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Transcriptomic profile of human erythroleukemia cells in response to sargassum fusiforme polysaccharide and its structure analysis

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Chinese Journal of Natural Medicines 2021年第10期19卷 784-795页

作者： DING Hao-Miao FU Rui-Jie XIE Ce WANG Cai-Sheng QIAN Guo-Ying College of Biological and Environmental Sciences Zhejiang Wanli UniversityNingbo 315100China School of Marine Sciences Ningbo UniversityNingbo 315211China

sargassum fusiforme(S.fusiforme)has been used as an ingredient in Chinese herbal medicine for thousands of years.However,there are a limited number of studies concerning its therapeutic mechanism.High performance gel permeation chromatography(HPGPC)analysis showed that the average molecular weight of the S.fusiforme polysaccharide,SFPS 191212,is 43 kDa.SFPS 191212 is composed of mannose,rhamnose,galactose,xylose,glucose,and fucose(at a molar ratio:2.1:2.9:1.8:15.5:4.6:62.5)withα-andβ-configurations.The present research evaluated the anti-tumor potential of the S.fusiforme polysaccharide in human erythroleukemia(HEL)cells in vitro.To explore the SFPS 191212’s apoptosis mechanism in HEL cells,transcriptome analysis was performed on HEL cells that were incubated with SFPS 191212.The inhibitory effect of SFPS 191212 on HEL cell growth was also analyzed.It was found that SFPS 191212 inhibited HEL cell proliferation,reduced cell viability in a concentration-dependent manner,and induced an insignificant toxic effect on normal human embryonic lung(MRC-5)cells.Compared with the control group,transcriptome analysis identified a total of 598 differentially expressed genes(DEGs),including 243 up-regulated genes and 355 downregulated genes.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analyses were performed on all DEGs,and 900 GO terms and 52 pathways were found to be significantly enriched.Finally,23 DEGs were randomly selected and confirmed by quantitative real-time polymerase chain reaction(qRT-PCR).Moreover,SFPS 191212 down-regulated the PI3K/Akt signal transduction pathway.Our results provide a framework for understanding the effect of SFPS 191212 on cancer cells and can serve as a resource for delineating the anti-tumor mechanisms of S.fusiforme.

关键词： Human erythroleukemia Polysaccharide sargassum fusiforme Transcriptome

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Effect of sargassum fusiforme polysaccharide on apoptosis and its possible mechanism in human erythroleukemia cells

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Chinese Journal of Natural Medicines 2020年第10期18卷 749-759页

作者： DING Hao-Miao CHEN Xue-Jia CHEN Hai-Min WANG Cai-Sheng QIAN Guo-Ying School of Marine Sciences Ningbo UniversityNingbo 315211China College of Biological and Environmental Sciences Zhejiang Wanli UniversityNingbo 315100China

This study aimed to investigate the effects of sargassum fusiforme polysaccharide(SFPS I,II,and III)on the apoptosis and regulation of human erythroleukemia(HEL)cells.The effect of different doses of SFPS on HEL cell growth was detected using the Cell Counting Kit-8 method,and apoptosis was detected by Hoechst staining.Cell cycle distribution and apoptosis were detected using flow cytometry.Expression of the cell cycle gene,p53,antiapoptotic genes,Bcl-xL and Bcl-2,and pro-apoptotic genes,Bax,Bad,and Caspase-3,as well as the expression of the corresponding proteins,were detected using real-time quantitative polymerase chain reaction(qPCR)and Western blot.The results showed that SFPS Ⅱ and Ⅲ decreased HEL cell viability and induced HEL cell apoptosis.Different concentrations of SFPS(Ⅰ,Ⅱ,and Ⅲ)were detected that induced much less toxic effect in normal human embryonic lung(MRC-5)cells,and SFPS Ⅰ increased cell proliferation,indicating its favorable selectivity towards cancer cells.The mechanism by which SFPS induced apoptosis was also found to be related to the induction of cell cycle arrest in the G0/G1 phase and the increased expression of apoptosis-related genes and proteins.We concluded that SFPS induces HEL cell apoptosis,possibly via activation of the Caspase pathway,providing the theoretical basis for the development of SFPS-based anti-tumor drug products.

关键词： sargassum fusiforme Polysaccharides Human erythroleukemia(HEL cells) Apoptosis Cell cycle

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Preliminary studies on the chemical characterization and antioxidant properties of acidic polysaccharides from sargassum fusiforme

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2008年第9期9卷 721-727页

作者： Jing ZHOU, Nan HU, Ya-lin WU, Yuan-jiang PAN, Cui-rong SUN (Department of Chemistry, Zhejiang University, Hangzhou 310027, China) Department of Chemistry Zhejiang University Hangzhou 310027 China.

