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Effects of rosmarinic acid on DNA Integrity and H19 Differentially Methylated Region Methylation Levels in Human Sperm Preserved by Freeze-Drying

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Reproductive and Developmental Medicine 2021年第1期5卷 9-14页

作者： Yi-Yu Wang Wei-Jie Zhu Department of Developmental and Regenerative Biology College of Life Science and TechnologyJinan UniversityGuangzhou 510632China

Original Article Effects of rosmarinic acid on DNA Integrity and H19 Differentially Methylated Region Methylation Levels in Human Sperm Preserved by Freeze-Drying Wang Yi-Yu,Zhu Wei-Jie Published 2021-03-25 Cite as Reprod Dev Med,2021,05 Reprod Dev Med,2021,05(1):***:10.4103/2096-2924.309790 Abstract Objective:To investigate the effects of rosmarinic acid(RA)on the DNA integrity and methylation levels of the H19 differentially methylated region(DMR)of freeze-dried human sperm after 1 week and 6 months of storage at 4℃.Methods:Semen samples from 15 healthy normospermic donors were used in this *** samples were divided into five groups,including the control group with fresh sperm and four experimental groups with freeze-dried sperm(1-week storage with EGTA buffer solution,Group A;1-week storage with EGTA buffer solution containing 105μmol/L RA,Group B;6-month storage with EGTA buffer solution,Group C;and 6-month storage with EGTA buffer solution containing 105μmol/L RA,Group D).DNA integrity was evaluated using the sperm chromatin dispersion test.H19 DMR methylation levels were detected by bisulfite sequencing polymerase chain ***:After 1 week of storage,no differences in sperm DNA integrity were observed among Groups A,B,and controls(P>0.05).After 6 months of storage,the sperm DNA integrity of Group D did not change significantly compared with that of the control group(P>0.05),whereas that of Group C decreased significantly(P0.05).Conclusions:The DNA integrity of freeze-dried human sperm can be effectively protected by adding RA within 6 months,and the H19 DMR methylation level of human sperm can be maintained for 6 months after freeze-drying.

关键词： DNA Integrity DNA Methylation Freeze-Drying Human Sperm rosmarinic acid

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Chemical Fingerprint and Anti-Sickling Activity of rosmarinic acid and Methanolic Extracts from Three Species of Ocimum from DR Congo

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Journal of Biosciences and Medicines 2016年第1期4卷 59-68页

作者： Dorothée D. Tshilanda Paulin K. Mutwale Damase V. N. Onyamboko Philippe B. Babady Philippe V. Tsalu Damien S. T. Tshibangu Nadege K. Ngombe M. Frederich Koto-te-Nyiwa Ngbolua Pius T. Mpiana Department of Chemistry Faculty of Science University of Kinshasa Kinshasa Democratic Republic of the Congo Centre d’Etudes des Substances Naturelles d’Origine Végétale (CESNOV) Faculty of Pharmaceutical Sciences University of Kinshasa Kinshasa Democratic Republic of the Congo Bila Nutraceuticals Company Dartmouth Canada Laboratory of Pharmacognosy Department of Pharmacy CIRM University of Liège CHU Liège Belgium Department of Biology Faculty of Science University of Kinshasa Kinshasa Democratic Republic of the Congo

The aim of this study was to characterize the polyphenolic composition by determination of chemical fingerprints of Methanolic extracts of Ocimum canum Sims, Ocimum basilicum L. and Ocimum gratissimum L. from Democratic Republic of Congo and to compare their antisickling activity of that of rosmarinic acid, the major compound to those of methanolic extracts. Phytochemical analysis performed by TLC and HPLC analysis, showed that rosmarinic acid is the most abundant phenolic acid in these Ocimum species according to the following order O. basilicum L., O. gratissimum L. and O. canum Sims. Methanolic extracts of these three species and pure rosmarinic acid showed significant antisickling activities with minimal concentration of normalization values of 0.18 ± 0.03, 0.23 ± 0.04, 0.26 ± 0.04 and 0.31 ± 0.05 mg/mL for rosmarinic acid, O. basilicum L., O. gratissimum L. and O. canum Sims methanolic extracts respectively. The antisickling activity order is the same as that of the rosmarinic acid content, indicating that this polyphenolic acid would be among the main active molecules in these extracts.

