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Estimating Mass of Harvested Asian seabass Lates calcarifer from Images

World Journal of Engineering and Technology 2018年第3期6卷 15-23页

作者： Dmitry A. Konovalov Alzayat Saleh Jose A. Domingos Ronald D. White Dean R. Jerry College of Science and Engineering James Cook University Townsville Australia James Cook University Singapore Singapore Centre for Sustainable Tropical Fisheries and Aquaculture James Cook University Townsville Australia

Total of 1072 Asian seabass or barramundi (Lates calcarifer) were harvested at two different locations in Queensland, Australia. Each fish was digitally photographed and weighed. A subsample of 200 images (100 from each location) were manually segmented to extract the fish-body area (S in cm2), excluding all fins. After scaling the segmented images to 1mm per pixel, the fish mass values (M in grams) were fitted by a single-factor model (M=aS1.5, a=0.1695 )achieving the coefficient of determination (R2) and the Mean Absolute Relative Error (MARE) of R2=0.9819 and MARE=5.1%, respectively. A segmentation Convolutional Neural Network (CNN) was trained on the 200 hand-segmented images, and then applied to the rest of the available images. The CNN predicted fish-body areas were used to fit the mass-area estimation models: the single-factor model, M=aS1.5, a=0.170, R2=0.9819, MARE=5.1%;and the two-factor model, M= aSb, a=0.124, b=0.155, R2=0.9834, MARE=4.5%.

关键词： Aquaculture Asian seabass Barramundi Lates calcarifer Computer Vision Image Processing Weight Estimation

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LAMP for the rapid diagnosis of iridovirus in aquaculture

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Aquaculture and Fisheries 2022年第2期7卷 158-165页

作者： Yepin Yu Zituo Yang Le Wang Fei Sun May Lee Yanfei Wen Qiwei Qin Gen Hua Yue Temasek Life Sciences Laboratory National University of Singapore1 Research Link117604Singapore Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation College of Marine SciencesSouth China Agricultural UniversityGuangzhou510642China Department of Biological Sciences National University of Singapore14 Science Drive117543Singapore

Iridoviruses are DNA virus and have caused huge economic losses in the aquaculture *** aim of this study was to establish a colorimetric loop-mediated isothermal amplification(LAMP)protocol for the on-site detection of Singapore grouper iridovirus(SGIV).The SGIV-VP61 gene was chosen as the target gene to develop a colorimetric LAMP *** optimized condition of the colorimetric LAMP assay was incubation at 63℃ for 1 *** infected with SGIV could be detected with the color change from yellow into *** sensitivity of the developed assay is 5.66 copies/μL of the viral DNA *** sensitivity was about 1000 times higher than that of conventional PCR while it was slightly lower than the one-step semi-nested PCR assay.A total of 60 DNA samples extracted from the fin tissue of the SGIV-infected Asian seabass were examined for SGIV by colorimetric LAMP,semi-nested PCR and conventional *** results of the colorimetric LAMP assay showed 94.87%agreement with the semi-nested *** addition,the DNA extraction method using NaOH showed a better performance in the colorimetric LAMP *** together,the colorimetric LAMP established was a sensitive,rapid and specific method for the detection of *** was not detected in samples randomly taken from a genetically improved line of the Asian ***,some seabass obtained from the local markets were found to contain ***,the LAMP assay has the potential application in the diagnosis of iridovirus diseases in the aquaculture industry.

关键词： Fish Disease Iridovirus Diagnosis PCR seabass

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