In order to investigate the antioxidant properties of the polysaccharides from the brown alga sargassum fusiforme, the crude polysaccharides from S. fusiforme (SFPS) were extracted in hot water, and the lipid peroxidation inhibition assay exhibited that SFPS possessed a potential antioxidant activity. Hence, two purely polymeric fractions, SFPS-1 and SFPS-2 were isolated by the column of DEAE (2-diethylaminoethanol)-Sepharose Fast Flow, with their molecular weights of 51.4 and 30.3 kDa determined by high performance gel permeation chromatography (HPGPC). They were preliminarily characterized using chemical analysis in combination of infrared (IR) and nuclear magnetic resonance (NMR) spectroscopies and found to contain large amounts of uronic acids and β-glycosidical linkages. The antioxidant activities of these two SFPS fractions were evaluated using superoxide and hydroxyl radical-scavenging assays. The results show that the antioxidant ability of SFPS-2 was higher than that of SFPS-1, probably correlating with the molecular weight and uronic acid content.

关键词： sargassum fusiforme Polysaccharide Antioxidant activity Uronic acid Molecular weight

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Oxidative stress responses to cadmium in the seedlings of a commercial seaweed sargassum fusiforme

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Acta Oceanologica Sinica 2020年第10期39卷 147-154页

作者： Tiantian Zhang Minheng Hong Mingjiang Wu Binbin Chen Zengling Ma College of Life and Environmental Science Wenzhou UniversityWenzhou 325035China Zhejiang Provincial Key Laboratory for Subtropical Water Environment and Marine Biological Resources Protection Wenzhou 325035China

Cadmium(Cd)is a common heavy metal pollutant in the aquatic environment,generally toxic to plant growth and leading to growth inhibition and biomass reduction.To study the oxidation resistance in sargassum fusiforme seedlings in response to inorganic Cd stress,we cultured the seedlings under two different Cd levels:natural seawater and high Cd stress.High Cd stress significantly inhibited the seedlings growth,and darkened the thalli color.Additionally,the pigment contents,growth rate,peroxidase(POD)activity,dehydroascorbic acid(DHA)content,and glutathione reductase(GR)activity in S.fusiforme were significantly reduced by high Cd treatment.Contrarily,the Cd accumulation,Cd2+absorption rate,dark respiration/net photosynthetic rate(Rd/Pn),ascorbic acid(Vc)content,soluble protein(SP)content,glutathione(GSH),and the activities of superoxide dismutase(SOD)and catalase(CAT)of S.fusiforme under Cd treatment significantly increased compared to the control group.The decrease of malondialdehyde(MDA)was not significant.Although S.fusiforme seedlings increased the antioxidant activities of POD,SOD,Vc,and the AsA-GSH cycle to disseminate H2O2 and maintain healthy metabolism,high Cd stress caused Cd accumulation in the stem and leaves of S.fusiforme seedlings.The excessive Cd significantly restricted photosynthesis and reduced photosynthetic pigments in the seedlings,resulting in growth inhibition and deep morphological color,especially of the stems.High levels of Cd in seawater had toxic effects on commercial S.fusiforme seedlings,and risked this edible seaweed for human food.

关键词： sargassum fusiforme cadmium stress oxidation resistance photosynthesis aquaculture marine pollution

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Use of sargassum fusiforme Extract and its Bioactive Molecules to inhibit HIV Infection: Bridging Two Paradigms between Eastern and Western Medicine

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Chinese Herbal Medicines 2014年第4期6卷 265-273页

作者： Hui-jun Guo Yan-ze Liu Elena E.Paskaleva Manoj Arra Jeffrey S.Kennedy Alexander Shekhtman Mario Canki First Affiliated Hospital of Henan University of Traditional Chinese Medicine Institute of Medicinal Plant Development Chinese Academy of Medical Sciences & Peking Union Medical College Palm Biologicals LLC.AlbanyUSA Center for Biotechnology & Interdisciplinary Studies Rensselaer Polytechnic InstituteTroyUSA Center for Immunology and Microbial Disease Albany Medical CollegeAlbanyUSA Translational Medicine Albany Medical CollegeAlbanyUSA Department of Chemistry State University of New York at AlbanyAlbanyUSA

