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Synergistic activity of lawsone methyl ether in combination with some antibiotics and artocarpin against methicillin-resistant Staphylococcus aureus, Candida albicans, and Trychophyton rubrum

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Chinese Herbal Medicines 2019年第3期11卷 321-325页

作者： Pharkphoom Panichayupakaranant Abdi Wira Septama Akarawat Sinviratpong Department of Pharmacognosy and Pharmaceutical Botany Faculty of Pharmaceutical SciencesPrince of Songkla UniversityHat-YaiSongkhla 90112Thailand Phytomedicine and Pharmaceutical Biotechnology Excellence Center Faculty of Pharmaceutical SciencesPrince of Songkla UniversityHat-YaiSongkhla 90112Thailand Research Center for Chemistry Indonesian Institute of SciencesKawasan PUSPIPTEK SerpongTangerang SelatanBanten 15314Indonesia

Objective: One appealing strategy to overcome and prevent resistant problem is the use of combined two or more antibacterial substances. Lawsone methyl ether(LME) is the naphthoquinone found in the leaves of Impatiens balsamina. The objective of this study is to determine the interaction of LME with some antibiotics(ampicillin, tetracycline, norfloxacin, and clotrimazole) and a natural compound, artocarpin against methicillin-resistant Staphylococcus aureus(MRSA), Candida albicans, and Trychophyton ***: A broth microdilution method was used to determine the minimum inhibition concentration(MIC). Synergistic effects were evaluated at their own MIC using the checkerboard method and time-kill ***: LME showed moderate antibacterial activity against MRSA with MIC value of 15.6 μg/mL, and exhibited strong antifungal activities against T. rubrum and C. albicans with MIC values of 7.8 and 3.9 μg/m L,respectively. The interaction of LME with the natural compound artocarpin against MRSA produced a synergy with fractional inhibitory concentration index(FICI) value of 0.31, while the combination of LME and clotrimazole exhibited synergy against C. albicans and T. rubrum with FICI values of 0.38 and 0.24, respectively. The time-kill assays confirmed that the compounds in combination enhanced their antimicrobial activities against the resistant microorganisms with different ***: LME in combination with clotrimazole exhibited synergy effect against C. albicans and ***. In combination with artocarpin, it showed synergy effect against MRSA.

关键词： artocarpin Candida albicans clotrimazole lawsone methyl ether Staphylococcus aureus synergy Trychophyton rubrum

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Economic Value Evaluation of Fine Individual Plants of Ribes rubrum Linn. Based on AHP

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Asian Agricultural Research 2019年第2期11卷 102-105页

作者： Yu CHEN Yulan GUO Kewu LIU Forest By-product and Speciality Institute in Heilongjiang Province

In this experiment,the biological characteristics,the number of spikes per plant,the average number of panicles,the average spike length,and the average yield per plant of the wild Ribes rubrum Linn. were observed and determined and the analytic hierarchy process( AHP)was applied to evaluate the economic value of 8 different sources of excellent plants of R. rubrum Linn. The results indicated that " RXF02", " RXF03" and " RXM05" performed well in the number of spikes per plant,average number of panicles,average yield per plant,and cold resistance,and the comprehensive scores were 9. 68,9. 44 and 9. 41,respectively.

关键词： Ribes rubrum Linn. Analytic hierarchy process (AHP) Economic value evaluation

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Human urine metabolome in response to Fermentum rubrum(Hongqu Capsules)by HILIC-UHPLC-QTOF

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中国药理学与毒理学杂志 2015年第S1期29卷 106-106页

作者： HongYANG EdmundJ.D.LEE SeokHweeKOO FengTIAN School of Applied Science Temasek Polytechnic Singapore 529757 Department of Pharmacology Yong Loo Lin School of Medicine National University of Singapore Singapore 117597 Clinical Trials & Research Unit Changi General Hospital Singapore 529889

