High Efficiency of Propagation for Sagittaria sagittifolia Using a Temporary Immersion Bioreactor System
High Efficiency of Propagation for Sagittaria sagittifolia Using a Temporary Immersion Bioreactor System作者机构：Biotechnology Research Institute Guangxi Academy of Agricultural Sciences No. 174 Daxue Road Nanning Guangxi 530007 China College of Agriculture Guangxi University No. 100 Daxue Road Nanning Guangxi 530007 China Agricultural Bureau in Ping Le Areas Guangxi 542400 China
出 版 物：《Journal of Agricultural Science and Technology(A)》 (农业科学与技术（A）)
年 卷 期：2016年第6卷第3期
页 面：161-170页
学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 090403[农学-农药学(可授农学、理学学位）] 08[工学] 09[农学] 0904[农学-植物保护] 0836[工学-生物工程] 082203[工学-发酵工程] 0822[工学-轻工技术与工程] 083203[工学-农产品加工及贮藏工程]
主 题：Sagittaria sagittifolia temporary immersion bioreactor system proliferation and growth root formation.
摘 要：Although several studies have reported on the propagation of the Sagittaria sagittifolia to date, none of these methods have efficiently achieved the mass production of these plants. The present study aimed to investigate the propagation and growth of S. sagittifolia using a temporary immersion bioreactor system （TIBS） compared with conventional semi-solid and liquid culture. The effect of different immersion frequencies and immersion times together with supplementation of various plant growth regulators to the Murashige and Skoog （MS） medium was evaluated on shoot proliferation and plant growth. The results showed that the higher immersion frequency （every 6 h） and shorter immersion time （3 min and 10 rain） in medium containing 4 mg/L BA and 0.1 mg/L NAA produced the highest multiplication rate （23）, which are significantly higher than conventional semi-solid （3.6） and liquid （4.5） method, and the best plant growth parameter. While, the lower immersion frequency and longer immersion time （30 rain every 12 h and 60 min every 24 h） induced vitrification and pollution rate in shoot tips 16.6% and 19%, 42% and 37%, respectively. There is distinct decrease in pollution rate （8.3%） in TIBS （10 min every 6 h） compared with the conventional semi-solid and liquid cultures. Medium containing 4 mg/L BA and 0.5 mg/L NAA using 10 min immersion every 6 h showed satisfaction at the rooting stage, with high shoot proliferation rate （21.6）, 100% rooting and 94% plant survival. Therefore, applying TIBS in S. sagittifolia is an efficient method for scaling up the production of plantlets with high quality seedlings.
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