AIDS is a global pandemic that has seen the development of novel and effective treatments to improve the quality of life of those infected and reduction in the spread of the disease. While great advancements have been made in HIV/AIDS therapeutics, there is still no cure or viable vaccine in development. The high rate of HIV-1 mutation contributing to virus immune escape, combined with increase in sexual transmission and the significant clinical therapeutics side effects of the currently available treatments highlights the need for novel therapeutics with broad anti-viral activity against both CXCR4 （X4） and CCR5 （R5）-tropic viruses. In our search for novel modalities against HIV infection, we investigated several aqueous extracts from Traditional Chinese Medicine （TCM） collection and identified sargassum fusiforme （S. fusiforme） as a potent inhibitor of HIV infection. Following the Western approach of drug discovery and development, we isolated several bioactive molecules from S. fusiforme and determined their mechanism of action. TCM and Western approaches to disease treatment and drug development have been shown to be complementary to each other. The former provides a rich medicinal history for natural compounds that can have clinical impact on a variety of illnesses, while the latter enables the application of chemical informatics with rational drug design approach coupled to mechanistic underpinnings of such therapeutics. This multistep paradigm is demonstrated in the example of S. fusiforme as a treatment to prevent HIV infection, as described in this review.

关键词： AIDS anti-infection chemical entities sargassum fusiforme

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The effects of polyphenols from sargassum fusiforme on the conformational structure and allergenicity of shrimp tropomyosin

The effects of polyphenols from Sargassum fusiforme on the c...

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中国营养学会第十五届全国营养科学大会

作者： Ziye Zhang Yiming Zhao Zhenxing Li Hong Lin Laboratory of Food Safety College of Food Science and Engineering Ocean University of China

Shrimp allergy is a common foodborne disease that could trigger severe allergic reactions and usually persist throughout life. sargassum fusiforme is an edible brown alga(seaweed) that harvested on the Pacific coast of China, this work investigates the effects of polyphenols from sargassum fusiforme on the conformational structure and allergenicity of shrimp tropomyosin using UV spectra, intrinsic fluorescence, surface hydrophobicity, FT-IR, indirect ELISA and RBL-2 H3 mast cell. As the results, the conjugation of sargassum fusiforme polyphenols to TM induced the instability of TM conformational structures, led to lower IgE/IgG-binding capacity of TM, and weakened the ability of TM to stimulate the allergic reactions of mast cells. In conclusion, sargassum fusiforme polyphenols could perform antiallergic functions and serve as candidate natural anti-allergic substances to alleviate food allergy. This work could provide a new insight for sargassum fusiforme polyphenols into hypoallergenic foods, which could be beneficial for food allergy prevention and treatment. Methods In this work, the polyphenol monomers from sargassum fusiforme were extracted and purified using macroporous resin and preparative high performance liquid chromatography(HPLC), the conformational structure was analyzed by liquid chromatography mass spectrometry(LC-MS), the conjugation degree of sargassum fusiforme polyphenol to TM was analyzed using SDS-PAGE, the conformational structural changes were evaluated using UV spectra, intrinsic fluorescence, surface hydrophobicity and FT-IR data, the IgE/IgG-binding capacity of TM were determined using indirect ELISA, and the allergenicity of TM was investigated using RBL-2 H3 mast cell. Results After purification by preparative HPLC, the conformational structure of sargassum fusiforme polyphenol was determined using HPLC-MS. As the MS-MS spectra, the ion 1 with [M+H]+ at m/z 321.17 corresponded to the quercetin monohydrate, while fragment MS2 fragment-303.16 corresponded to the loss of one water molecu

关键词： sargassum fusiforme Polyphenols Shrimp Tropomyosin Allergenicity

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Antidepressant-like effects of saringosterol, a sterol from sargassum fusiforme by performing in vivo behavioral tests

Antidepressant-like effects of saringosterol, a sterol from ...

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2018年浙江省神经病学学术年会

作者： Hong-Guo Jin Min Zhou Qing-Hao Jin Bing-Yu Liu Li-Ping Guan Department of Neurology Zhoushan Hospital of Zhejiang Province Food and Pharmacy College Zhejiang Ocean University and Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products

Herein, two classic animal behavior despair teststhe forced swim test and tail suspension test were used to evaluate the antidepressant-like activity of saringosterol from sargassum fusiforme in mice. Saringosterol was found to significantly shorten immobility time in the forced swim test and tail suspension test at doses of 10, 20, and 30 mg/kg in mice. The measurement of locomotor activity indicated that saringosterol had no central nervous systemstimulating effects. In addition, it was found that the saringosterol significantly increased noradrenaline, serotonin(5-HT), and the metabolite 5-hydroxyindoleacetic acid in the mouse brain, suggesting that the antidepressant-like activity may be mediated through these neurotransmitters.

