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Chemical comparison of semen euphorbiae and semen euphorbiae Pulveratum by UPLC-Q-TOF/MS coupled with multivariate statistical techniques

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Journal of Chinese Pharmaceutical Sciences 2020年第7期29卷 470-479页

作者： Huinan Wang Jingzhen Zhang Yuexin Cui Siyu Wang Hui Gao Yao Zhang Xinjie Wang Ziye Yang Mengyu Chen Peihua Wang Guimei Zhang Yingzi Wang Chao Zhang School of Chinese Materia Medica Beijing University of Chinese MedicineBeijing102488China School of Pharmaceutical Sciences Shandong University of Traditional Chinese MedicineJinan 250355China

In the present study,we aimed to assess the chemical composition changes of semen euphorbiae(SE)and semen euphorbiae Pulveratum(SEP)by UPLC-Q-TOF/MS and multivariate statistical *** UPLC-Q-TOF/MS method and SIMCA-P software were used to analyze the changes of chemical components of SE and SEP based on PCA and PLS-DA multivariate statistical methods.A"component-target-disease"network model was constructed by Intelligent Platform for Life Sciences of traditional Chinese medicine(TCM)to predict potential related *** differences of chemical composition were significant between SE and *** positive ion mode,the amounts of Euphorbia factor L2,L3,L7a,L8,L9 and lathyrol were obviously decreased after *** negative ion mode,the amounts of aesculetin,bisaesculetin,ingenol and cetylic acid were increased obviously,while Euphorbia factor L1,L4 and L5 were decreased obviously after processing,and the components of euphobiasteroid,aesculetin,lathyrol and linoleic acid among the 14 differentiated compounds were closely related to the SE-related diseases through the"component-target-disease"network ***-Q-TOF/MS technology in combination with multivariate statistical methods had certain advantages in studying the complex changes of chemical composition before and after manufacturing pulveratum of *** provided a basis for clarifying the toxicity-attenuated mechanisms of SE manufacturing pulveratum,and laid the foundation for its further development and utilization.

关键词： semen euphorbiae semen euphorbiae Pulveratum UPLC-Q-TOF/MS Multivariate statistical techniques Chemical constituents Manufacturing pulveratum

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Study on the regulatory effect of liver X receptor in HEK293 cells by six main diterpene esters in semen euphorbiae

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TMR Modern Herbal Medicine 2024年第2期7卷 35-40页

作者： Si-Yuan Ma Fan-Miao Kong Xiao-Tong Wei Jun-Li Zhang Hai-Ting Zhu Xin-Ning Zhang Yu-Feng Hu Ming-Rui Jiang Hui-Nan Wang Yi-Cen Xu Ying-Zi Wang School of Chinese Materia Medica Beijing University of Chinese MedicineBeijing 102488China

Background:To study the effects of the main diterpene esters in Euphorbia factor L_(1),L_(2),L_(3),L_(7a),L_(7b)and L_(8)on the transcriptional activity and protein expression of liver X receptor(LXR).Methods:The effect of the main diterpene ester components in semen euphorbiae on the viability of HEK293 cells were studied by MTT *** LXR-Luc plasmid vector was transfected into HEK293 cells and treated with Euphorbia factor L_(1),L_(2),L_(3),L_(7a),L_(7b)and L_(8)for 24 *** effect of the main diterpene ester components of semen euphorbiae on LXR-Luc luciferase activity was investigated by dual luciferase reporter gene system,and the expression of LXRαprotein was detected by Western ***:Euphorbia factor L_(1),L_(2),L_(3),L_(7a),L_(7b)and L_(8)could significantly reduce the relative luciferase activity(RLU)of LXRα,and the expression level of LXRαprotein was significantly ***:Euphorbia factor L_(1),L_(2),L_(3),L_(7a),L_(7b)and L_(8)can inhibit the expression of LXR protein level,which may be achieved by inhibiting the transcriptional activity of LXR.

关键词： semen euphorbiae diterpene esters HEK293 LXR dual luciferase reporter gene system

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