OBJECTIVE ″-omics″study represents an unbiased perspective to examine the bio-system to discover the novel biomarker(s)which might be overlooked when targeted analysis is performed ***,due to its ease of collection,minimal invasion involved and rich information of the downstream metabolites,has been extensively *** rubrum(Hongqu,HQ capsules)is a well-known traditional medicine with the claimed slimming effect which could be related to the reduction in the deposition of total cholesterol and *** in sufficient clinical evidence is always one of the leading reasons that hamper traditional medicines from gaining world-wide *** our pursuit of scientific support for HQ capsules in managing obesity,we aim to examine the alteration of urinary metabolites in this small-scale human clinical *** 6 Chinese subjects were included and exposed to short-term administration of HQ capsules for 3d,twice daily,two capsules each *** urine samples were collected for three days prior to the dosing and on the day following the last *** total,96 urine samples were collected and then separated on hydrophilic interaction chromatography(HILIC)and analyzed by Agilent 6550 ESI-Quadrupoletime-of-flight(QTOF)mass *** Under positive mode,two compounds were found to be present only in post-dosing urine,and one compound was significantly lowered in post-dosing *** two compounds might be associated with the administered HQ capsules,which is formulated with multiherbal *** Further elucidation on the structures of these compounds is needed to enable better understanding of the mechanism of HQ capsules in managing obesity.

关键词： urine metabolome Fermentum rubrum HILIC-UHPLC-QTOF

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枫叶(Acer rubrum L.)引起的幼驹海恩茨氏体性贫血和高铁血红蛋白血症

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黑龙江畜牧兽医 1985年第2期 42-43页

作者：丁岚峰于泽源

用干燥的枫树叶按3克/公斤体重饲喂幼驹后,1～5天死亡。临床经过大致区分为二个方面:9月15日以后采集、并且干燥的枫树叶饲喂幼驹后第18小时急死;而用9月15日以前采集且干燥的枫树叶饲喂幼驹,则呈现溶血性症候群,饲喂后3～5天死亡。用... 详细信息

用干燥的枫树叶按3克/公斤体重饲喂幼驹后,1～5天死亡。临床经过大致区分为二个方面:9月15日以后采集、并且干燥的枫树叶饲喂幼驹后第18小时急死;而用9月15日以前采集且干燥的枫树叶饲喂幼驹,则呈现溶血性症候群,饲喂后3～5天死亡。用采集后未干燥的枫叶饲喂幼驹则不发病,枫叶经过干燥才出现毒

关键词：幼驹饲喂枫树叶干燥饲养化工单元操作 rubrum Acer 枫叶

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IBA和NAA对美国红枫扦插生根的影响

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湖北农业科学 2015年第8期54卷 2013-2015,2020页

作者：高焕章赵振军何玉枝丁亚琴长江大学园艺园林学院湖北荆州434025 鄂州职业大学艺术与传媒学院湖北鄂州436000

研究了IBA和NAA对美国红枫（Acer rubrum L．）扦插生根的影响。结果表明．美国红枫扦插不定根形成大体分为3个时期，0～7d为不定根根原基诱导期；7，11d为不定根形成期：11～25d为不定根伸长期。50mg／L IBA＋100mg/LNAA组合效果最好... 详细信息

研究了IBA和NAA对美国红枫（Acer rubrum L．）扦插生根的影响。结果表明．美国红枫扦插不定根形成大体分为3个时期，0～7d为不定根根原基诱导期；7，11d为不定根形成期：11～25d为不定根伸长期。50mg／L IBA＋100mg/LNAA组合效果最好，生根率、生根数、根长、根系效果指数分别为86．74％、30．67条、6．99cm、8．36。生根率差异主要是由于IBA的浓度梯度,其次是IBA和NAA两因素间交互作用、NAA的浓度梯度；生根数差异主要是由于NAA的浓度梯度，其次是IBA和NAA两因素间交互作用、IBA的浓度梯度；根长差异主要是由于IBA和NAA两因素间交互作用、IBA的浓度梯度;根系效果指数差异是由于IBA和NAA两因素间交互作用、IBA的浓度梯度、NAA的浓度梯度。

关键词：美国红枫(Acer rubrum L.) IBA NAA 组合溶液扦插生根

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Therapeutic Effects of Essential Oil from Waste Leaves of <i>Psidium guajava</i>L. against Cosmetic Embarrassment Using Phylogenetic Approach