关键词： sargassum fusiforme Sterol Saringosterol Antidepressant effect

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Studies on antivirus effects of polysacoharides from sargassum fusiforme in vitro

Studies on antivirus effects of polysacoharides from Sargass...

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中国海洋生化学术会议

作者： CEN Ying-zhou, KHOO Gaik-ming, WANG Ling-yun, XU Shao-yu, ZHANG Mei-ying, WANG Yi-fei Department of Chemistry, Faculty of Life Science and Technology, Jinan University, Guangzhou, Guangdong, China 510632 Foshan Agrotechnical Station, Guangdong, China 528000 Guangzhou Jinan Biomedical Research and Development Center, Jinan University, Guangzhou, Guangdong, China 510632

Objective: To assay antiviral effects of the polysaccharide and its separated products from sargassum fusiforme on HSV-1 and CVB3 and their antiviral mechanism. Method: The antiviral effects of all samples on HSV-1 and CVB3 and their cytotoxicity on Vero cells were studied by CPE and MTT method. Antiviral mechanism of two samples was briefly studied by four different ways. Results: (?) The cytotoxicity of samples on vero cells was not detected (CC>5000μg/mL). (?) All samples except PSJ had obviously HSV-1 inhibitory effect. Antiviral activities of them were increasingly strengthened with raising their purities. Antiviral activities of F1, F2 and F4 were better than ACV. ∈Antiviral activities of all samples on CVB3 were better than ribavirin injection. F1, F2 and F4 had remarkable anti-CVB3. (?) The experiment showed that polysaccharide not only able to kill the above viruses directly but also restrain them by getting into cell or absorbing on cells.

关键词： sargassum fusiforme polysaccharide HSV-1 CVB3 antiviral

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Antidepressant-like effects of saringosterol,a sterol from sargassum fusiforme by performing in vivo behavioral tests

Antidepressant-like effects of saringosterol,a sterol from S...

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2018年浙江省老年精神障碍学术年会

Herein,two classic animal behavior despair tests-the forced swim test and tail suspension test were used to evaluate the antidepressant-like activity of saringosterol from sargassum fusiforme in mice.Saringosterol was found to significantly shorten immobility time in the forced swim test and tail suspension test at doses of 10,20,and 30 mg/kg in mice.The measurement of locomotor activity indicated that saringosterol had no central nervous systemstimulating effects.In addition,it was found that the saringosterol significantly increased noradrenaline,serotonin(5-HT),and the metabolite 5-hydroxyindoleacetic acid in the mouse brain,suggesting that the antidepressant-like activity may be mediated through these neurotransmitters.

关键词： sargassum fusiforme Sterol Saringosterol Antidepressant effect

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Adsorption of congo red on mesoporous activated carbon prepared by CO2 physical activation

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Chinese Journal of Chemical Engineering 2020年第4期28卷 1069-1076页

作者： Mingjie Ma Huijuan Ying Fangfang Cao Qining Wang Ning Ai College of Chemical Engineering Zhejiang University of TechnologyHangzhou 310014China Zhejiang Province Key Laboratory of Biomass Fuel Hangzhou 310014China Biodiesel Laboratory of China Petroleum and Chemical Industry Federation Hangzhou 310014China National Chemical and Chemical Engineer Experiment Teaching Center National Chemical and Chemical Engineer Simulation Experiment Teaching CenterZhejiang University of TechnologyHangzhou 310014China

This research demonstrates the production of mesoporous activated carbon from sargassum fusiforme via physical activation with carbon dioxide.Central composite design was applied to conduct the experiments at different levels by altering three operating parameters.Activation temperature(766-934℃),CO2 flow rate(0.8-2.8 L·min^-1)and activation time(5-55 min)were the variables examined in this study.The effect of parameters on the specific surface area,total pore volume and burn-out rate of activated carbon was studied,and the influential parameters of methylene blue adsorption value were identified employing analysis of variance.The optimum conditions for maximum methylene blue adsorption value were:activation temperature=900℃,activation time=29.05 min and CO2 flow rate=1.8 L·min(-1).The activated carbon produced under optimum conditions was characterized by BET,FTIR and SEM.The adsorption behavior on congo red was studied.The effect of parameters on the adsorbent dosage,temperature,PH and initial congo red concentration was investigated.The adsorption properties of the activated carbon were investigated by kinetics.The equilibrium removal rate and maximum adsorption capacity reaches up to 94.72%,234 mg·g^-1,respectively when initial congo red concentration is 200 mg·L^-1 under adsorbent dosage(0.8 g·L^-1),temperature(30℃),PH7.

关键词： sargassum fusiforme Mesoporous activated carbon Congo red Response surface method Kinetics

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