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Effect of Riboflavin deficiency on intestinal morphology,jejunum mucosa proteomics,and cecal microbiota of Pekin ducks

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Animal Nutrition 2023年第1期12卷 215-226页

作者： Yaxi Xu Bo Zhang Rui Zhao Kexin Gao Suyun Liang Yongbao Wu Yongsheng Hao Dapeng Liu Zhanbao Guo Jian Hu Zhengkui Zhou Ming Xie Jing Tang State Key Laboratory of Animal Nutrition Key Laboratory of Animal(Poultry)Genetics Breeding and ReproductionMinistry of Agriculture and Rural AffairsInstitute of Animal ScienceChinese Academy of Agricultural SciencesBeijing100193China

This study was to determine the effects of Riboflavin deficiency(RD)on intestinal development,jejunum mucosa proteome,cecal short-chain fatty acids(SCFA)profiling,and cecal microbial diversity and community of starter Pekin ducks.Male white Pekin ducks(1 d old,n=240)were allocated into 2 groups,with 12 replicates and 10 birds per replicate in each group.For 21 d,all ducks had ad libitum access to either an RD or a Riboflavin adequate(control,CON)diet,formulated by supplementing a basal diet with 0 or 10 mg Riboflavin per kg of diet,respectively.Compared to the CON group,growth retardation,high mortality,and poor Riboflavin status were observed in the RD group.Furthermore,RD reduced the villus height and the ratio of villus height to crypt depth of jejunum and ileum(P<0.05),indicating morphological alterations of the small intestine.In addition,dietary RD enhanced relative cecum weight and decreased cecal SCFA concentrations(P<0.05),including propionate,isobutyrate,butyrate,and isovalerate.The jejunum mucosa proteomics showed that 208 proteins were upregulated and 229 proteins were downregulated in the RD group compared to those in the CON group.Among these,RD mainly suppressed intestinal absorption and energy generation processes such as glycolysis and gluconeogenesis,fatty acid beta oxidation,tricarboxylic acid cycle,and oxidative phosphorylation,leading to impaired ATP generation.In addition,RD decreased the community richness and diversity of the bacterial community in the cecum of ducks.Specifically,RD reduced the abundance of butyrate-producing bacteria in the cecum(P<0.05),such as Eubacterium coprostanoligenes,Prevotella and Faecalibacterium.Dietary RD resulted in growth depression and intestinal hypofunction of Pekin ducks,which could be associated with impaired intestinal absorption and energy generation processes in intestinal mucosa,as well as gut microbiota dysbiosis.These findings contribute to our understanding of the mechanisms of intestinal hypofunction due to RD.

关键词： Riboflavin Deficiency Intestinal morphology Proteomics Cecal microbiota Short-chain fatty acids

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Cell-penetrating Riboflavin conjugate for antitumor photodynamic therapy

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Chinese Chemical Letters 2022年第9期33卷 4339-4344页

作者： Chunlei Wu Yanyan Li Zhehong Cheng Pengxin Wang Zhilong Ma Ke Liu Yulian Cheng Yimin Zhou Xian Lin Ximing Shao Yong Yang Hongchang Li Lijing Fang Guangdong Key Laboratory of Nanomedicine Institute of Biomedicine and BiotechnologyShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China University of Chinese Academy of Sciences Beijing 100049China Department of Biomedical Engineering Southern University of Science and Technology(SUSTech)Shenzhen 518055China

Riboflavin(RF,vitamin B2)is an essential vitamin and has been considered as a promising natural photosensitizer for photodynamic therapy(PDT).However,further exploration of RF in antitumor application was limited by its poor cellular uptake.In this study,using cell-penetrating peptides Arg8,(Cha-Arg)3 and small molecule triphenylphosphine(TPP)as delivery compounds,three RF conjugates were prepared to increase the accumulation of RF in cells,termed as Arg8-RF,(Cha-Arg)3-RF and TPP-RF,respectively.Compared with TPP-RF and Arg8-RF,(Cha-Arg)3-RF exhibited better cell internalization and stronger cytotoxicity against HeLa cells upon exposure to blue light.Further researches proved that(Cha-Arg)3-RF generated reactive oxygen species(ROS)under irradiation,which could indiscriminately destroy endogenous proteins and mitochondria,ultimately inducing cell death.This work provides a new approach to explore RF as a natural photosensitizer for antitumor photodynamic therapy.

