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Low skeletal muscle mass is associated with non-alcoholic fatty liver disease in Korean adults： the Fifth Korea National Health and Nutrition Examination Survey

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Hepatobiliary & Pancreatic Diseases International 2016年第1期15卷 39-47页

作者： hee yeon kim chang wook kim chung-hwa park jong young choi kyungdo han anwar t merchant yong-moon park division of hepatology department of internal medicinecollege of medicinethe catholic university of korea222Banpo-daeroSeocho-guSeou1 06591Republic of Korea department of biostatistics college of medicinethe catholic university of korea222Banpo-daeroSeocho-guSeou1 06591Republic of Korea department of epidemiology and biostatistics arnold school of public healthuniversity of south carolinaColumbiaSC 29208USA epidemiology branch national institute of environmental health sciencesnational institutes of healthdepartment of health and human servicesResearch Triangle ParkNC 27709USA

BACKGROUND： Sarcopenia and non-alcoholic fatty liver dis- ease （NAFLD） share similar pathophysiological mechanisms, and the relationship between sarcopenia and NAFLD has been recently investigated. The study investigated whether low skel- etal muscle mass is differentially associated with NAFLD by gender in Korean adults. METHODS： We conducted a cross-sectional analysis of the data from the Fifth Korea National Health and Nutrition Examination Survey. The skeletal muscle index （SMI） was obtained by the appendicular skeletal muscle mass divided by the weight. NAFLD was defined as a fatty liver index （FLI）〉60 in the absence of other chronic liver disease. RESULTS： Among the included subjects, 18.3% （SE： 1.4%） in men and 7.0% （SE： 0.7%） in women were classified as having FLI-defined NAFLD. Most of the risk factors for FLI-defined NAFLD showed a significant negative correlation with the SMI in both genders. Multiple logistic regression analysis showed that low SMI was associated with FLI-defined NAFLD, inde- pendent of other metabolic and lifestyle parameters in both genders [males： odds ratio （OR）=1.35; 95% confidence inter- val （CI）： 1.17-1.54; females： OR=1.36; 95% CI： 1.18-1.55]. The magnitude of the association between FLI-defined NAFLD and low SMI was higher in middle aged to elderly males （OR-1.50; 95% CI： 1.22-1.84） than in males less than 45 years of age （OR=1.25; 95% CI： 1.02-1.52） and in premenopausal females （OR=l.50; 95% CI： 1.12-2.03） than in postmenopausal females （OR-1.36; 95% CI： 1.20-1.54）.CONCLUSIONS： Low SMI is associated with the risk of FLI- defined NAFLD independent of other well-known metabolic risk factors in both genders. This association may differ ac- cording to age group or menopausal status. Further studies are warranted to confirm this relationship.

关键词： Korea National Health and Nutrition Examination Survey non-alcoholic fatty liver disease sarcopenia skeletal muscle

Heat shock protein 90 is a potential therapeutic target for ameliorating skeletal muscle abnormalities in Parkinson's disease

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Neural Regeneration Research 2014年第6期9卷 616-621页

作者： Nour Erekat Ahed Al-Khatib Muhammed Al-Jarrah Department of Anatomy Faculty of Medicine Jordan University of Science and Technology (JUST) Department of Pathology Faculty of Medicine Jordan University of Science and Technology Department of Rehabilitation Sciences Faculty of Applied Medical Sciences Jordan University of Science and Technology Fatima College of Health Sciences (FCHS)

Previous studies have confirmed that heat shock protein 90 overexpression can lead to dopami- nergic neuronal death. This study was designed to further investigate what effects are produced by heat shock protein 90 after endurance exercise training. Immunohistochemistry results showed that exercise training significantly inhibited heat shock protein 90 overexpression in the soleus and gastrocnemius in Parkinson＇s disease rats, which is a potential therapeutic target for ameliorating skeletal muscle abnormalities in Parkinso^s disease.

关键词： nerve regeneration heat shock protein 90 Parkinson＇s disease exerecise soleus muscle gastrocnemius muscle skeletal muscle treadmill excise neural regeneration

Longitudinal epitranscriptome profiling reveals the crucial role of N^(6)-methyladenosine methylation in porcine prenatal skeletal muscle development

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Journal of Genetics and Genomics 2020年第8期47卷 465-476页

作者： Xinxin Zhang Yilong Yao Jinghua Han Yalan Yang Yun Chen Zhonglin Tang Fei Gao Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen518124China Comparative Pediatrics and Nutrition Department of Veterinary and Animal SciencesFaculty of Health and Medical SciencesUniversity of CopenhagenFrederiksbergDK-2000Denmark Institute of Animal Sciences Chinese Academy of Agricultural SciencesBeijing100193China

