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Assessing the response of indigenous loquat cultivar Mardan to phytohormones for in vitro shoot proliferation and rooting

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第9期14卷 774-784页

作者： Nadeem Akhtar ABBASI Tariq PERVAIZ Ishfaq Ahmed HAFIZ Mehwish YASEEN Azhar HUSSAIN Plant Tissue Culture Laboratory Department of HorticulturePir Mehr Ali Shah-Arid Agriculture University

In vitro cultures of loquat cultivar Mardan were established using shoot apices after treating with NaOCl (5%,7%,10%,12%,14% (v/v)) for 12 min and HgCl2 (0.01%,0.05%,0.10%,0.20%,0.25% (w/v)) for 2 min.A maximum survival rate of 70% was recorded after surface sterilization with 10% *** response was assessed on Murashige and Skoog (MS) medium fortified with assorted combinations of the cytokinins,benzylaminopurine (BAP),kinetin,and N6-(2-isopentyl)adenine (2iP).Treatment of BAP 1.5 mg/L combined with 2iP 9.0 mg/L and kinetin 1.5 mg/L was found to be optimum for shoot morphogenesis in terms of the number and subsequent growth of shoots,while the highest shoot length was yielded by the combination of BAP 0.5 mg/L,kinetin 0.5 mg/L,and 2iP 3 mg/*** levels of cytokinins induced callogenesis,vitrification and stunted growth to some *** rhizogenesis,uniform sized micro-shoots were excised and transferred to half-strength MS medium containing *** best rooting expression was observed with naphthaleneacetic acid (NAA) 1 mg/L combined with indole-3-butyric acid (IBA) 2 mg/L and paclobutrazol (PBZ) 1 mg/L.

关键词： Eriobotryajaponica Micropropagation Sterilization of Ioquat Plant growth regulator shoot proliferation Rhizogenesis

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In Vitro Propagation of Three Strawberry Varieties and Field Evaluation

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American Journal of Plant Sciences 2023年第11期14卷 1214-1222页

作者： Sussmita Karmaker Md. Mukhtar Hossain Md. Aminul Hoque Md. Abdul Kaium Tabaraka Binte Ali Musfika Ferdous Department of Agronomy and Agricultural Extension Faculty of Agriculture Rajshahi University Rajshahi Bangladesh Department of Crop Science and Technology Faculty of Agriculture Rajshahi University Rajshahi Bangladesh

A study was done to produce a rapid in vitro propagation of three strawberry genotypes and tested in the field under Bangladeshi circumstances. Festival, RABI-3, and Neho strawberry genotypes’ runner tips were cultivated in vitro to induce root induction and multiple shoot proliferation. MS (Murashige and Skoog) media that were basally containing three different concentrations at 1.0, 1.5, and 2.0 of BA (6-benzyl adenine), KIN (6-furfuryl amino purine), or GA3 (gibberellic acid) at 0.5 mg/L increasing tips of the runner was attained. The culture grew on the medium provided with 1.5 mg/L 6-benzyl adenine and 0.5 mg/L 6-furfuryl amino acid to increase shoot at the best level. Micro-cuttings were rooted on MS media at half strength combined with 0.5 mg/L - 1.5 mg/L IBA (indole butyric acid) or IAA (indole acetic acid). IBA attained 4 - 9 roots and 91% - 96% rooting at 1.0 mg/L. The resulting plantlets grew into hardy plants and took root in the earth. The genotype festival had the highest response rate, followed by RABI-3 and Neho.

关键词： Strawberry In Vitro Propagation Genotypes Root Induction shoot proliferation

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