关键词： Riboflavin Antitumor Photodynamic therapy Cell-penetrating peptides

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Synthesis of highly fluorescent carbon dots from lemon and onion juices for determination of Riboflavin in multivitamin/mineral supplements

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Journal of Pharmaceutical Analysis 2019年第3期9卷 209-216页

作者： Severino S. Monte-Filho Stefani I.E. Andrade Marcelo B. Lima Mario C.U. Araujo Universidade Federal da Paraíba CCEN Departamento de Química P.O. Box 5093 Zip Code 58051-970 Joao Pessoa PB Brazil Universidade Federal Rural de Pernambuco-Unidade Acad^emica de Serra Talhada UAST Zip Code 56909-535 Serra Talhada PE Brazil

In this work, lemon and onion biomasses commonly found in street markets are for the first time used to develop a facile, fast and low-cost one-step microwave-assisted carbonization method for synthesis of highly fluorescent carbon dots (CDs). The structure and optical properties of CDs were investigated by TEM, XRD, XRF, UV-Vis, FTIR, and fluorescence spectroscopy. CDs displayed satisfactory optical pro-prieties, a high quantum yield of 23.6%, and excellent water solubility, and the particle size was 4.23-8.22 nm with an average diameter of 6.15 nm. An efficient fluorescent resonance energy transfer (FRET) between the CDs and Riboflavin was achieved with CDs acting as donor and Riboflavin as acceptor. A linear relationship between FRET and the Riboflavin concentration from 0.10 to 3.0 μg/mL was observed, allowing the development of an accurate and fast analytical method to determine this vitamin in multivitamin/mineral supplements. Despite the potential interferences in these supplements, CDs were selective for Riboflavin under optimized conditions. A paired t-test at a 95% confidence level indicated no statistically significant difference between the proposed and the reference methods. Recovery test presented values ranged from 96.0% to 101.4%. The limit of detection and relative standard deviation were estimated at 1.0 ng/mL and Riboflavin.

关键词： Carbon dots Microwave-assisted carbonization Lemon-onion juice Riboflavin Multivitamin/mineral supplements Fluorescence

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Sociodemographic Factors Associated with Dietary Intake of Thiamine, Riboflavin, and Niacin among Chinese Adults in 2015

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Biomedical and Environmental Sciences 2020年第9期33卷 660-669页

作者： LI Li ZHANG Bing WANG HuiJun OUYANG Yi Fei HUANG Fei Fei WANG Yun ZHANG Ji Guo SU Chang DU Wen Wen JIA Xiao Fang JIANG Hong Ru WANG Zhi Hong National Institute for Nutrition and Health Chinese Center for Disease Control and PreventionBeijing 100050China

Objective To estimate the association between three B-vitamin intakes and sociodemographic factors among adults in China.Methods We derived our data from the China Health and Nutrition Survey(CHNS) among 12,241 individuals aged 18–64 years. Log binomial regression was used to estimate adjusted prevalence ratios for factors associated with the inadequate intake of B-vitamins.Results Females with low incomes and living in the north had a higher prevalence of inadequate Riboflavin intake than those with high incomes and living in the south. Both males and females living in a village had a higher prevalence of inadequate Riboflavin intake than adults living in a city. Adults with low income, low education, and living in the north or in a village had a higher prevalence of inadequate niacin intake than adults with a high income, high education, and living in the south or in a city.Conclusion We found that income, region, and area of residence were associated with Riboflavin intake. Education, income, region, and area of residence were associated with niacin intake. Welltailored strategies and policies are needed to improve nutritional status in China.