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American Journal of Plant Sciences 2012年第6期3卷 745-752页

作者： Ahsan Kamran Rohit Mishra Rita Gupta Awadhesh Kumar Ashok Bajaj Anupam Dikshit Biological Product Laboratory Department of Botany University of Allahabad Allahabad India Department of Dermatology and Veneral Diseases M.L.N.Medical College Allahabad India. Department of Horticulture Aromatic & Medicinal Plants Mizoram University Aizawl India

Medicinal plants are facing threats due to loss of habitat and overexploitation of knows species. Therefore the present work shows utilization of essential oil from discarded leaves of Psidium guajava Linn. against human dermatophytic fungi Trichophyton rubrum, T. mentagrophytes, Trichophyton violaceum, Trichophyton tonsurans, Epidermophyton floccosum, Microsporum gypseum and Microsporum canis. The oil was found to be fungicidal at 3.0 μl/ml and it tolerated heavy inoculum of pathogens at fungicidal concentrations. The fungicidal activity of the oil was thermostable, up to 70°C and shelf life was found to be six months, which was maximum the time taken into consideration. The oil having cineole, caryophyllane, copaene, azulene and eucalyptol as main constituents, exhibited broad fungicidal activity. The oil did not show any adverse effect on mammalian skin upto 5% concentration. Phylogeny of the dermatophytes with respect to toxicity of the oil has also been discussed using molecular data. The cost effective formulation from waste leaves can be exploited after undergoing double blind successful multicentral topical testing.

关键词： Essential Oil Dermatophytes Phylogeny Discarded Leaves Psidium guajava L. Trichophyton rubrum T. mentagrophyte

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Dermatophytes Species Isolated of HIV-Infected Patients Identified by ITS-RFLP and ITS Region Sequencing from Triangulo Mineiro, Minas Gerais State of Brazil

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Advances in Microbiology 2019年第9期9卷 790-803页

作者： Thatiana Bragine-Ferreira Luiz Sé rgio de Lima-Jú nior Larissa Beatriz Silva Leonardo Eurí pedes Andrade-Silva Delio José Mora Bruno Siqueira Prudente Kennio Ferreira-Paim Fabiana Almeida Araú jo Santos Luiz Ricardo Goulart Mario Leó n Silva-Vergara Infectious Diseases Unit Internal Medicine Department Triangulo Mineiro Federal University Uberaba Brazil Department of Microbiology Tri&acire ngulo Mineiro Federal University Uberaba Brazil Laboratory of Nanobiotechnology Institute of Biotechnology Federal University of Uberl&acire ndia Uberl&acire ndia Brazil

Dermatomycoses are a group of diseases worldwide distributed and affect skin, hair and nails. Several fungal species can be envolved and keratinophilic fungi belonging to the genera Trichophyton, Microsporum and Epidermophyton are very common. These infections are globally distributed and their incidence has progressively increased. Despite their high incidence, few studies have been carried out in HIV-infected patients, regarding frequency, clinical presentations, and species identification. This study aimed to evaluate some epidemiological and clinical aspects of dermatophytosis in HIV-infected patients and to attempt phenotypical and molecular characteristics of their agents. Of 398 patients included, 306 were HIV-infected. Clinical data and samples of skin, hair and nail lesions were simultaneously obtained. The dermatophytes yielded in culture were phenotypically identified and evaluated by ITS-RFLP and ITS (Internal Transcribed Space) sequencing. Dermatophytes frequency in HIV-infected patients with cutaneous lesions was 11.76% similar to the 15.22% observed in non-HIV individuals. Trichophyton rubrum was the most common isolated species in both groups. Throught ITS-RFLP, 29/32 (90.63%) of dermatophytes isolates were identified at the species level. From the 10 isolates randomly selected for ITS sequencing, seven confirmed the ITS-RFLP results while three Trichophyton sp. were not identified as dermatophytes. The identification of these species in HIV-infected patients is highly desirable in order to improve the knowledge of their frequency, geographical distribution and relation to clinical presentation.

