LAMP for the rapid diagnosis of iridovirus in aquaculture
作者机构：Temasek Life Sciences LaboratoryNational University of Singapore1 Research Link117604Singapore Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and ExploitationCollege of Marine SciencesSouth China Agricultural UniversityGuangzhou510642China Department of Biological SciencesNational University of Singapore14 Science Drive117543Singapore
出 版 物：《Aquaculture and Fisheries》 (渔业学报（英文）)
年 卷 期：2022年第7卷第2期
页 面：158-165页
基 金：This study was supported by the National Natural Science Foundation of China(41806161) the internal funds of Temasek Life Sciences Lab,Singapore
主 题：Fish Disease Iridovirus Diagnosis PCR Seabass
摘 要：Iridoviruses are DNA virus and have caused huge economic losses in the aquaculture *** aim of this study was to establish a colorimetric loop-mediated isothermal amplification(LAMP)protocol for the on-site detection of Singapore grouper iridovirus(SGIV).The SGIV-VP61 gene was chosen as the target gene to develop a colorimetric LAMP *** optimized condition of the colorimetric LAMP assay was incubation at 63℃ for 1 *** infected with SGIV could be detected with the color change from yellow into *** sensitivity of the developed assay is 5.66 copies/μL of the viral DNA *** sensitivity was about 1000 times higher than that of conventional PCR while it was slightly lower than the one-step semi-nested PCR assay.A total of 60 DNA samples extracted from the fin tissue of the SGIV-infected Asian seabass were examined for SGIV by colorimetric LAMP,semi-nested PCR and conventional *** results of the colorimetric LAMP assay showed 94.87%agreement with the semi-nested *** addition,the DNA extraction method using NaOH showed a better performance in the colorimetric LAMP *** together,the colorimetric LAMP established was a sensitive,rapid and specific method for the detection of *** was not detected in samples randomly taken from a genetically improved line of the Asian ***,some seabass obtained from the local markets were found to contain ***,the LAMP assay has the potential application in the diagnosis of iridovirus diseases in the aquaculture industry.
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