Transcriptomic profile of human erythroleukemia cells in response to Sargassum fusiforme polysaccharide and its structure analysis

作     者：DING Hao-Miao FU Rui-Jie XIE Ce WANG Cai-Sheng QIAN Guo-Ying DING Hao-Miao;FU Rui-Jie;XIE Ce;WANG Cai-Sheng;QIAN Guo-Ying

作者机构：College of Biological and Environmental SciencesZhejiang Wanli UniversityNingbo 315100China School of Marine SciencesNingbo UniversityNingbo 315211China 

出 版 物：《Chinese Journal of Natural Medicines》 (中国天然药物（英文版）)

年 卷 期：2021年第19卷第10期

页      面：784-795页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：partially funded by Zhejiang Wanli University Scientific Research and Innivation Team(No.SC1032110880210) Zhejiang Provincial Top Discipline of Biological Engineering(No.KF2021010) Ningbo Public Service Platform for High-Value Utilization of Marine Biological Resources(Nos.NBHY-2017-S5,NBHY-2017(1)) 

主　　题：Human erythroleukemia Polysaccharide Sargassum fusiforme Transcriptome 

摘      要：Sargassum fusiforme(***)has been used as an ingredient in Chinese herbal medicine for thousands of ***,there are a limited number of studies concerning its therapeutic *** performance gel permeation chromatography(HPGPC)analysis showed that the average molecular weight of the *** polysaccharide,SFPS 191212,is 43 *** 191212 is composed of mannose,rhamnose,galactose,xylose,glucose,and fucose(at a molar ratio:2.1:2.9:1.8:15.5:4.6:62.5)withα-andβ-*** present research evaluated the anti-tumor potential of the *** polysaccharide in human erythroleukemia(HEL)cells in *** explore the SFPS 191212’s apoptosis mechanism in HEL cells,transcriptome analysis was performed on HEL cells that were incubated with SFPS *** inhibitory effect of SFPS 191212 on HEL cell growth was also *** was found that SFPS 191212 inhibited HEL cell proliferation,reduced cell viability in a concentration-dependent manner,and induced an insignificant toxic effect on normal human embryonic lung(MRC-5)*** with the control group,transcriptome analysis identified a total of 598 differentially expressed genes(DEGs),including 243 up-regulated genes and 355 downregulated *** Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analyses were performed on all DEGs,and 900 GO terms and 52 pathways were found to be significantly ***,23 DEGs were randomly selected and confirmed by quantitative real-time polymerase chain reaction(qRT-PCR).Moreover,SFPS 191212 down-regulated the PI3K/Akt signal transduction *** results provide a framework for understanding the effect of SFPS 191212 on cancer cells and can serve as a resource for delineating the anti-tumor mechanisms of ***.