关键词： Antisickling Chemical Fingerprint O. canum O. basilicum O. gratissimum rosmarinic acid

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Investigation of the Effect of rosmarinic acid on Cyclophosphamide-Induced Gonadal Toxicity

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Advances in Sexual Medicine 2022年第1期12卷 1-8页

作者： Fırat Şahin Fırat Aşır Ebru Gökalp Özkorkmaz Süreyya Özdemir Başaran Özge Kaplan Işılay Sezen Ermiş Engin Deveci Department of Histology and Embryology Faculty of Medicine Dicle University Diyarbakir Turkey

The aim of this study was to investigate the effect of rosmarinic acid against gonadal toxicity caused by cyclophosphamide, an important anticancer drug. A total of 28 rats were divided into 4 groups, with 7 animals in each group. The groups were created as follows;Group 1 (control) (n = 7): Subjects received only 1 ml of 0.9% saline solution per day intraperitoneally for 14 days. Group 2 (Rosmarinic Acid) (n = 7): The subjects were given 20 mg/kg Rosmarinic acid intraperitoneally for 14 days. Group 3 (Cyclophosphamide) (n = 7): Only 1 ml of 0.9% saline solution was administered intraperitoneally to the subjects for the first 7 days. Cyclophosphamide 20 mg/kg per day was administered intraperitoneally for the last 7 days (from the 8th day). Group 4 (Rosmarinic Acid + Cyclophosphamide) (n = 7): The subjects were given 20 mg/kg Rosmarinic acid daily intraperitoneally for 14 days. From the 8th day of the experiment to the end of the experiment (last 7 days of the experiment), 20 mg/kg cyclophosphamide was given intraperitoneally daily. At the end of the experiment, body weights of all rats were measured first. Afterwards, the weights of testicular tissue samples were measured and the averages of the weights were taken. Routine tissue follows up was performed on the testicular tissues taken. Hematoxylin-eosin staining was applied to tissue sections of 5 μm thickness. As a result of the statistical analysis, it was determined that cyclophosmadine decreased body and testicular weight, but rosmarinic acid had a protective effect on the contrary. It was concluded that cyclophosmadine caused damage t

关键词： Testis Cyclophosphamide rosmarinic acid Sertoli Cell Hematoxylin Eosin

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Neuroprotective effects of rosmarinic acid in the MPTP mouse model of Parkinson’s disease

Neuroprotective effects of rosmarinic acid in the MPTP mouse...

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中国生理学会第24届全国会员代表大会暨生理学学术大会

作者：贾文婷 Hua-Min XU Hong JIANG Jun-Xia XIE Department of Physiology Shandong Provincial Key Laboratory of Pathogenesis and Prevention of Neurological Disorders and State Key Disciplines:PhysiologyQingdao University

Parkinson’s disease（PD）is a common neurodegenerative disease characterized by the selective loss of dopaminergic neurons in substantia nigra（SN）.rosmarinic acid（RA）is a naturally occurring polyphenolic compound found in herbs,and has multiple biological activities such as antioxidative,anti-inflammatory and antiviral *** this study,we investigated the neuroprotective effects of RA on dopaminergic neurons in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine（MPTP）-induced C57BL/6 mouse model of *** showed that the levels of dopamine and its metabolites in the striatum decreased in MPTP-induced PD *** mice received oral administration of RA before MPTP injection elevated the striatal levels of dopamine and its ***,the tyrosine hydroxylase expression in the SN decreased in MPTP intoxicated PD *** could be significantly inhibited by pretreatment with *** addition,RA could restore the decrease of SOD induced by *** study suggests that RA could suppress MPTP-induced degeneration of the nigrostriatal dopaminergic system by antioxidative ***,RA should be clinically evaluated for the prevention of neurodegenerative diseases.

关键词： rosmarinic acid Parkinson’s disease MPTP Neuroprotective

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Protective effects of rosmarinic acid on iron induced toxicity in SK-N-SH cells

Protective effects of Rosmarinic acid on iron induced toxici...