关键词： Thiamine Riboflavin Niacin Vitamin B deficiency Nutritional requirements China

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Iontophoresis-assisted corneal crosslinking using 0.1% Riboflavin for progressive keratoconus

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International Journal of Ophthalmology(English edition) 2017年第5期10卷 717-722页

作者： Hong-Zhen Jia Xu Pang Zheng-Jun Fan Na Li Gang Li Xiu-Jun Peng Chinese PLA Medical School Beijing 100853 China Department of Ophthalmology Chinese PLA Navy General Hospital Beijing 100048 China

AIM：To report the clinical results of iontophoresis-assisted epithelium-on corneal crosslinking（I-CXL） using 0.1% Riboflavin in distilled water for progressive keratoconus. METHODS：In this prospective clinical study, we examined 94 eyes of 75 patients with progressive keratoconus who were treated with I-CXL using 0.1% Riboflavin in distilled water. Best correct visual acuity（BCVA）, Scheimpflug tomography, corneal topography, anterior segment optical coherence tomography, intraocular pressure, and endothelial cell density were evaluated at baseline and 1, 3, 6, 12, and 24 mo after I-CXL.RESULTS：After 24 mo I-CXL, compared to the level at baseline, BCVA significantly improved 0.14±0.07（P=0.010）; mean keratometry signifi cantly decreased 0.72±1.97（P=0.021）; maximum keratometry significantly reduced 2.30±5.01（P=0.014）; central keratoconus index significantly reduced 0.04±0.08（P=0.007）. The demarcation line was visible in 83.1% of eyes at 1mo after treatment, with a depth of 298.95±51.97 μm, and gradually indistinguishable. One eye had repeat treatment. Intraocular pressure and endothelial cell density did not change significantly.CONCLUSION：I-CXL using 0.1% Riboflavin halts keratoconus progression within 24 mo, resulting in a significant improvement in visual and topographic parameters. Moreover, the depth of the demarcation line is similar to that previously reported in standard epithelium-off CXL procedures.

关键词： corneal cross-linking iontophoresis keratoconus distilled water Riboflavin

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A Historical Cohort Study on the Efficacy of Glucocorticoids and Riboflavin Among Patients with Late-onset Multiple AcyI-CoA Dehydrogenase Deficiency

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Chinese Medical Journal 2016年第2期129卷 142-146页

作者： Xin-Yi Liu Zhi-Qiang Wang Dan-Ni Wang Min-Ting Lin Ning Wang Department of Neurology and Institute of Neurology First Affiliated Hospital Fujian Medical University Fuzhou Fujian 350005 China Fujian Key Laboratory of Molecular Neurology Fuzhou Fujian 350005 China

Background： Late-onset multiple acyl-CoA dehydrogenase deficiency （MADD） is the most common type of lipid storage myopathies in China. Most patients with late-onset MADD are well responsive to Riboflavin. Up to now, these patients are often treated with glucocorticoids as the first-line drug because they are misdiagnosed as polymyositis without muscle biopsy or gene analysis. Although glucocorticoids seem to improve the fatty acid metabolism of late-onset MADD, the objective evaluation of their rationalization on this disorder and comparison with Riboflavin treatment are unknown. Methods： We performed a historical cohort study on the efficacy of the two drugs among 45 patients with late-onset MADD, who were divided into glucocorticoids group and Riboflavin group. Detailed clinical information of baseline and 1-month follow-up were collected. Results： After 1-month treatment, a dramatic improvement of muscle strength was found in Riboflavin group （P 〈 0.05）. There was no significant difference in muscle enzymes between the two groups. Significantly, the number of patients with full recovery in glucocorticoids group was less than the number in Riboflavin group （P 〈 0.05）. On the other hand, almost half of the patients in Riboflavin group still presented high-level muscle enzymes and weak muscle strength after 1-month Riboflavin treatment, meaning that l-month treatment duration maybe insufficient and patients should keep on Riboflavin supplement for a longer time. Conclusions： Our results provide credible evidences that the overall efficacy of Riboflavin is superior to glucocorticoids, and a longer duration of Riboflavin treatment is necessary for patients with late-onset MADD.