N6-methyladenosine(m6A)represents the most abundantly occurring m RNA modification and is involved in the regulation of skeletal muscle development.However,the status and function of m6A methylation in prenatal myogenesis remains unclear.In this study,we first demonstrated that knockdown of METTL14,an m6A methyltransferase,inhibited the differentiation and promoted the proliferation of C2 C12 myoblast cells.Then,using a refined m6A-specific methylated RNA immunoprecipitation(RIP)with next generation sequencing(Me RIP-seq)method that is optimal for use with samples containing small amounts of RNA,we performed transcriptome-wide m6A profiling for six prenatal skeletal muscle developmental stages spanning two important waves of porcine myogenesis.The results revealed that,along with a continuous decrease in the m RNA expression of the m6A reader protein insulin-like growth factor 2 m RNA-binding protein 1(IGF2 BP1),the m6A methylome underwent highly dynamic changes across different development stages,with most of the affected genes being enriched in pathways related to skeletal muscle development.RNA immunoprecipitation confirmed that IGF2 BP1 targets 76 genes involved in pathways associated with muscle development,including the key marker genes MYH2 and Myo G.Moreover,small interfering RNA(si RNA)-mediated knockdown of IGF2 BP1 induced phenotypic changes in C2C12 myoblasts similar to those observed with knockdown of METTL14.In conclusion,we clarified the dynamics of m6A methylation and identified key genes involved in the regulatory network of porcine skeletal muscle development.

关键词： N^(6)-methyladenosine IGF2BP1 Myoblast skeletal muscle

MicroRNAs Involved in skeletal muscle Differentiation

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Journal of Genetics and Genomics 2013年第3期40卷 107-116页

作者： Wen Luo Qinghua Nie Xiquan Zhang Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding and Key Lab of Chicken Genetics Breeding and Reproduction Ministry ofAgriculture South China Agricultural University

MicroRNAs （miRNAs） negatively regulate gene expression by promoting degradation of target mRNAs or inhibiting their translation. Previous studies have expanded our understanding that miRNAs play an important role in myogenesis and have a big impact on muscle mass, muscle fiber type and muscle-related diseases. The muscle-specific miRNAs, miR-206, miR-1 and miR-133, are among the most studied and best characterized miRNAs in skeletal muscle differentiation. They have a profound influence on multiple muscle differ- entiation processes, such as alternative splicing, DNA synthesis, and cell apoptosis. Many non-muscle-specific miRNAs are also required for the differentiation of muscle through interaction with myogenic factors. Studying the regulatory mechanisms of these miRNAs in muscle differentiation will extend our knowledge of miRNAs in muscle biology and will improve our understanding of the myogenesis regulation.

关键词： MicroRNAs skeletal muscle Differentiation Regulation

Perlecan and synaptophysin changes in denervated skeletal muscle

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Neural Regeneration Research 2012年第17期7卷 1293-1298页

作者： Kai Ma Zhifeng Huang Jianfeng Ma Longquan Shao Huiming Wang Yanliang Wang Department of Oral and Maxillofacial Surgery Affiliated Hospital of Stomatology Medical College of Zhejiang University Hangzhou 310006 Zhejiang Province China Department of Oral and Maxillofacial Surgery Affiliated Hospital of Stomatology Wenzhou Medical College Wenzhou 325027 Zhejiang Province China School of Pharmacy Wenzhou Medical College Wenzhou 325000 Zhejiang Province China Department of Stomatology Nanfang Hospital Southern Medical University Guangzhou 510515 Guangdong Province China

The present study observed sciatic nerve and gastrocnemius muscle changes in denervated rats using morphology methods, and assessed expression of perlecan, an extracellular matrix component, which is located at the skeletal muscle cell surface as acetylcholine esterase, as well as synaptophysin, a synaptic marker. Results showed degeneration and inflammation following transection of the sciatic nerve. In addition, the sciatic nerve-dominated skeletal muscle degenerated with mild inflammation, indicating that skeletal muscle atrophy primarily contributed to denervation-induced nutritional disturbances. With prolonged injury time （1-4 weeks post-injury）, perlecan expression gradually decreased and reached the lowest level at 4 weeks, but synaptophysin expression remained unchanged after denervation. Results suggested that perlecan expression was more sensitive to denervation and reflected regional extracellular matrix changes following denervation.

