of care of rehabilitative tongue strengthening and swallowing exercises is ***,we evaluate an alternative approach utilizing an acellular and injectable biomaterial to preserve muscle content and reduce fibrosis of the tongue after *** muscle extracellular matrix(SKM)hydrogel is fabricated from decellularized porcine skeletal muscle tissue.A partial glossectomy injury in the rat is used to induce tongue fibrosis,and SKM hydrogels along with saline controls are injected into the site of scarring two weeks after *** are harvested at 3 and 7 days post-injection for gene expression and immunohistochemical analyses,and at 4 weeks post-injection to evaluate histomorphological *** hydrogel reduces scar formation and improves muscle regeneration at the site of injury compared to *** additionally modulates the immune response towards an anti-inflammatory *** study demonstrates the immunomodulatory and tissue-regenerative capacity of an acellular and minimally invasive ECM hydrogel in a rodent model of tongue injury.