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Morphotaxonomic and phylogenetic analysis of saprolegnia ferax from India

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Studies in Fungi 2018年第1期3卷 49-58页

作者： Singh PN Singh SK Lagashetti AC Ackah FK National Fungal Culture Collection of India Biodiversity and Palaeobiology GroupMACS’Agharkar Research InstituteGG Agarkar RoadPune 411004India Department of Crop Science School of AgricultureCollege of Agriculture and Natural SciencesUniversity of Cape CoastGhana

saprolegnia ferax is isolated from polluted water sample collected from Mula River,Pune,Maharashtra,*** isolated taxon is illustrated and compared with the morphotaxonomy based first record of Indian *** isolated by Wani et al.(2017).The present study deals with the re-description of the isolated taxon *** based on detail morphological features,sequence analysis and phylogeny of ITS and LSU regions of rDNA.

关键词： ITS and LSU rDNA Morphotaxonomy Oomycete saprolegnia

Inhibitory effects of silver zeolite on in vitro growth of fish egg pathogen,saprolegnia sp.

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Journal of Coastal Life Medicine 2014年第5期 357-361页

作者： Seyed Ali Johari Mohammad Reza Kalbassi Il Je Yu Fisheries Department Natural Resources FacultyUniversity of KurdistanSanandajIran Department of Aquaculture Marine Science FacultyTarbiat Modares UniversityMazandaranNoorIran Institute of Nanoproduct Safety Research Hoseo University165 Sechul-riBaebang-myunAsan 336-795Korea

Objective:To investigate the effects of powdered silver zeolite(SZ)on the in vitro growth of the fish pathogen saprolegnia ***:The antifungal activity of SZ was evaluated by determining the minimum inhibitory concentrations using two-fold serial dilutions of powdered SZ in a glucose yeast extract agar at 22℃.The growth of saprolegnia *** the SZ agar treatments was compared to that on SZ-free agar ***:The results showed that SZ had an inhibitory effect on the in vitro growth of the tested *** minimum inhibitory concentration of SZ for saprolegnia *** also calculated at 600 mg/L,which is equal to 0.06 ***:SZ is a potential good candidate to replace teratogenic and toxic agents,such as malachite green in aquaculture systems.

关键词： Antifungal In vitro saprolegnia Silver zeolite Aquaculture

Identification and characterization of microbial agents in a bloom of floating microorganisms in the Hailang River,China in 2006

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Chinese Journal of Oceanology and Limnology 2010年第4期28卷 836-841页

作者：吴伟立王景林刘利成姜君陈燕胡涛陈唯军 Beijing Institute of Genomics Chinese Academy of SciencesBeijing Shunyi Airport Industrial Zone State Key Laboratory of Pathogen and Biosecurity Institute of Microbiology and EpidemiologyAcademy of Military Medical Sciences

In February 2006,a large amount of unknown floating microorganisms appeared in the Hailang River(HR) in the City of Hailin,Heilongjiang Province,*** microorganisms caused the river water fouling and threatened the clean water supply of the *** identify the unknown floating organisms,morphological and histological inspection,PCR,cloning and sequence analysis were *** results revealed that the unknown floating organisms in the river were a novel species that likely belonged to oomycetes in *** organisms were named saprolegnia ***0602.

关键词： unknown floating organisms Hailang River PCR sequence analysis saprolegnia

Molecular identification of oomycete species affecting aquaculture in Bangladesh

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Aquaculture and Fisheries 2019年第3期4卷 105-113页

作者： Mohammad Nasif Sarowar Md Jakir Hossain Tahmina Nasrin Tania Naznin Zakir Hossain Mohammad Matiur Rahman Department of Fisheries Biology and Genetics Bangladesh Agricultural UniversityMymensingh-2202Bangladesh Department of Animal Science and Aquaculture Faculty of AgricultureDalhousie UniversityAgriculture campusTruroNova Scotia B2N 5E3Canada

Fish mycotic disease outbreaks occur due to infections with oomycete pathogens such as saprolegnia *** Aphanomyces invadans,and cause large-scale fish production *** its negative impact on aquaculture,little is known about the diversity of oomycete *** aim of this study was to identify the diversity of pathogenic oomycetes causing infections in major aquaculture zones of Mymensingh and Jessore regions in Bangladesh.A total of 449 water and infected fish samples were collected from 28 fish farms in both regions of which 29 samples were able to grow out with mycelia on the Potato Dextrose Agar(PDA)/Glucose Peptone Yeast Agar(GPYA)*** database searches using the rRNA Internal Transcribed Spacer(ITS)region revealed that 15 belonged to Pythium spp.,12 were Aphanomyces invadans and two corresponded to saprolegnia *** isolates of Pythium *** identified to the species level:one was closely related to Pythium catenulatum,four to Pythium rhizo-oryzae,rest were identified up to *** Pythium *** only isolated from water samples whereas *** and *** were found in fish *** analysis revealed that a single *** clone exists in the sampled *** results obtained confirm the existence of pathogenic oomycetes in Bangladesh fish farms and this will pave future research on diversity,prevention and control measures.

关键词： Oomycete saprolegnia Aphanomyces invadans Internal transcribed spacer(ITS) Phylogeny