Establishment of cell lines with porcine spermatogonial stem cell properties

作     者：Yi Zheng Tongying Feng Pengfei Zhang Peipei Lei Fuyuan Li Wenxian Zeng Yi Zheng;Tongying Feng;Pengfei Zhang;Peipei Lei;Fuyuan Li;Wenxian Zeng

作者机构：Key Laboratory for Animal GeneticsBreeding and Reproduction of Shaanxi ProvinceCollege of Animal Science and TechnologyNorthwest A&F UniversityYangling 712100ShaanxiChina 

出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)

年 卷 期：2020年第11卷第3期

页      面：678-689页

核心收录：

学科分类：0905[农学-畜牧学] 09[农学] 090501[农学-动物遗传育种与繁殖] 

基　　金：This study was supported by the National Natural Science Foundation of China(Grant No.31572401,31772605)to W.Z the Open Fund of Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province(Grant No.SNDK-KF-201804) Young Talent fund of University Association for Science and Technology in Shaanxi,China(Grant No.20180204)and a startup fund from Northwest A&F University(Grant No.2452018037)to Y.Z 

主　　题：Immortalization Pig Self-renewal Spermatogonial stem cells SV40 large T antigen 

摘      要：Background:Spermatogonial stem cells(SSCs)are capable of both self-renewal and differentiation to mature functional spermatozoa,being the only adult stem cells in the males that can transmit genetic information to the next *** SSCs hold great value in transgenic pig production and in establishment of porcine models for regenerative ***,studies and applications of porcine SSCs have been greatly hampered by the low number of SSCs in the testis as well as the lack of an ideal stable long-term culture system to propagate porcine SSCs ***:In the present study,by lentiviral transduction of plasmids expressing the simian virus 40(SV40)large T antigen into porcine primary SSCs,we developed two immortalized cell lines with porcine SSC *** established cell lines,with the expression of porcine SSC and germ cell markers UCHL1,PLZF,THY1,VASA and DAZL,could respond to retinoic acid(RA),and could colonize the recipient mouse testis without tumor formation after *** cell lines displayed infinite proliferation potential,and have now been cultured for more than 7 months and passaged for over 35 times without morphological ***:We have for the first time established porcine SSC lines that could provide abundant cell sources for mechanistic studies on porcine SSC self-renewal and differentiation,thereby facilitating development of an optimal long-term culture system for porcine primary SSCs and their application to animal husbandry and medicine.