关键词： Dermatophytosis Dermatophytes ITS-RFLP ITS-Sequencing HIV-Infected Patients Trichophyton rubrum

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Influence of Metal Ions on Hydrogen Production by Photosynthetic Bacteria Grown in Escherichia coli Pre-Fermented Cheese Whey

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Journal of Environmental Protection 2010年第4期1卷 426-430页

作者： Fadhil M. Salih Muthana I. Maleek 不详

The photosynthetic bacteria, Rodospirillum rubrum, produced hydrogen when grown in cheese whey in presence of light. The production increased three times as much when whey was used after being incubated in presence of Escherichia coli at 37℃ for 5 days, giving a total of 3600 ml of H2 in 10 days. The presence of Fe ions (5 mg/L) enhanced H2 production of treated whey to about 6000 ml in 10 days. Mo ions (0.3 and 1.6 mg/l) did not affect achieved H2 production of treated whey. However, when Fe and Mo ions were present together, the production was comparable with that of Mo ions alone, i.e. Mo prevented Fe of producing any enhancing effect. The addition of Mn ions (7.68 mg/L) to treated whey containing Fe (5 mg/L) and Mo ions (8 mg/L) increased H2 production to give about 9500 ml/10 days.

关键词： Hydrogen Production Photosynthetic Bacteria Rodospirillum rubrum Metal Ions E. coli Fermentation

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Anti-yeast activities of Origanum oil against human pathogenic yeasts

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Advances in Bioscience and Biotechnology 2011年第2期2卷 103-107页

作者： Amber Adams Satyanshu Kumar John Clauson Shivendra Sahi 不详

Outbreak of autoimmune diseases by pathogenic yeasts has led to a serious medical threat. As these organisms evolve resistance to existing antifungal drugs, the concern could be further compounded. The realm of plant derived products offers a wide spectrum of potentially valuable alternatives to the existing synthetic fungicides. Essential oils from sev-eral medicinal plants have been shown to exhibit pharmacological attributes. In the present study, anti-yeast properties of Oregano essential oil (OEO) were examined in vitro against four human patho-genic yeasts i.e., Candida albicans, Cryptococcus al-bidus, Cryptococcus neoformans and Rhodotorula ru-brum. OEO concentration of 200 μg/mL was found to be growth inhibitory against all four yeasts examined, thereby showing its potential to function as a natural anti-yeast agent.

关键词： Origanum Vulgare Candida Albicans Cryptococcus Neoformans Rhodotorula rubrum Cryptococcus Albidus

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加拿大红枫组织培养条件的优化

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湖北农业科学 2012年第17期51卷 3872-3875页

作者：胡雪雁胡业华管倩连方青陈怡芳江西科技师范大学生命科学学院南昌330013 江西农业大学园林与艺术学院南昌330046

以加拿大红枫(Acer rubrum L.)的顶芽和侧芽为外植体,考察不同消毒剂处理时间对消毒效果的影响,以及植物生长调节剂及其浓度配比对愈伤组织诱导及不定芽增殖的影响。结果表明,外植体的最佳采集时期为4月中旬;先用体积分数70%的乙醇处理1... 详细信息

以加拿大红枫(Acer rubrum L.)的顶芽和侧芽为外植体,考察不同消毒剂处理时间对消毒效果的影响,以及植物生长调节剂及其浓度配比对愈伤组织诱导及不定芽增殖的影响。结果表明,外植体的最佳采集时期为4月中旬;先用体积分数70%的乙醇处理15 s,再用1 mg/mL HgCl2处理7 min,最后用20mg/mL NaClO浸泡25 min,加拿大红枫外植体的消毒效果好,且存活率最高,为90%。诱导加拿大红枫愈伤组织的适宜培养基为1/2 MS+2.0 mg/L 6-BA+0.5 mg/L 2,4-D+0.1 mg/L NAA+0.1 mg/L GA3。较适合加拿大红枫不定芽增殖的培养基为1/2 MS+1.0 mg/L 6-BA+0.1 mg/L NAA+0.1 mg/L GA3。

关键词：加拿大红枫(Acer rubrum L.) 愈伤组织不定芽诱导

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