关键词： perlecan synaptophysin extracellular matdx acetylcholine esterase neuromuscular junction denervated skeletal muscle sciatic nerve neural regeneration

Identification and characterization of chondrogenic progenitor cells in the fascia of postnatal skeletal muscle

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分子细胞生物学报:英文版 2011年第6期3卷 369-377页

作者： Guangheng Li Bo Zheng Laura B.Meszaros Joseph B.Vella Arvydas Usas Tomoyuki Matsumoto Johnny Huard Stem Cell Research Center Department of Orthopaedic SurgeryUniversity of Pittsburgh450 Technology Drive Suite 206PittsburghPA 15219USA Pediatric Cancer Biological Program Department of PediatricsOregon Health and Science UniversityPortlandOR 97239USA Department of Bioengineering University of PittsburghPittsburghPA 15219USA Department of Molecular Genetics and Biochemistry University of PittsburghPittsburghPA 15219USA

Intramuscular injection of bone morphogenetic proteins(BMPs)has been shown to induce ectopic bone formation.A chondrogenic phase is typically observed in this process,which suggests that there may exist a chondrogenic subpopulation of cells residing in skeletal muscle.Two prospective cell populations were isolated from rat skeletal muscle:fascia-derived cells(FDCs),extracted from gluteus maximus muscle fascia(epimysium)and muscle-derived cells(MDCs)isolated from the muscle body.Both populations were investigated for their cell surface marker profiles(flowcytometry analysis),proliferation rates as well as their myogenic and chondrogenic potentials.The majority of FDCs expressed mesenchymal stromal cell markers but not endothelial cell markers.FDCs underwent chondrogenic differentiation after BMP4 treatment in vitro,but not myogenic differentiation.Although MDCs showed chondrogenic potential,they expressed the myogenic cell marker desmin and readily underwent myogenic differentiation in vitro;however,the chondrogenic potential of the MDCs is confounded by the presence of FDC-like cells residing in the muscle perimysium and endomysium.To clarify the role of the muscle-derived myogenic cells in chondrogenesis,mixed pellets with varying ratios of FDCs and L6 myoblasts were formed and studied for chondrogenic potential.Our results indicated that the chondrogenic potential of the mixed pellets decreased with the increased ratio of myogenic cells to FDCs supporting the role of FDCs in chondrogenesis.Taken together,our results suggest that non-myogenic cells residing in the fascia of skeletal muscle have a strong chondrogenic potential and may represent a novel donor cell source for cartilage regeneration and repair.

关键词： skeletal muscle fascia chondrocytes cartilage

Addition of tanshinone IIA to UW solution decreases skeletal muscle ischemia-reperfusion injury

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Acta Pharmacologica Sinica 2006年第8期27卷 991-999页

作者： Hong-gang WANG Zhi-yong LI Xiao-lin LIU~2 Department of Orthopaedics & Microsurgery,the First Affiliated Hospital of Sun Yat-Sen University,Guangzhou 510080,China Department of Orthopaedics & Microsurgery the First Affiliated Hospital of Sun Yat-Sen University Guangzhou China

Aim:To investigate whether tanshinone ⅡA could improve the effect of UWsolution for skeletal muscle preservation and to determine the dose range oftanshinone ⅡA providing optimal protection during ischemia and reperfusion.Methods:Ischemic rat limbs were perfused with UW solution or UW plus tanshinoneⅡA (UW+T,0.05,0.1,or 0.2 mg/mL) for 0.5h before reperfusion;controls (I/R)received no perfusion.Serum creatine phosphokinase (CPK),aspartate aminotrans-ferase (AST),and lactate dehydrogenase (LDH) were measured pre-ischemia andafter reperfusion (2-h,4-h,and 6-h).muscle water content,superoxide dismutase(SOD),malondialdehyde (MDA),adenosine triphosphatase (ATPase) were as-sessed pre-reperfusion and after 6-h reperfusion.Intercellular adhesion molecule-1(ICAM-1) and apoptosis were detected after 6-h reperfusion.Reperfusion bloodflow was monitored during reperfusion period.Results:UW and UW+T pre-vented luxury perfusion during reperfusion and inhibited ICAM-1 expression andapoptosis after 6-h reperfusion.Serum CPK,AST,and LDH levels in UW ratswere significantly less than those in controls after 2-h reperfusion (no difference,4-h or 6-h reperfusion).After 4-h ischemia,there were significant differences inwater content,MDA,SOD,and ATPase between UW and controls,but no differ-ence after 6-h reperfusion.All tests with UW+T rats were significantly differentfrom control results at corresponding durations.Higher tanshinone doses im-proved results.Conclusion:UW plus tanshinone ⅡA increased protection againstI/R injury,suggesting that tanshinone ⅡA has clinical value.