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中国神经科学学会第十届全国学术会议

作者： Le QU Hua-Min XU Hong JIANG Jun-Xia XIE Department of Physiology Shandong Provincial Key Laboratory of Pathogenesis and Prevention of Neurological Disorders and State Key Disciplines:PhysiologyMedical College of Qingdao University

Objective rosmarinic acid(RA) is a naturally occurring polyphenolic *** is found in several herbs in the Lamiaceae *** previously reported that RA exerted protective effects against6-hydroxydopamine(6-OHDA) and l-memyl-4-phenylpyridinium(MPP+)-induced neurotoxicity through antioxidative *** this study,we investigated whemer RA could prevent iron-induced toxicity in SK-N-SH *** MTT assay was used to observe the toxicity of *** transmembrane potential(△Ψm) was measured by flow cytometry using rhodamine *** was used to observe the aggregation of ***(1) RA(10

关键词： rosmarinic acid iron Parkinson’s disease

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De novo biosynthesis of rosmarinic acid in Saccharomyces cerevisiae

De novo biosynthesis of rosmarinic acid in Saccharomyces cer...

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中国化学会第十二届全国天然有机化学学术会议

作者： Lijun Geng Yingpeng Xu Yuan Chen J.Andrew Jones Lu Yu Zhengtao Wang Mattheos A.G.Koffas Shujuan Zhao The SATCM Key Laboratory for New Resources & Quality Evaluation of Chinese Medicine The MOE Key Laboratory for Standardization of Chinese Medicines and Shanghai Key Laboratory of Compound Chinese MedicinesInstitute of Chinese Materia MedicaShanghai University of Traditional Chinese Medicine Department of Chemical and Biological Engineering Department of Biological SciencesRensselaer Polytechnic Institute

rosmarinic acid(RA) and its derivatives like salvianolic acid B are water-soluble phenolic acids original from medicinal plants such as Salvia miltiorrhiza with a variety of pharmacological activities .These compounds have been widely used as medicine and food *** we reported the de novo biosynthesis of RA in engineered Saccharomyces cerevisiae through recreating a six-step minis biosynthetic pathway linked tyrosine to *** recreated pathway containing seven genes devided into two ***,4 CL,HPPR,and RAS consisted the first module and were assembled in plasmid *** coupled with CYP and CPR were the second module and constructed into ***424-TAT-4 CL-HPPR-RAS and pRS426-CYP-CPR-TAL were co-transformed into yeast to do two-step fermentation in synthetic dropout(SD) selection ***-MS results indicated RA was produced at 7.9±0.3 mg/*** work demonstrates that de novo biosynthetic RA pathway has been successfully recreated in *** which sheds light on an alternative source for the biosynthesis of RA and its derivatives such as salvianolic acid B and other phenolic acid compounds.

关键词： rosmarinic acid Saccharomyces cerevisiae biosynthesis fermentation

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Effects of main active components of rosemary on growth performance,meat quality and lipid metabolism in finishing pigs

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Animal Nutrition 2023年第4期15卷 341-349页

作者： Qianjin Zhang Jiatai Gong Hongkun Xiang Ruizhi Hu Xizi Yang Jing Lv Wentao Zhang Ming Liu Xiong Deng Xupeng Yuan Ziyu He Yixuan Jiang Bie Tan Jianhua He Shusong Wu Hunan Collaborative Innovation Center for Utilization of Botanical Functional Ingredients College of Animal Science and TechnologyHunan Agricultural UniversityChangsha 410128China Animal Science and Technology College Beijing University of AgricultureBeijing 102206China College of Animal Science and Technology Hunan Biological and Electromechanical PolytechnicChangsha 410127China Department of Food Science and Biotechnology Faculty of AgricultureKagoshima UniversityKagoshima 890-0065Japan Hunan Delore Group Co.Ltd. Changsha 410131China

