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Infection with severe fever with thrombocytopenia virus in healthy population:a cohort study in a high endemic region,China

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Infectious Diseases of Poverty 2021年第6期10卷 74-82页

作者： Xiao‑Lei Ye Ke Dai Qing‑Bin Lu Yan‑Qin Huang Shou‑Ming Lv Pan‑He Zhang Jia‑Chen Li Hai‑Yang Zhang Zhen‑Dong Yang Ning Cui Chun Yuan Kun Liu Xiao‑Ai Zhang Jiu‑Song Zhang Hao Li Yang Yang Li‑Qun Fang Wei Liu State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Micro‑biology and Epidemiology20 Dong‑Da StreetFengtai DistrictBeijing 100071People’s Republic of China Department of Laboratorial Science and Tech‑nology School of Public HealthPeking UniversityBeijing 100191People’s Republic of China Shangcheng Center for Diseases Control and Prevention Xinyang 464000People’s Republic of China The 990 Hospital of Chinese People’s Liberation Army Joint Logistic Support Force Xinyang 464000Peo‑ple’s Republic of China Department of Epidemiology Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environ‑mentSchool of Public HealthAir Force Medical UniversityXi’an 710032People’s Republic of China Department of Biostatistics College of Public Health and Health Professionsand Emerging Pathogens InstituteUniversity of FloridaGainesvilleFL 32611USA

Background: severe fever with thrombocytopenia (SFTS) caused by SFTS virus (SFTSV) was a tick-borne hemorrhagic fever that posed significant threat to human health in Eastern Asia. The study was designed to measure the seroprevalence of SFTSV antibody in healthy population residing in a high endemic ***: A cohort study was performed on healthy residents in Shangcheng County in Xinyang City from April to December in 2018, where the highest SFTS incidence in China was reported. Anti-SFTSV IgG was measured by indirect enzyme-linked immunosorbent assay and neutralizing antibody (NAb) was detected by using PRNT50. The logistic regression models were performed to analyze the variables that were associated with seropositive ***: Totally 886 individuals were recruited. The baseline seroprevalence that was tested before the epidemic season was 11.9% (70/587) for IgG and 6.8% (40/587) for NAb, which was increased to 13.4% (47/350) and 7.7% (27/350) during the epidemic season, and further to 15.8% (80/508) and 9.8% (50/508) post epidemic. The IgG antibody-based seropositivity was significantly related to the patients aged ≥ 70 years old [adjusted odds ratio (OR) = 2.440, 95% confidence interval (CI): 1.334-4.461 compared to the group of < 50 years old,P = 0.004], recent contact with cats (adjustedOR = 2.195, 95%CI: 1.261-3.818,P = 0.005), and working in tea garden (adjustedOR = 1.698, 95%CI: 1.002-2.880,P = 0.049) by applying multivariate logistic regression model. The NAb based seropositivity was similarly related to the patients aged ≥ 70 years old (adjustedOR = 2.691, 95%CI: 1.271-5.695 compared to the group of < 50 years old,P = 0.010), and recent contact with cats (OR = 2.648, 95%CI: 1.419-4.941,P = 0.002). For a cohort of individuals continually sampled with 1-year apart, the anti-SFTSV IgG were maintained at a stable level, while the NAb level ***: Subclinical infection might not provide adequate immunity to protect reinfection of SF

关键词： severe fever with thrombocytopenia Serological study Healthy participant IgG antibody Neutralizing antibody

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Inactivation of severe fever with thrombocytopenia syndrome virus for improved laboratory safety

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Journal of Biosafety and Biosecurity 2020年第1期2卷 31-35页

作者： Toshihiko Harada Shuetsu Fukushi Takeshi Kurosu Tomoki Yoshikawa Masayuki Shimojima Kiyoshi Tanabayashi Masayuki Saijo Division of Biosafety Control and Research National Institute of Infectious DiseasesTokyoJapan Department of Virology 1 National Institute of Infectious DiseasesTokyoJapan

severe fever with thrombocytopenia syndrome(SFTS),a tick-borne infectious disease with high mortality,is diagnosed by the serological testing of serum samples from patients in the acute and convalescent phases and/or by direct detection of the SFTS virus(SFTSV).Conventionally,heat and UV treatments have been used to inactivate the viruses present in serum samples before performing the serological ***,we examined the inactivation conditions optimal for SFTSV-containing serum samples to ensure the safety of laboratory workers while maintaining the accuracy of serological assay results *** human serum samples spiked with SFTSV to 60C for 30 min or exposing them to UV irradiation for 30 min,reduced the infectious virus titer below the limit of *** in sera from patients in the acute phase of SFTS was completely inactivated by heating to 60C for 30 min,followed by UV irradiation for 10 *** inactivation procedure had minimal impact on the performance of SFTSV antibody detection *** data provided herein can serve as a guide for laboratory workers and researchers working with SFTS serological tests.

关键词： Biosafety Inactivation severe fever with thrombocytopenia syndrome(SFTSV) Serology

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