检索结果-南通市图书馆

salvianolic Acid A Protects Neonatal Cardiomyocytes against Hypoxia/Reoxygenation-Induced Injury by Preserving Mitochondrial Function and Activating Akt/GSK-3β Signals

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Integrative Medicine 2019年第1期25卷 23-30页

作者： LI Xue-li FAN Ji-ping LIU Jian-xun LIANG Li-na Institute of Basic Medical Sciences Xiyuan Hospital China Academy of Chinese Medical Sciences Experimental Research Center China Academy of Chinese Medical Sciences Post-doctoral Research Station China Academy of Chinese Medical Sciences China Academy of Chinese Medical Sciences Eye Function Laboratory Yanke Hospital China Academy of Chinese Medical Sciences

Objective: To investigate the effects of salvianolic acid A(SAA) on cardiomyocyte apoptosis and mitochondrial dysfunction in response to hypoxia/reoxygenation(H/R) injury and to determine whether the Akt signaling pathway might play a role. Methods: An in vitro model of H/R injury was used to study outcomes on primary cultured neonatal rat cardiomyocytes. The cardiomyocytes were treated with 12.5, 25, 50 μg/m L SAA at the beginning of hypoxia and reoxygenation, respectively. Adenosine triphospate(ATP) and reactive oxygen species(ROS) levels were assayed. Cell apoptosis was evaluated by flow cytometry and the expression of cleavedcaspase 3, Bax and Bcl-2 were detected by Western blotting. The effects of SAA on mitochondrial dysfunction were examined by determining the mitochondrial membrane potential(△Ψm) and mitochondrial permeability transition pore(m PTP), followed by the phosphorylation of Akt(p-Akt) and GSK-3β(p-GSK-3β), which were measured by Western blotting. Results: SAA significantly preserved ATP levels and reduced ROS production. Importantly, SAA markedly reduced the number of apoptotic cel s and decreased cleaved-caspase 3 expression levels, while also reducing the ratio of Bax/Bcl-2. Furthermore, SAA prevented the loss of △Ψm and inhibited the activation of m PTP. Western blotting experiments further revealed that SAA significantly increased the expression of p-Akt and p-GSK-3β, and the increase in p-GSK-3β expression was attenuated after inhibition of the Akt signaling pathway with LY294002. Conclusion: SAA has a protective effect on cardiomyocyte H/R injury; the underlying mechanism may be related to the preservation of mitochondrial function and the activation of the Akt/GSK-3β signaling pathway.

关键词： salvianolic acid A cardiomyocyte hypoxia/reoxygenation injury mitochondria Akt/GSK-3β

Inhibition of FOXO3a/BIM signaling pathway contributes to the protective effect of salvianolic acid A against cerebral ischemia/reperfusion injury

在线全文

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2019年第3期9卷 505-515页

作者： Junke Song Wen Zhang Jinhua Wang Haiguang Yang Qimeng Zhou Haigang Wang Li Li Guanhua Du State Key Laboratory of Bioactive Substances and Functions of Natural Medicines Institute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing100050China Beijing Key Laboratory of Drug Target Identification and Drug Screening Institute of Materia MedicaChinese Academy of MedicalSciences and Peking Union Medical CollegeBeijing100050China

salvianolic acid A(SalA) is an effective compound extracted from traditional Chinese medicine Salvia miltiorrhiza Bunge. The Forkhead box O3a(FOXO3a) signaling pathway plays crucial roles in the modulation of ischemia-induced cell apoptosis. However, no information about the regulatory effect of SalA on FoxO3a is available. To explore the anti-cerebral ischemia effect and clarify the therapeutic mechanism of SalA, SH-SY5Y cells and Sprague–Dawley rats were applied, which were exposed to oxygen glucose deprivation/reoxygenation(OGD/R) and middle cerebral artery occlusion/reperfusion(MCAO/R) injuries, respectively. The involved pathway was identified using the specific inhibitor LY294002. Results showed that SalA concentration-dependently inhibited OGD/R injury triggered cell viability loss. SalA reduced cerebral infarction, lowered brain edema, improved neurological function, and inhibited neuron apoptosis in MCAO/R rats, which were attenuated by the treatment of phosphatidylinositol-4,5-bisphosphate 3-kinase(PI3K) specific inhibitor LY294002. SalA time-and concentration-dependently upregulated the phosphorylation levels of protein kinase B(AKT) and its downstream protein FOXO3a. Moreover, the nuclear translocation of FOXO3a was inhibited by SalA both in vivo and in vitro, which was also reversed by LY294002. The above results indicated that SalA fought against ischemia/reperfusion damage at least partially via the AKT/FOXO3a/BIM pathway.