Rosemary extracts have been widely used as feed additives in recent *** study aimed to investigate the effects of rosmarinic acid(RA)and ursolic acid(UA),the main active components of rosemary,on growth performance,meat quality and lipid metabolism in finishing pigs.A total of 72 finishing pigs(Landrace;initial age of 150 d)were randomly divided into 3 treatments with 8 replicates of 3 pigs each,and fed a basal diet or diet containing 500 mg/kg of RA or *** results showed that dietary supplementation of RA or UA had no significant effect on the growth performance and carcass traits of finishing pigs(P>0.05).However,both RA and UA significantly increased the triglyceride(TG)level in soleus muscle(Pacid synthase(FAS)(Pacid transport protein 1(FATP1),a gene related to lipid uptake(P<0.05).However,RA reduced the expression of adipogenesis-related gene acetyl-coenzyme A carboxylase a(ACCa)(P<0.01).Characterization of cecal microbiota indicated that RA increased the microbial richness(chao 1,P<0.001)and diversity(observed species,P<0.01).Further analysis of the genera revealed that RA increased the relative abundance of Bacteroides and g-UCG-005(P<0.05),and UA enriched Prevotella(P<0.001).Correlation analysis showed that g-UCG-005 was positively correlated with the expression of FAS,carnitine palmitoyl transferase 1B(CPT1B),SREBP1c and PPARγ(P<0.01).In conclusion,dietary supplementation of RA or UA may increase fat deposition in muscle of finishing pigs by regulating lipid metabolism and gut microbiota.

关键词： rosmarinic acid Ursolic acid Finishing pig Lipid metabolism Gut microbiota

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Polyphenol composition and antioxidant potential of mint leaves

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食物生产加工与营养(英文) 2019年第1期1卷 128-141页

作者： Nicole Brown Jenny AJohn Fereidoon Shahidi Department of Biochemistry Memorial University of NewfoundlandSt.John’sNL A1B 3X9Canada Present Address:Department of Food Science and Technology Kerala University of Fisheries and Ocean StudiesKochiKerala 682506India

Soluble and insoluble/bound phenolic compounds and an aqueous infusion of two mint species(Medina and Hasawi),were tested for their total phenolic content,2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonate(ABTS)and 1,1-diphenyl-2picrylhydrazyl(DPPH)radical scavenging activity,iron(Ⅲ)reduction,iron(Ⅱ)chelating and oxygen radical absorbance capacity(ORAC).Furthermore,the potency of the mint extracts in the inhibition of radical-induced DNA scission,human low-density lipoprotein(LDL)cholesterol oxidation,formation of thiobarbituric acid reactive substances(TBARS)in a cooked ground meat system and LPS(lipopolysaccharide)-stimulated cyclooxygenase-2(COX-2)expression in J774A.1 mouse macrophage cells were *** showed that the soluble phenolics had a higher phenolic content and antioxidant activity than the insoluble-bound extracts and aqueous infusions in most of the *** varieties exhibited notable antioxidant activities and inhibition of LDL cholesterol oxidation,DNA scission and COX-2 gene expression at transcriptional ***,Medina mint was a more potent antioxidant than the Hasawi *** performance liquid chromatography with online tandem electrospray ionization mass spectrometry(HPLC/ESI-MS/MS)analysis of the extracts revealed that rosmarinic acid was the major phenolic compound present in both mint samples.

关键词： Mint Phenolic compounds Antioxidant activity rosmarinic acid HPLC ESI-MS MS

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天然植物和海洋动物、微生物来源的抗痛风和肾间质纤维化活性物质的筛选和作用机制研究

天然植物和海洋动物、微生物来源的抗痛风和肾间质纤维化活性物质...

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作者：尚雁君第二军医大学

学位级别：硕士

痛风和慢性肾病是发病率较高的两种疾病,血尿酸长期的升高是造成痛风的主要原因。而肾脏纤维化是慢性肾病发展的最终结果,因而降低血尿酸和抑制肾纤维化的发展是防治这两种疾病的有效途径。虽然有些西药可以治疗此两种疾病,但长期应用... 详细信息

