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High Efficiency of Propagation for sagittaria sagittifolia Using a Temporary Immersion Bioreactor System

Journal of Agricultural Science and Technology(A) 2016年第3期6卷 161-170页

作者： Meiping Gao Zhicheng Lin Cong Luo Zhang Chi Zhihuan Mo Fanglian He Wen Jiang Lijuan Chen Xinhua He Shaolong Wei Biotechnology Research Institute Guangxi Academy of Agricultural Sciences No. 174 Daxue Road Nanning Guangxi 530007 China College of Agriculture Guangxi University No. 100 Daxue Road Nanning Guangxi 530007 China Agricultural Bureau in Ping Le Areas Guangxi 542400 China

Although several studies have reported on the propagation of the sagittaria sagittifolia to date, none of these methods have efficiently achieved the mass production of these plants. The present study aimed to investigate the propagation and growth of S. sagittifolia using a temporary immersion bioreactor system （TIBS） compared with conventional semi-solid and liquid culture. The effect of different immersion frequencies and immersion times together with supplementation of various plant growth regulators to the Murashige and Skoog （MS） medium was evaluated on shoot proliferation and plant growth. The results showed that the higher immersion frequency （every 6 h） and shorter immersion time （3 min and 10 rain） in medium containing 4 mg/L BA and 0.1 mg/L NAA produced the highest multiplication rate （23）, which are significantly higher than conventional semi-solid （3.6） and liquid （4.5） method, and the best plant growth parameter. While, the lower immersion frequency and longer immersion time （30 rain every 12 h and 60 min every 24 h） induced vitrification and pollution rate in shoot tips 16.6% and 19%, 42% and 37%, respectively. There is distinct decrease in pollution rate （8.3%） in TIBS （10 min every 6 h） compared with the conventional semi-solid and liquid cultures. Medium containing 4 mg/L BA and 0.5 mg/L NAA using 10 min immersion every 6 h showed satisfaction at the rooting stage, with high shoot proliferation rate （21.6）, 100% rooting and 94% plant survival. Therefore, applying TIBS in S. sagittifolia is an efficient method for scaling up the production of plantlets with high quality seedlings.

关键词： sagittaria sagittifolia temporary immersion bioreactor system proliferation and growth root formation.

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Extraction and Anti-inflammatory Effects of Colchicine from sagittaria sagittifolia

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Agricultural Science & Technology 2017年第7期18卷 1208-1212,1216页

作者：欧丽兰朱烨税丕先张椿西南医科大学药学院四川泸州646000

[Objective] This study was to study the optimal extraction technology and anti-inflammatory effects of colchicine from sagittaria sagittifolia. [Method] The ef- fects of ethanol concentration, extraction time, extraction temperature and solid-liquid ratio on the extraction rate of colchicine from S. sagittifolia were investigated. On the basis of single-factor experiments, an L9 （34） orthogonal test was carried out to optimize the extraction process. According to the optimal extraction process, the content of colchicine in S. sagittifolia was determined by high performance liquid chromatography. The anti-inflammatory ability of colchicine was evaluated through an anti-inflammatory test in vitro. [Result] The optimal extraction process of colchicine from S. sagittifolia was as follows： ethanol concentration of 60%, extraction temper- ature of 50℃, extraction time of 30 min, and solid-liquid ratio of 1：25 （g/ml）. The content of colchicine in S. sagittifolia was determined as 40.58 μg/100 mg. Com- pared with the control, the colchicine from S. sagittifolia （9.0 and 4.5 g/kg） inhibited the increase in PGE2, TNF-α and IL-1β contents in pleural fluid （P〈0.05）. High-dose colchicine inhibited the increase in TNF-α, IL-1β and MDA contents in lung tissue （P〈0.01）, and middle-dose colchicine inhibited the increase in IL-1β content in lung tissue （P〈0.01）. [Conclusion] The colchicine in S. sagittifolia has a good anti-inflam- matory effect, which may be achieved through hindering the production of inflam- matory mediators and antioxidation.

关键词： sagittaria sagittifolia Colchicine Anti-inflammatory effect

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Effects of “3414” Fertilization Effect Test on the Yield and Economic Benefit of sagittaria sagittifolia

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Agricultural Science & Technology 2017年第6期18卷 1076-1079,1101页

作者：张驰高美萍莫志欢江文韦绍龙何芳练颜梅新平乐县农业局广西平乐542400 广西农业科学院生物技术研究所广西南宁530007

[Objective] To screen the optimal fertilizer formula, and to lay foundation for the research on special fertilizer for sagittaria sagittifolia production. [Method] Based on the ＂3414＂ fertilization design, the yield of S. sagittifolia in each treatment was determined, and the effects of N, P and K treatments on the yield and eco- nomic benefit of S. sagittifolia were investigated, obtaining the optimal fertilizer appli- cation ratio. [Result] N, P and K had relatively good effects on the yield of S. sagittifolia. Treatment of N2P2K3 showed the optimal fertilizer application ratio, with the yield of 18 397.80 kg/hm2, yield increasing rate of 104.77%, and net income in- crease of 80 317.35 Yuan/hm2. N fertilizer had the greatest impacts on the yield of S. sagittifolia, followed with K fertilizer; while P fertilizer showed the poorest impacts. [Conclusion] To achieve the highest yield of S. sagittifolia, the fertilizer application was as follows： N of 446.70 kg/hm2, P20 of 568.10 kg/hm2, K2O of 534.90 kg/hm2, when the highest yield was 18 612.75 kg/hm2. Fertilizer application for the optimal economic benefit was N of 420.60 kg/hm2, P2O5 of 70.50 kg/hm2, K2O of 492.15 kg/hm2, when the optimal yield was 18 579.00 kg/hm2.

关键词： sagittaria sagittifolia "3414" fertilization effect test Yield Economic ben- efit

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