关键词： salvianolic acid A Ischemia reperfusion FOXO3a BIM Neuroprotection

SYNTHESIS　OF　TETRAMETHYL　salvianolic　ACID　F　AND　TRIMETHYL　PRZEWALSKINIC　ACID　A

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 1996年第5期7卷 427-430页

作者： Al, CB Li, LN CHINESE ACAD MED SCI INST MAT MEDBEIJING 100050PEOPLES R CHINA

Abstract: salvianolic acid F (1a) and przewalskinic add A(2a) are phenolic acids isolated from the traditional and herbal Chinese medicines Salvia miltiorrhiza (1) and S. przewalskii (2). The chemical structures of these two compounds represent the main carbon skeletons of the biologically active depsides salvianolicacid A and B isolated from S. miltionhiza (3.4). The present paper reports the synthesis of the methyl ether protected phenolic acids. tetramethyl salvianolic acid F(1b) and trimethyl prsewalskinic acid A(2b).

关键词： ACID TRIMETHYL TETRAMETHYL PRZEWALSKINIC salvianolic SYNTHESIS AND OF

Protective actions of salvianolic acid A on hepatocyte injured by peroxidation in vitro

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2000年第3期6卷 402-404页

作者： Hu YY Liu CH Wang RP Liu C Liu P Zhu DY Shanghai Univ Tradit Chinese Med Inst Liver Dis Shanghai 200032 Peoples R China

INTRODUCTION salvianolic radix,one of the most commonly usedtraditional Chinese herbs,was widely studied aboutits actions against liver injury and fibrosis,and wasone of the focuses of recent *** acid-A(SA-A)was an aq... 详细信息

关键词： salvianolic acid A hepatocyte carbon tetrachloride liver injury lipid peroxidation water soluble vitamin E ALT AST superoxide dismutase malondiadehyde catalase lactase dehydrogenase glutathione peroxidase glutathione

Effects of salvianolic acid B on liver mitochondria of rats with nonalcoholic steatohepatitis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2015年第35期21卷 10104-10112页

作者： Ying-Chun Wang Wei-Zong Kong Qing-Mei Jin Juan Chen Lei Dong Department of Gastroenterology the Second Affiliated HospitalXi’an Jiaotong University Department of Gastroenterology Affiliated Zhongshan Hospital of Dalian University

AIM: To investigate the effects of salvianolic acid B(Sal B) on the morphological characteristics and functions of liver mitochondria of rats with nonalcoholic steatohepatitis(NASH).METHODS: A total of 60 male Sprague-Dawley rats were randomly divided into three groups:(1) a normal group fed a normal diet;(2) an NASH model group; and(3) a Sal B-treated group fed a high-fat diet. Two rats from each group were executed at the end of the 12 th week to detect pathological changes. The rats in the Sal B-treated group were gavaged with 20 m L/kg Sal B(1 mg/m L) daily. The model group received an equal volume of distilled water as a control. At the end of the 24 th weekend, the remaining rats were executed. Serum biochemical parameters and liver histological characteristics were observed. Malondialdehyde(MDA) and superoxide dismutase(SOD) in the liver were determined. Protein expression of Cyt C and caspase-3 was determined by immunohistochemistry. The m RNA transcripts of mitofusin-2(Mfn2) and NF-κB in the liver tissue were detected by real-time PCR. Mitochondrial membrane potential was detected using a fluorescence spectrophotometer. Mitochondrial respiratory function was detected using a Clark oxygen ***: The model group showed significantly higher ALT, AST, TG, TC and MDA but significantly lower SOD than the normal group. In the model group,the histological characteristics of inflammation and steatosis were also evident; mitochondrial swelling and crest were shortened or even disappeared. Cyt C(18.46 ± 1.21 vs 60.01 ± 3.43, P < 0.01) and caspase-3 protein expression(30.26 ± 2.56 vs 83.31 ± 5.12, P < 0.01) increased significantly. The m RNA expression of NF-κB increased(0.81 ± 0.02 vs 0.91 ± 0.03, P < 0.05), whereas the m RNA expression of Mfn2 decreased(1.65 ± 0.31 vs 0.83 ± 0.16, P < 0.05). Mitochondrial membrane potential also decreased and breathing of rats was weakened. Steatosis and inflammation degrees in the treatment group were significantly allev

