De novo biosynthesis of rosmarinic acid in Saccharomyces cerevisiae
作者单位：The SATCM Key Laboratory for New Resources & Quality Evaluation of Chinese MedicineThe MOE Key Laboratory for Standardization of Chinese Medicines and Shanghai Key Laboratory of Compound Chinese MedicinesInstitute of Chinese Materia MedicaShanghai University of Traditional Chinese Medicine Department of Chemical and Biological EngineeringDepartment of Biological SciencesRensselaer Polytechnic Institute
会议名称：《中国化学会第十二届全国天然有机化学学术会议》
会议日期：2018年
学科分类：081703[工学-生物化工] 08[工学] 0817[工学-化学工程与技术] 0836[工学-生物工程] 082203[工学-发酵工程] 0822[工学-轻工技术与工程]
关 键 词：rosmarinic acid Saccharomyces cerevisiae biosynthesis fermentation
摘 要：Rosmarinic acid(RA) and its derivatives like salvianolic acid B are water-soluble phenolic acids original from medicinal plants such as Salvia miltiorrhiza with a variety of pharmacological activities .These compounds have been widely used as medicine and food *** we reported the de novo biosynthesis of RA in engineered Saccharomyces cerevisiae through recreating a six-step minis biosynthetic pathway linked tyrosine to *** recreated pathway containing seven genes devided into two ***,4 CL,HPPR,and RAS consisted the first module and were assembled in plasmid *** coupled with CYP and CPR were the second module and constructed into ***424-TAT-4 CL-HPPR-RAS and pRS426-CYP-CPR-TAL were co-transformed into yeast to do two-step fermentation in synthetic dropout(SD) selection ***-MS results indicated RA was produced at 7.9±0.3 mg/*** work demonstrates that de novo biosynthetic RA pathway has been successfully recreated in *** which sheds light on an alternative source for the biosynthesis of RA and its derivatives such as salvianolic acid B and other phenolic acid compounds.