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Transcriptome sequencing and metabolome analysis reveal the mechanism of shuanghua baihe tablet in the treatment of oral mucositis

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Chinese Journal of Natural Medicines 2021年第12期19卷 930-943页

作者： GENG Qi-Shun LIU Rui-Juan SHEN Zhi-Bo WEI Qian ZHENG Yuan-Yuan JIA Lan-Qi WANG Long-Hao LI Li-Feng LI Jun XUE Wen-Hua Department of Pharmacy the First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052China Engineering Laboratory for Digital Telemedicine Service The first Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052China

Oral mucositis(OM) caused by cancer therapy is the most common adverse reaction in the radiotherapy of head and neck tumors. In severe cases, it can lead to the interruption of treatment, which affects the control of the disease and the quality of *** baihe tablet(SBT) is a traditional Chinese medicine(TCM) formula, which is administerd to treat OM in China. It has been clinically effective for more than 30 years, but the underlying mechanism is not completely understood. With the development of multiple omics, it is possible to explore the mechanism of Chinese herbal compound prescriptions. Based on transcriptomics and metabolomics, we explored the underlying mechanism of SBT in the treatment of OM. An OM model of rats was established by 5-FU induction, and SBT was orally administered at dosages of 0.75 and 3 g·kg;·d;. In order to search for SBT targets and related metabolites, the dysregulated genes and metabolites were detected by transcriptomics and metabolomics. Immune related indicators such as interleukin-17(IL-17) and tumor necrosis factor-α(TNF-α) were detected by ELISA. Treg cell disorders was analyzed by flow cytometry. Our results showed that SBT significantly alleviated the symptoms of OM rats and the inflammatory infiltration of ulcer *** SBT administration, inflammatory related metabolic pathways including linoleic acid metabolism, valine, leucine and isoleucine biosynthesis were significantly altered. Furthermore, the production of proinflammatory factors like IL-17 and TNF-α, were also dramatically reduced after SBT administration. Besides, the infiltration degree of Treg cells in the spleen of OM modeling rats was significantly improved by SBT administration, thus maintaining the immune balance of the body. The current study demonstrates that SBT regulates inoleic acid metabolism, glycerophospholipid metabolism and amino acid metabolism, and inhibits IL-17/TNF signal transduction to restore Treg and Th17 cell homeostasis in OM

关键词： Oral mucositis shuanghua baihe tablet Transcriptomics Metabolomics IL-17/TNF signal pathway

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