关键词： Nonalcoholic steatohepatitis salvianolic acid B Ap

Effects of salvianolic acid B on proliferation, neurite outgrowth and differentiation of neural stem cells derived from the cerebral cortex of embryonic mice

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2010年第6期53卷 653-662页

作者： GUO GuoQing1,LI Bin1,WANG YuanYuan1,SHAN AiJing1,SHEN WeiZai1,YUAN Lin2 & ZHONG ShiZhen2 1Department of Anatomy,Medical College of Jinan University,Guangzhou 510630,China 2Institute of Clinical Anatomy,Key Laboratory of Tissue Construction and Detection of Guangdong Province,Southern Medical University,Guangzhou 510150,China 1. Department of Anatomy Medical College of Jinan University Guangzhou 510630 China2. Institute of Clinical Anatomy Key Laboratory of Tissue Construction and Detection of Guangdong Province Southern Medical University Guangzhou 510150 China

salvianolic acid B is isolated from Salvia miltiorrhiza,the root of which is widely used as a traditional Chinese medicine to treat ***,little is known about how salvianolic acid B influences growth characteristics of neural stem cells (NSCs).The purpose of the present study was to evaluate the effects of salvianolic acid B on proliferation,neurite outgrowth and differentiation of NSCs derived from the cerebral cortex of embryonic mice using MTT,flow cytometry,immunofluorescence and *** was found that 20 μg mL·1 and 40 μg mL·1 salvianolic acid B had similar effects on proliferation of NSCs,and a suitable concentration of salvianolic acid B increased the number of NSCs and their derivative *** growth-promoting activity of salvianolic acid B was dependent on and associated with an accumulation in the G2/S-phase cell *** acid B also promoted the neurite outgrowth of NSCs and their differentiation into *** mRNA for tau,GFAP and nestin were present in differentiating neurospheres induced by salvianolic acid ***,high-level expression of tau mRNA and low-level expression of GFAP mRNA was detected in differentiated cells,in contrast to the control *** collective evidence indicates that exogenous salvianolic acid B is capable of promoting proliferation of neurospheres and differentiation towards the neuronal lineage in vitro and may act in the proliferation of NSCs and may promote NSC differentiation into neuronal cells.

关键词： salvianolic acid B proliferation differentiation neurite outgrowth neural stem cells cerebral cortex embryonic mouse

Effect and mechanism of salvianolic acid B on the myocardial ischemiareperfusion injury in rats

在线全文

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Medicine 2014年第4期7卷 280-284页

作者： Ling Xue Zhen Wu Xiao-Ping Ji Xia-Qing Gao Yan-Hua Guo Department of Cardiovascular Medicine Qilu HospitalShandong University Department of Cardiovascular Medicine Third Affiliated Hospital of Liaoning Medical College

Objective:To investigate the effect of salvianolic acid B on rats with myocardial ischemiareperfusion ***:SD rats were randomly divided into five groups(n=10 in each group):A sham operation group,B ischemic reperfusion group model group,C low dose salvianolic acid B group,D median dose salvianolic acid B group,E high dose salvianolic acid B *** hour after establishment of the myocardial ischemia-reperfusion model,the concentration and the apoptotic index of the plasma level of myocardial enzymes(CTnⅠ,CKMB),SOD,MDA,NO,ET were,*** tissues were obtained and micro-structural changes were ***:Compared the model group,the plasma CTnⅠ,CK-MB,MDA and ET contents were significantly increased,NO,T-SOD contents were decreased in the treatment group(group C,D,and E)(Psalvianolic acid B has a significant protective effect on myocardial ischemia-reperfusion *** can alleviate oxidative stress,reduce calcium overload,improve endothelial function and so on.