关键词： UW solution tanshinone IIA ischemia-reperfusion skeletal muscle

Co-expression network analysis predicts a key role of microRNAs in the adaptation of the porcine skeletal muscle to nutrient supply

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Journal of Animal Science and Biotechnology 2020年第2期11卷 327-347页

作者： Emilio Mármol-Sánchez Yuliaxis Ramayo-Caldas Raquel Quintanilla TainãFigueiredo Cardoso Rayner González-Prendes Joan Tibau Marcel Amills Centre for Research in Agricultural Genomics(CRAG) CSIC-IRTA-UAB-UBUniversitat Autònoma de Barcelona08193 BellaterraSpain Animal Breeding and Genetics Program Institute for Research and Technology in Food and Agriculture(IRTA)Torre Marimon08140 Caldes de MontbuiSpain Present address:Embrapa Pecuária Sudeste Empresa Brasileira de Pesquisa Agropecuária(EMBRAPA)São CarlosSP 13560-970Brazil Department of Animal Science Universitat de Lleida-Agrotecnio Center25198 LleidaSpain Departament de Ciència Animal i dels Aliments Universitat Autònoma de Barcelona08193 BellaterraBarcelonaSpain

Background:The role of non-coding RNAs in the porcine muscle metabolism is poorly understood,with few studies investigating their expression patterns in response to nutrient supply.Therefore,we aimed to investigate the changes in microRNAs(miRNAs),long intergenic non-coding RNAs(lincRNAs)and mRNAs muscle expression before and after food intake.Results:We measured the miRNA,lincRNA and mRNA expression levels in the gluteus medius muscle of 12 gilts in a fasting condition(AL-T0)and 24 gilts fed ad libitum during either 5 h.(AL-T1,N=12)or 7 h.(AL-T2,N=12)prior to slaughter.The small RNA fraction was extracted from muscle samples retrieved from the 36 gilts and sequenced,whereas lincRNA and mRNA expression data were already available.In terms of mean and variance,the expression profiles of miRNAs and lincRNAs in the porcine muscle were quite different than those of mRNAs.Food intake induced the differential expression of 149(AL-T0/AL-T1)and 435(AL-T0/AL-T2)mRNAs,6(AL-T0/AL-T1)and 28(AL-T0/AL-T2)miRNAs and none lincRNAs,while the number of differentially dispersed genes was much lower.Among the set of differentially expressed miRNAs,we identified ssc-miR-148a-3p,ssc-miR-22-3p and ssc-miR-1,which play key roles in the regulation of glucose and lipid metabolism.Besides,co-expression network analyses revealed several miRNAs that putatively interact with mRNAs playing key metabolic roles and that also showed differential expression before and after feeding.One case example was represented by seven miRNAs(ssc-miR-148a-3p,ssc-miR-151-3p,ssc-miR-30a-3p,ssc-miR-30e-3p,ssc-miR-421-5p,ssc-miR-493-5p and ssc-miR-503)which putatively interact with the PDK4 mRNA,one of the master regulators of glucose utilization and fatty acid oxidation.Conclusions:As a whole,our results evidence that microRNAs are likely to play an important role in the porcine skeletal muscle metabolic adaptation to nutrient availability.

关键词： Co-expression analysis lincRNAs microRNAs Pig Regulatory impact factor skeletal muscle

Mitochondrial Ca^(2+) uptake in skeletal muscle health and disease

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Science China(Life Sciences) 2016年第8期59卷 770-776页

作者： Jingsong Zhou Kamal Dhakal Jianxun Yi Kansas City University of Medicine and Bioscience Dybedal Research Center

muscle uses Ca^(2+) as a messenger to control contraction and relies on ATP to maintain the intracellular Ca^(2+) homeostasis. Mitochondria are the major sub-cellular organelle of ATP production. With a negative inner membrane potential, mitochondria take up Ca^(2+) from their surroundings, a process called mitochondrial Ca^(2+) uptake. Under physiological conditions, Ca^(2+) uptake into mitochondria promotes ATP production. Excessive uptake causes mitochondrial Ca^(2+) overload, which activates downstream adverse responses leading to cell dysfunction. Moreover, mitochondrial Ca^(2+) uptake could shape spatio-temporal patterns of intracellular Ca^(2+) signaling. Malfunction of mitochondrial Ca^(2+) uptake is implicated in muscle degeneration. Unlike non-excitable cells, mitochondria in muscle cells experience dramatic changes of intracellular Ca^(2+) levels. Besides the sudden elevation of Ca^(2+) level induced by action potentials, Ca^(2+) transients in muscle cells can be as short as a few milliseconds during a single twitch or as long as minutes during tetanic contraction, which raises the question whether mitochondrial Ca^(2+) uptake is fast and big enough to shape intracellular Ca^(2+) signaling during excitation-contraction coupling and creates technical challenges for quantification of the dynamic changes of Ca^(2+) inside mitochondria. This review focuses on characterization of mitochondrial Ca^(2+) uptake in skeletal muscle and its role in muscle physiology and diseases.

关键词： skeletal muscle mitochondria Ca2+