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Nanopore-based sensing interface for single molecule electrochemistry

Science China Chemistry 2019年第12期62卷 1576-1587页

作者： Rui Gao Yao Lin Yi-Lun Ying Yi-Tao Long Department of Chemistry University of UtahSalt Lake CityUtah 84112USA School of Chemistry&Molecular Engineering East China University of Science and TechnologyShanghai 200237China State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210023China

Nanopore techniques are experiencing a gallop since it walked out the notebook and show its charm on the science *** nanoscale pore offers a single-molecule resolution with a label-free and high-selective manner for the research of molecular structures,molecular dynamics,single-molecule reactions and for a variety of applications in biophysics and *** this review,we introduce the construction of three types of nanopore platforms along with the latest progress in DNA sensing,structure and dynamics analysis of peptides/proteins,and the detection of redox reactions with new sensing ***,we depict nanopore data processing methods which provide an insight of data mining under the background ofbig *** could fully expect the great impact of nanopore techniques on not only for DNA sequencing and sensing applications,but also in protein sequencing and clinical diagnostics.

关键词： nanopore single-molecule sensing nanoelectrochemistry

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Localised solid-state nanopore fabrication via controlled breakdown using on-chip electrodes

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Nano Research 2022年第11期15卷 9881-9889页

作者： Jasper P.Fried Jacob L.Swett Binoy Paulose Nadappuram Aleksandra Fedosyuk Alex Gee Ondrej E.Dyck James R.Yates Aleksandar P.Ivanov Joshua B.Edel Jan A.Mol Department of Materials University of OxfordOxfordOX13PHUK School of Chemistry University of New South WalesSydneyNew South Wales 2052Australia Department of Chemistry Imperial College LondonLondonW120BZUK Center for Nanophase Materials Sciences Oak Ridge National LaboratoryOak RidgeTN 37830USA Instituto de Tecnologia Química e Biológica António Xavier Universidade Nova de LisboaAv.da RepúblicaOeiras 2780-157Portugal School of Physics and Astronomy Queen Mary University of LondonLondonE14NSUK

Controlled breakdown has recently emerged as a highly accessible technique to fabricate solid-state ***,in its most common form,controlled breakdown creates a single nanopore at an arbitrary location in the ***,we introduce a new strategy whereby breakdown is performed by applying the electric field between an on-chip electrode and an electrolyte solution in contact with the opposite side of the *** demonstrate two advantages of this ***,we can independently fabricate multiple nanopores at given positions in the membrane by localising the applied field to the ***,we can create nanopores that are self-aligned with complementary nanoelectrodes by applying voltages to the on-chip electrodes to locally heat the membrane during controlled *** new controlled breakdown method provides a path towards the affordable,rapid,and automatable fabrication of arrays of nanopores self-aligned with complementary on-chip nanostructures.

关键词： solid-state nanopores dielectric breakdown nanofabrication single-molecule sensing nanopore arrays

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Challenging Nanopores with Analyte Scope and Environment

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Journal of Analysis and Testing 2019年第1期3卷 61-79页

作者： Buddini I.Karawdeniya Y.M.Nuwan D.Y.Bandara Jonathan W.Nichols Robert B.Chevalier James T.Hagan Jason R.Dwyer Department of Chemistry University of Rhode IslandRichard E.Beaupre Center for Chemical and Forensic Sciences140 Flagg Rd.KingstonRI 02881USA Present Address:Department of Mechanical Engineering Lyle School of EngineeringSouthern Methodist UniversityDallasTX 75275USA Present Address:Nova Biomedical 200 Prospect StreetWalthamMA 02454-9141USA

Nanopores are nanofluidic channels formed through thin membranes that can deliver standout single-molecule and single-particle sensing *** targets include small molecules and nanoparticles,and the DNA,protein,and glycan biopolymers underpinning genomics,proteomics,and ***-notably even in the simplest implementation,resistive-pulse sensing-does not inherently require sample labeling and,thus,offers the potential for general sensing util-ity combined with the prospective benefits of reduced sample processing requirements.A key pursuit for biopolymer sens-ing is the characterization of monomer *** review article will provide an overview of the use of nanopores for general chemical sensing and-omics-related applications,*** broad analyte scope provides fertile ground for a discussion of principles governing nanopore sensing and considerations useful for guiding nanopore *** nanopores to be effective in the face of broad analyte scope,stringent requirements on analytical performance must be met within the particular analyte class without sacrificing the operational flexibility necessary to be responsive across classes presenting very different physical and chemical *** sample-driven challenges provide a unifying framework for discussing aspects of nanopore fabrication,properties,and integration;sensing paradigms,performance,and prospects;fundamental electrokinetic and interfacial phenomena;and practical challenges facing the use and further development of nanopore devices.

关键词： Nanopore single-molecule sensing Nanofluidics Point-of-care Silicon nitride Resistive pulse Wearable sensors Quality assurance

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