Transcriptome sequencing and metabolome analysis reveal the mechanism of Shuanghua Baihe Tablet in the treatment of oral mucositis
Transcriptome sequencing and metabolome analysis reveal the mechanism of Shuanghua Baihe Tablet in the treatment of oral mucositis作者机构：Department of Pharmacythe First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052China Engineering Laboratory for Digital Telemedicine ServiceThe first Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052China
出 版 物：《Chinese Journal of Natural Medicines》 (中国天然药物（英文版）)
年 卷 期：2021年第19卷第12期
页 面：930-943页
核心收录：
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
基 金：supported by the Clinical Research Special Fund of Wu Jieping Medical Foundation(No.320.6750.2020-04-9) the National Key Research and Development Program of China(No.2017YFC0909900) the National Natural Science Foundation of China(No.82002433) the Science and Technology Project of Henan Provincial Department of Education(Nos.18A320044 and 21A320036) Henan Province Medical Science and Technology Research Project Joint Construction Project(Nos.LHGJ20190003 and LHGJ20190055)
主 题：Oral mucositis Shuanghua Baihe tablet Transcriptomics Metabolomics IL-17/TNF signal pathway
摘 要：Oral mucositis(OM) caused by cancer therapy is the most common adverse reaction in the radiotherapy of head and neck tumors. In severe cases, it can lead to the interruption of treatment, which affects the control of the disease and the quality of *** Baihe Tablet(SBT) is a traditional Chinese medicine(TCM) formula, which is administerd to treat OM in China. It has been clinically effective for more than 30 years, but the underlying mechanism is not completely understood. With the development of multiple omics, it is possible to explore the mechanism of Chinese herbal compound prescriptions. Based on transcriptomics and metabolomics, we explored the underlying mechanism of SBT in the treatment of OM. An OM model of rats was established by 5-FU induction, and SBT was orally administered at dosages of 0.75 and 3 g·kg;·d;. In order to search for SBT targets and related metabolites, the dysregulated genes and metabolites were detected by transcriptomics and metabolomics. Immune related indicators such as interleukin-17(IL-17) and tumor necrosis factor-α(TNF-α) were detected by ELISA. Treg cell disorders was analyzed by flow cytometry. Our results showed that SBT significantly alleviated the symptoms of OM rats and the inflammatory infiltration of ulcer *** SBT administration, inflammatory related metabolic pathways including linoleic acid metabolism, valine, leucine and isoleucine biosynthesis were significantly altered. Furthermore, the production of proinflammatory factors like IL-17 and TNF-α, were also dramatically reduced after SBT administration. Besides, the infiltration degree of Treg cells in the spleen of OM modeling rats was significantly improved by SBT administration, thus maintaining the immune balance of the body. The current study demonstrates that SBT regulates inoleic acid metabolism, glycerophospholipid metabolism and amino acid metabolism, and inhibits IL-17/TNF signal transduction to restore Treg and Th17 cell homeostasis in OM
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