痛风和慢性肾病是发病率较高的两种疾病,血尿酸长期的升高是造成痛风的主要原因。而肾脏纤维化是慢性肾病发展的最终结果,因而降低血尿酸和抑制肾纤维化的发展是防治这两种疾病的有效途径。虽然有些西药可以治疗此两种疾病,但长期应用会有较大的毒副作用,因而从天然活性物质中寻找这两种疾病的治疗药物有一定的临床意义。本课题的目的就是:从几百个天然植物和海洋动物、微生物来源的化合物中筛选抗痛风和肾间质纤维化的活性物质,并进一步研究其可能的作用机理。方法:利用尿酸生成法和超氧离子生成法,建立黄嘌呤氧化酶的活性测定方法,利用次黄嘌呤和氧嗪酸钾腹腔注射建立小鼠高尿酸血症模型,利用尿酸钠踝关节注射建立大鼠痛风模型,利用大鼠单侧输尿管结扎建立肾间质纤维化模型,分别观察rosmarinic acid、acteoside和renierol体外对黄嘌呤氧化酶的抑制作用;体内对小鼠高尿酸血症、rosmarinic acid大鼠痛风性关节炎症的影响;以及rosmarinic acid对大鼠肾间质纤维化的影响。结果表明,在数百种样品中,renierol、acteoside和rosmarinic acid这三种物质在体外有较强的抑制黄嘌呤氧化酶的作用,其IC分别为:1.85μg·ml,12.25μg·ml和56μg·ml。通过酶抑制动力学试验,表明此三种物质均为黄嘌呤氧化酶的竞争性抑制剂。进一步的体内试验证明,renierol、acteoside和rosmarinic acid能显著抑制由次黄嘌呤和氧嗪酸钾引起的小鼠血尿酸升高。同时rosmarinic acid还能明显减轻尿酸钠所致大鼠关节肿胀度,显示其对痛风性炎症的抗炎作用,其抗炎作用可能与其能降低大鼠关节液TNF-α水平有关。在大鼠肾纤维化模型中,rosmarinic acid能明显抑制大鼠纤维化的形成,其作用强度与阳性对照依那普利相当,其抗纤维化作用可能与其能降低肾小管上皮细胞中TGF-β的表达有关。

关键词： rosmarinic acid acteoside renierol 黄嘌呤氧化酶尿酸高尿酸血症痛风性关节炎肾间质纤维化转化生长因子-β1 肿瘤坏死因子α

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东北鹤虱中具有平滑肌松弛作用和抗菌活性的化学成分研究

东北鹤虱中具有平滑肌松弛作用和抗菌活性的化学成分研究

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作者：张韶瑜天津医科大学

学位级别：硕士

目的:研究民间中药东北鹤虱抗腹泻作用的有效成分,并初步探索其可能的药理活性。方法:以家兔离体回肠平滑肌自律性收缩抑制率为指标,对东北鹤虱的提取物进行药理活性指导性筛选。实验采用硅胶柱层析、凝胶色谱法、和制备HPLC对东... 详细信息

目的:研究民间中药东北鹤虱抗腹泻作用的有效成分,并初步探索其可能的药理活性。方法:以家兔离体回肠平滑肌自律性收缩抑制率为指标,对东北鹤虱的提取物进行药理活性指导性筛选。实验采用硅胶柱层析、凝胶色谱法、和制备HPLC对东北鹤虱的活性部位进行进一步分离纯化。通过各种有机波谱,包括H-NMR,C-NMR,2D-NMR,EI-MS,MS,IR及UV等方法对单体化合物进行结构鉴定,并对单体化合物进行家兔离体回肠平滑肌松弛作用及KB法抗菌作用的研究。结果:东北鹤虱粗分组分及提取物中,高极性部位LEG0118、1119及正丁醇萃取物具有较强的平滑肌松弛活性。从以上三个活性部位分离得到6个单体化合物,其中LEG1402为一新的喹酮类生物碱,鉴定为8-甲氧基-4-喹酮-2羧酸;LEG4801鉴定为rosmarinic acid;LEG3902为一糖苷类化合物,其苷元结构与LEG1402类似;其余化合物的化学结构正在解析过程中。LEG4801和LEG3902具有较强的平滑肌松弛活性,与空白对照组比较Prosmarinic acid为首次从东北鹤虱中分离得到。新化合物8-甲氧基-4-喹酮-2羧酸具有抗菌活性。

关键词：东北鹤虱 rosmarinic acid 喹酮生物碱平滑肌松弛作用抗菌作用

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