Molecular identification of oomycete species affecting aquaculture in Bangladesh

作     者：Mohammad Nasif Sarowar Md Jakir Hossain Tahmina Nasrin Tania Naznin Zakir Hossain Mohammad Matiur Rahman 

作者机构：Department of Fisheries Biology and GeneticsBangladesh Agricultural UniversityMymensingh-2202Bangladesh Department of Animal Science and AquacultureFaculty of AgricultureDalhousie UniversityAgriculture campusTruroNova Scotia B2N 5E3Canada 

出 版 物：《Aquaculture and Fisheries》 (渔业学报（英文）)

年 卷 期：2019年第4卷第3期

页      面：105-113页

学科分类：09[农学] 0904[农学-植物保护] 

基　　金：The authors would like to express deepest sense of gratitude and sincere appreciation for the financial support of the International Foundation for Science,Sweden(IFS:A/5788-1) COMSTECH as well as the Ministry of Science and Technology,Bangladesh(BS-57,2015–2016) TN acknowledges NST fellowship Ministry of Science and Technology,Bangladesh.The authors would also like to acknowledge the contribution of the Head of the department,Department of Fisheries Biology and Genetics,and Central Laboratory,Bangladesh Agricultural University for providing equipment and technical supports during the research 

主　　题：Oomycete Saprolegnia Aphanomyces invadans Internal transcribed spacer(ITS) Phylogeny 

摘      要：Fish mycotic disease outbreaks occur due to infections with oomycete pathogens such as Saprolegnia *** Aphanomyces invadans,and cause large-scale fish production *** its negative impact on aquaculture,little is known about the diversity of oomycete *** aim of this study was to identify the diversity of pathogenic oomycetes causing infections in major aquaculture zones of Mymensingh and Jessore regions in Bangladesh.A total of 449 water and infected fish samples were collected from 28 fish farms in both regions of which 29 samples were able to grow out with mycelia on the Potato Dextrose Agar(PDA)/Glucose Peptone Yeast Agar(GPYA)*** database searches using the rRNA Internal Transcribed Spacer(ITS)region revealed that 15 belonged to Pythium spp.,12 were Aphanomyces invadans and two corresponded to Saprolegnia *** isolates of Pythium *** identified to the species level:one was closely related to Pythium catenulatum,four to Pythium rhizo-oryzae,rest were identified up to *** Pythium *** only isolated from water samples whereas *** and *** were found in fish *** analysis revealed that a single *** clone exists in the sampled *** results obtained confirm the existence of pathogenic oomycetes in Bangladesh fish farms and this will pave future research on diversity,prevention and control measures.