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Establishment of highly efficient plant regeneration of Paeonia ostii‘Fengdan’through optimization of callus,adventitious shoot,and rooting induction

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Horticultural Plant Journal 2022年第6期8卷 777-786页

作者： Rong Liu Yuqian Xue Huiting Ci Jie Gao Shunli Wang Xiuxin Zhang Key Laboratory of Biology and Genetic Improvement of Horticultural Crops Ministry of Agriculture and Rural Affairs ChinaInstitute of Vegetables and FlowersChinese Academy of Agricultural SciencesInstitute of PeonyChinese Academy of Agricultural SciencesBeijing 100081China

Tree peony is a famous ornamental plant,while the low propagation rate is the main hurdles hindering the industry *** now,the highly efficient regeneration system for tree peony is not *** this study,using Paeonia ostii’Fengdan’mature embryos,the effects of variations in inoculation method,initiating culture,adventitious shoot induction,rooting media,plant growth regulators(PGRs),and a nonconventional PGR(plant extracts)on regeneration from explants were *** embryo cultures,embryonic callus induction rate of 1/4 embryos was the highest among those of embryos with other three technical treatments(whole embryos,1/2 embryos,and pieces of embryos).The woody plant medium(WPM)containing 1.0 mg·L^(-1)6-BA,0.5 mg·L^(-1)GA3,30.0 g·L^(-1)sucrose,and 3.0 g·L^(-1)phytagel significantly improved shoot induction and multiplication.3.0 mg·L^(-1)plant extracts promoted hypocotyl germination,rooting,and root growth,in direct embryo culture,and a combination of 3.0 mg·L^(-1)plant extracts+2.0 mg·L^(-1)IBA+1.5 mg·L^(-1)IAA produced optimal rooting induction rate for multiple shoots in direct embryo culture and indirect somatic *** the three in vitro micropropagation methods,the highest shoot proliferation coefficient(5.4±0.2)was obtained with indirect somatic ***,the propagation ability of shoots remains high,even when culture propagation was continued for more than two ***,a reliable system for plant regeneration from mature embryos derived from ***’Fengdan’callus and two direct embryo culture systems have been *** novel regeneration system could facilitate uniform seedling production.

关键词： Tree peony Mature embryos Plant extracts shoot induction Rooting Plant regeneration

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In Vitro Propagation of Three Strawberry Cultivars through Runner Tips Culture

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American Journal of Plant Sciences 2023年第11期14卷 1296-1304页

作者： Sussmita Karmaker Md. Mukhtar Hossain Md. Aminul Hoque Md. Abdul Kaium Md. Al Amin Md. Saidul Islam Department of Agronomy and Agricultural Extension Faculty of Agriculture Rajshahi University Rajshahi Bangladesh Department of Crop Science and Technology Faculty of Agriculture Rajshahi University Rajshahi Bangladesh

At the Genetics and Plant Breeding Laboratory of the Department of Agronomy and Agricultural Extension, University of Rajshahi, Bangladesh, strawberry in vitro propagation was done. Five Benzylaminopurine (BAP) concentrations were utilized for shoot induction—0.0 mg/L (Control), 0.5 mg/L, 1.0 mg/L, 1.5 mg/L, and 2.0 mg/L and five Indole Buteric Acid (IBA) concentrations—0.0 mg/L(Control), 0.5 mg/L, 1.0 mg/L, 1.5 mg/L, and 2.0 mg/l—were used for the induction of the root. The highest mean amount of shoots (eight) and length of the shoot (3.40 cm) were observed at a concentration of BAP of 0.5 mg/L. Festival also had the highest mean amount of leaves (6) when we used the identical concentration, while RABI-3 and Camarosa did the least well. The IBA of 0.5 mg/L concentration of rooting performed the best across all metrics tested among the five concentrations. The longest (3.3 cm) roots and most roots (7) were likewise obtained from this concentration in Festival. However, RABI-3 and Camarosa performed worse than Festival in the same concentration. Half-strength MS media without IBA concentration showed no response regarding root induction for each of the three cultivars.

关键词： Strawberry Proliferation Propagation shoot induction Root induction

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Efficient Evergreen Plant Regeneration of Cinnamomum japonicum *** in vitro Organogenesis

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Phyton-International Journal of Experimental Botany 2021年第2期90卷 571-582页

作者： Jiaji Zhang Xiaofei Long Yuhao Weng Tielong Cheng Jisen Shi Jinhui Chen Key Laboratory of Forest Genetics&Biotechnology of Ministry of Education Co-Innovation Center for Sustainable Forestry in Southern ChinaNanjing Forestry UniversityNanjing210037China College of Biology and the Environment Co-Innovation Center for Sustainable Forestry in Southern ChinaNanjing Forestry UniversityNanjing210037China

Cinnamomum japonicum *** an excellent roadside tree and medicinal tree species with considerable ornamental and economic *** this study,we successfully developed a large-scale micropropagation protocol for *** for the first *** shoots were excised and used as explants for shoot induction on several basal media,supplemented with different concentrations of plant growth regulators(PGRs),such as Thidiazuron(TDZ),N^(6)-Benzyladenine(6-benzylaminopurine)(BA),α-naphthaleneacetic acid(NAA)and Gibberellic acid(GA_(3)).After comparison,the most efficient medium for shoot regeneration was 1/2 Murashige and Skoog(MS)medium containing 0.5 mg L^(-1)BA,0.05 mg L^(-1)NAA and 0.2 mg L^(-1)GA_(3),which resulted in an average number of induced shoots per explant and shoot length of 5.2 and 1.62 cm at 28 d,***,elongated adventitious shoots were transferred to induce roots.86.7%of shoots was able to root on 1/2 MS medium supplemented with 0.5 mg L^(-1)NAA and 0.1 mg L^(-1)*** earliest rooting time observed was after 21 d and the average root length was up to 3.3 cm after 28 *** study shows that *** can be successfully regenerated through de novo organogenesis,which lays a foundation for future transformation research on this tree.

关键词： Cinnamomum japonicum Sieb. micropropagation de novo organogenesis shoot induction rhizogenesis

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