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Reversibly immortalized keratinocytes(iKera)facilitate re-epithelization and skin wound healing:Potential applications in cell-based skin tissue engineering

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Bioactive Materials 2022年第3期7卷 523-540页

作者： Jiamin Zhong Hao Wang Ke Yang Huifeng Wang Chongwen Duan Na Ni Liqin An Yetao Luo Piao Zhao Yannian Gou Shiyan Sheng Deyao Shi Connie Chen William Wagstaff b Bryce Hendren-Santiago b Rex C.Haydon b Hue H.Luu b Russell R.Reid Sherwin HHo Guillermo A.Ameer Le Shen tong-Chuan He Jiaming Fan Ministry of Education Key Laboratory of Diagnostic Medicine And Department of Clinical BiochemistrySchool of Laboratory MedicineChongqing Medical UniversityChongqing400016China Molecular Oncology Laboratory Department of Orthopaedic Surgery and Rehabilitation MedicineThe University of Chicago Medical CenterChicagoIL60637USA the Pediatric Research Institute The Children’s Hospital of Chongqing Medical UniversityChongqing400014China Biomedical Engineering Department Northwestern UniversityEvanstonIL60208USA Department of Orthopaedics Union Hospital of Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina Department of Surgery The University of Chicago Medical CenterChicagoIL60637USA Center for Advanced Regenerative Engineering(CARE) EvanstonIL60208USA Department of Surgery Northwestern University Feinberg School of MedicineChicagoIL60616USA

Skin injury is repaired through a multi-phase wound healing process of tissue granulation and *** failure in the healing process may lead to chronic non-healing wounds or abnormal scar *** significant progress has been made in developing novel scaffolds and/or cell-based therapeutic strategies to promote wound healing,effective management of large chronic skin wounds remains a clinical *** are critical to re-epithelialization and wound ***,we investigated whether exogenous keratinocytes,in combination with a citrate-based scaffold,enhanced skin wound *** first established reversibly immortalized mouse keratinocytes(iKera),and confirmed that the iKera cells expressed keratinocyte markers,and were responsive to UVB treatment,and were *** a proof-of-principle experiment,we demonstrated that iKera cells embedded in citrate-based scaffold PPCN provided more effective re-epithelialization and cutaneous wound healing than that of either PPCN or iKera cells alone,in a mouse skin wound ***,these results demonstrate that iKera cells may serve as a valuable skin epithelial source when,combining with appropriate biocompatible scaffolds,to investigate cutaneous wound healing and skin regeneration.

关键词： Keratinocytes Skin tissue engineering Reversible immortalization sv40 large t antigen PPCN Skin wound healing

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Gene expression by simian virus 40 large tantigen-induced medulloblastomas in mice

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Neural Regeneration Research 2012年第12期7卷 932-937页

作者： Xiaoluan Wei Jie Feng Yinghe Hu Shanghai Engineering Research Center of Molecular therapeutics and New Drug Development Key Laboratory of Brain Functional Genomics MOE ＆ STCSM East China Normal University Shanghai 200062 China Shanghai Laboratory Animal Research Center Shanghai 200032 China

Signaling pathways known to have components with mutations in human medulloblastoma include sonic hedgehog, Wnt/beta-catenin and insulin-like growth factor. Microarray analysis was applied to examine the gene expression changes in medulloblastomas of ptet-on/ptRE-sv40tag transgenic mice. Altogether, 14 112 genes were detectable, including 152 genes with significantly different expression levels. these genes were associated with immunity, the cell cycle, signal transduction, cytoskeleton and metabolism. to further confirm the microarray data, real-time polymerase chain reactions were used to examine the expression changes of genes related to sonic hedgehog, Wnt/beta-catenin and insulin-like growth factor signal pathways. Immunohistochemistry detected insulin receptor substrate-1 in the nuclei of brain tumor tissue cells from ptet-on/ptRE-sv40tag transgenic mice, suggesting that sv40 large t antigen may activate the insulin-like growth factor signal pathway to promote tumorigenesis.

关键词： sv40 large t antigen medulloblastoma cDNA microarray real-time polymerase chain reaction insulin-like growth factor

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Establishment of cell lines with porcine spermatogonial stem cell properties

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Journal of Animal Science and Biotechnology 2020年第3期11卷 678-689页

作者： Yi Zheng tongying Feng Pengfei Zhang Peipei Lei Fuyuan Li Wenxian Zeng Key Laboratory for Animal Genetics Breeding and Reproduction of Shaanxi ProvinceCollege of Animal Science and TechnologyNorthwest A&F UniversityYangling 712100ShaanxiChina

Background:Spermatogonial stem cells(SSCs)are capable of both self-renewal and differentiation to mature functional spermatozoa,being the only adult stem cells in the males that can transmit genetic information to the next *** SSCs hold great value in transgenic pig production and in establishment of porcine models for regenerative ***,studies and applications of porcine SSCs have been greatly hampered by the low number of SSCs in the testis as well as the lack of an ideal stable long-term culture system to propagate porcine SSCs ***:In the present study,by lentiviral transduction of plasmids expressing the simian virus 40(sv40)large t antigen into porcine primary SSCs,we developed two immortalized cell lines with porcine SSC *** established cell lines,with the expression of porcine SSC and germ cell markers UCHL1,PLZF,tHY1,VASA and DAZL,could respond to retinoic acid(RA),and could colonize the recipient mouse testis without tumor formation after *** cell lines displayed infinite proliferation potential,and have now been cultured for more than 7 months and passaged for over 35 times without morphological ***:We have for the first time established porcine SSC lines that could provide abundant cell sources for mechanistic studies on porcine SSC self-renewal and differentiation,thereby facilitating development of an optimal long-term culture system for porcine primary SSCs and their application to animal husbandry and medicine.

关键词： Immortalization Pig Self-renewal Spermatogonial stem cells sv40 large t antigen

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