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Efficient control of root-knot nematodes by expressing Bt nematicidal proteins in root leucoplasts

Molecular Plant 2024年第10期17卷 1504-1519页

作者： Yong Wang Mengnan Wang Yali Zhang Longwei Peng Dadong Dai Fengjuan Zhang Jiang Zhang State Key Laboratory of Biocatalysis and Enzyme Engineering Hubei Hongshan LaboratorySchool of Life SciencesHubei UniversityWuhan 430062China Shenzhen Branch Guangdong Laboratory of Lingnan Modern AgricultureKey Laboratory of Synthetic BiologyMinistry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518000China State Key Laboratory of Agricultural Microbiology College of Life Science and TechnologyHuazhong Agricultural UniversityWuhanChina

root-knot nematodes(RKNs)are plant pests that infect the roots of host *** thuringiensis(Bt)nematicidal proteins exhibited toxicity to ***,the application of nematicidal proteins for plant protection is hampered by the lack of effective delivery systems in transgenic *** this study,we discovered the accumulation of leucoplasts(root plastids)in galls and RKN-induced giant *** infection causes the degradation of leucoplasts into small vesicle-like structures,which are responsible for delivering proteins to RKNs,as observed through confocal microscopy and immunoelectron *** showed that different-sized proteins from leucoplasts could be taken up by Meloidogyne incognita *** further explore the potential applications of leucoplasts,we introduced the Bt crystal protein Cry5Ba2 into tobacco and tomato leucoplasts by fusing it with a transit *** transgenic plants showed significant resistance to ***,RKN females preferentially took up Cry5Ba2 protein when delivered through plastids rather than the *** decrease in progeny was positively correlated with the delivery efficiency of the nematicidal *** conclusion,this study offers new insights into the feeding behavior of RKNs and their ability to ingest leucoplast proteins,and demonstrates that root leucoplasts can be used for delivering nematicidal proteins,thereby offering a promising approach for nematode control.

关键词： root leucoplast Bacillus thuringiensis Cry5Ba2 protein root-knot nematodes pest control

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Determination of Nematicidal Effects of Some Biopesticides against root-knot Nematode （Meloidogyne incognita） on Kiwifruit

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Journal of Agricultural Science and Technology(A) 2017年第8期7卷 546-551页

作者： Ilker Kepenekci Onur Dura Sami Dura Department of Plant Protection Faculty of Agriculture Gaziosmanpasa University Tashciftlik Tokat 60250 Turkey Atatiirk Central Horticultural Research Institute Merkez Yalova 77100 Turkey ROTAM Lifesciences Kimyasal Tartm Tic. Ltd. Sti Pasaport lzmir 35210 Turkey

The aim of this study was to determine the nematicidal effects of some biopesticides againist root-knot nematodes （RKNs）（Meloidogyne incognita） on Bruno type kiwifruit seedlings. Research was conducted in the Atattirk Central Horticultural Research Institute （ACHRI, Yalova, Turkey） greenhouse during 2013-2014. Biopesticides observed in this study were： sesame oil （Devalone EC）, castor oil （Ricinus communis）, Myrothecium verrucaria （M. verrucaria strain AARC-0255, Inferno DF）, Glomus spp. （Endomycorrhizal fungi, Endo roots Soluble SP） and Paecilomyces lilacinus （Entomopathogenic fungi, P. lilacinus strain PL1, Bionematon SL）. Nematicide Nemacur EC 400 （fenamiphos） was used as treated control （TC） and trials were set up as randomized parcel design （RPD） with seven characters and 10 replicates. Reproduction ratio was determined according to scale values in trial applications and second stage juvenile （J2） populations of M. incognita in soil. The results showed that the best nematicidal effects against RKNs were determined in castor oil （77.92% in 2013 and 73.61% in 2014） and P. lilacinus （61.03% in 2013 and 55.55% in 2014）.

关键词： root-knot nematodes Meloidogyne incognita sesame oil castor oil Myrothecium verrucaria Glomus Paecilomyceslilacinus kiwifruit.

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Comparing Immunity Triggered by flg22 Derived from Nonpathogenic Pseudomonas fragi Sneb1990 and Pathogenic P. syrinage pv. tomato DC3000

Comparing Immunity Triggered by flg22 Derived from Nonpathog...

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中国植物病理学会2024年学术年会

作者： ZHAO Shuangling ZHU Xiaofeng FAM Haiyan LIU Xiaoyu WANG Yuanyuan YANG Ning CHEN Lijie DUAN Yuxi College of Plant Protection Shenyang Agricultural University Nematology Institute of Northern China Shenyang Agricultural University College of Sciences Shenyang Agricultural University College of Biological Science and Technology Shenyang Agricultural University

Tomato（Solanum lycopersicum L.） is one of the most economically valuable vegetables for both the fresh and the processed vegetable ***-parasiticnematodes disease,caused by root-knot nematodes（Meloidogyne spp.）,has become endemic in many green-house and caused tremendous economic losses to the vegetable *** have a multilayer immune system to defend themselves against pathogen ***,it is of vital importance to investigate the biocontrol bacteria that stimulate the plant immune system to help improve the resistance of tomato to root-knot nematode *** previous results showed that biocontrol bacteria Pseudomonas fragi Sneb1990 could trigger defense-related gene expression changes in tomato,and the amount of nematode infestation after treatment with flg22 was significantly less than that of *** this study,comparison of the defense response of tomato to the flg22 derived from *** Sneb1990 and pathogenicPst DC3000,was performed by analyzing the expression of several defense-associated *** root tissue from root dip treatment with flg22,the expression of canonical defense-related genes was *** data suggested that flg22 specifically induced the expression of certain defense-associated genes in Solanum lycopersicum L.L402 tomato as early as 6 h,and up to 24 h（PR2,NPR1 and PR4） after *** only gene that did not show a significant expression in the first 72 h was *** the case of lignin synthesis related genes expression level,we observed that higher expression levels of PAL（by 5.2-fold） after flg22 treatment compared to the positive controls flg22 and the water *** experiment results may reveal the mechanism by which tomato rapidly responds to the flg22-triggered response and modulates plant immunity to reduce root-knot nematode infestation.

关键词： Pseudomonas fragi Sneb 1990 flg22 plant immunity root-knot nematodes

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