关键词： Ischemia-reperfusion injury Myocardial infarction salvianolic acid B Inflammatory response

Protective　effects　of　salvianolic　acid　A　against　impairment　of　memory　induced　by　cerebral　ischemia-reperfusion　in　mice

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Medical Journal 1997年第1期 63-66页

作者：杜冠华张均田

ＰｒｏｔｅｃｔｉｖｅｅｆｅｃｔｓｏｆｓａｌｖｉａｎｏｌｉｃａｃｉｄＡａｇａｉｎｓｔｉｍｐａｉｒｍｅｎｔｏｆｍｅｍｏｒｙｉｎｄｕｃｅｄｂｙｃｅｒｅｂｒａｌｉｓｃｈｅｍｉａｒｅｐｅｒｆｕｓｉｏｎｉｎｍｉｃｅＤｕＧｕａｎ... 详细信息

ＰｒｏｔｅｃｔｉｖｅｅｆｅｃｔｓｏｆｓａｌｖｉａｎｏｌｉｃａｃｉｄＡａｇａｉｎｓｔｉｍｐａｉｒｍｅｎｔｏｆｍｅｍｏｒｙｉｎｄｕｃｅｄｂｙｃｅｒｅｂｒａｌｉｓｃｈｅｍｉａｒｅｐｅｒｆｕｓｉｏｎｉｎｍｉｃｅＤｕＧｕａｎｈｕａ杜冠华ａｎｄＺｈａｎｇＪｕｎ...

关键词： salvianolic effects Protective acid

salvianolic acid B improves glucolipid metabolism by regulating adipogenic transcription factors in mice with diet-induced obesity

在线全文

学校读者我要写书评

暂无评论

Journal of Traditional Chinese Medical Sciences 2017年第3期4卷 280-289页

作者： Dandan Zhao Jiacheng Zuo Na Yu Xin Fang Fangfang Mo Rui Wu Tian Tian Rufeng Ma Yushan Gao Dongwei Zhang Guangjian Jiang Sihua Gao Beijing University of Chinese Medicine Beijing 100029China Department of Endocrinology Beijing University of Chinese MedicineThird Affiliated HospitalBeijing 100029China Department of Endocrinology South Area of Guang'anmen HospitalChina Academy of Chinese Medical SciencesBeijing 102618China

Objective:To determine the effect of salvianolic acid B (Sal B) on glucose and lipid metabolism in mice with high-fat diet (HFD)-induced obesity,and to investigate the underlying mechanisms by measuring the expression levels of key adipogenic transcription ***:Six-week-old C57BL/6J male mice were fed for 12 weeks with a HFD to induce obesity or a standard diet to serve as normal controls.A mean body weight increase of more than 20% after these 12 weeks was used as the criteria for ***-fed obese mice then received a supplement of Sal B (100 mg/kg body weight/day),metformin (75 mg/kg body weight/day) or water (an equivalent volume;served as model controls) by oral gavage for an additional 8 weeks,and the normal controls received water (an equivalent volume) by oral gavage for the same ***:Sal B significantly reduced body weight gain (P <.05) without influencing food intake in HFD-fed obese mice relative to model *** B also reduced the body fat mass of the obese mice relative to model controls in a time-dependent manner (P <.05).Sal B significantly decreased the serum concentrations of low-density lipoprotein cholesterol,total cholesterol,triglyceride and free fatty acids by 25.5%,20.2%,20.6% and 13.4%,respectively,and increased the concentration of high-density lipoprotein cholesterol by 50.1% relative to model *** addition,Sal B significantly lowered fasting glucose concentrations and improved insulin sensitivity relative to model controls (P <.05).Sal B acted by ameliorating the histopathological changes in both brown and white adipose tissues of obese ***,in brown adipose tissue,Sal B up-regulated the mRNA and protein expression of PPARγ and c/EBPα,and the protein expression of PPARα and SREBP-1 (P <.05).In white adipose tissue,Sal B down-regulated the mRNA expression of PPARγ and c/EBPα,and decreased the protein expression of PPARγ and SREBP-1(P <.05).Conclusjons:The results suggest that Sal B can reduce bo

关键词： salvianolic acid B Obesity Blood glucose Adipose tissue Adipogenic transcription factors