关键词： Glucocorticoids Historical Cohort Study Late-onset Multiple Acyl-CoA Dehydrogenase Deficiency Lipid StorageMyopathy Riboflavin

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Riboflavin concentration in corneal stroma after intracameral injection

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International Journal of Ophthalmology(English edition) 2015年第3期8卷 470-475页

作者： Na Li Xiu-Jun Peng Zheng-Jun Fan Xu Pang Yu Xia Teng-Fei Wu Chinese PLA Medical School & Chinese PLA General Hospital Department of Ophthalmology Navy General Hospital of Chinese PLA

AIM: To evaluate the enrichment of Riboflavin in the corneal stroma after intracameral injection to research the barrier ability of the corneal endothelium to Riboflavin in vivo.METHODS: The right eyes of 30 New Zealand white rabbits were divided into three groups. Different concentrations Riboflavin-balanced salt solutions(BSS)were injected into the anterior chamber(10 with 0.5%, 10 with 1%, and 10 with 2%). Eight corneal buttons of 8.5mm in diameter from each group were dissected at 30 min after injection and the Riboflavin concentrations in the corneal stroma were determined using high-performance liquid chromatography(HPLC) after removing the epithelium and endothelium. The other two rabbits in every group were observed for 24 h and sacrificed. As a comparison, the Riboflavin concentrations from 16 corneal stromal samples were determined using HPLC after instillation of 0.1% Riboflavin-BSS solution for30 min on the corneal surface(8 without epithelium and 8with intact epithelium).RESULTS: The mean Riboflavin concentrations were11.19, 18.97, 25.08, 20.18, and 1.13 μg/g for 0.5%, 1%, 2%,de-epithelialzed samples, and the transepithelial groups,respectively. The color change of the corneal stroma and the HPLC results showed that enrichment with Riboflavin similar to classical de-epithelialized corneal collagen crosslinking(CXL) could be achieved by intracameral 1%Riboflavin-BSS solution after 30min; the effect appeared to be continuous for at least 30 min.CONCLUSION: Riboflavin can effectively penetrate the corneal stroma through the endothelium after an intracameral injection in vivo, so it could be an enhancing method that could improve the corneal Riboflavin concentration in transepithelial CXL.

关键词： Riboflavin cornea intracameral injection high-performance liquid chromatography endothelium

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Evaluation of the in vitro antimicrobial properties of ultraviolet A/Riboflavin mediated crosslinking on Candida albicans and Fusarium solani

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International Journal of Ophthalmology(English edition) 2014年第2期7卷 205-210页

作者： Bing Sun Zhi-Wei Li Hai-Qun Yu Xiang-Chen Tao Yong Zhang Guo-Ying Mu Department of Ophthalmology Provincial Hospital Affiliated to Shandong University Department of Ophthalmology Provincial Hospital Affiliated to Anhui Medical University Department of Ophthalmology Affiliated Hospital of Weifang Medical University

AIMTo evaluate the antimicrobial properties of ultraviolet A (UVA) (365 nm)/Riboflavin against Candida albicans and Fusarium solani.

关键词： antimicrobial crosslinking fungal Riboflavin ultraviolet A

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Effects of nicotinamide and Riboflavin on the biodesulfurization activity of dibenzothiophene by Rhodococcus erythropolis USTB-03

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Journal of Environmental Sciences 2008年第5期20卷 613-618页

作者： YAN Hai SUN Xudong XU Qianqian MA Zhao XIAO Chengbin Ning Jun Department of Biological Science and Technology School of Applied Science University of Science and Technology Beijing Beijing 100083 China Research Center for Eco-environmental Science Chinese Academy of Sciences Beijing 100085 China

Rhodococcus erythropolis USTB-03 is a promising bacterial strain for the biodesulfurization of dibenzothiophene （DBT） via a sulfurspecific pathway in which DBT is converted to 2-hydroxybiphenyl （2HBP） as an end product. The effects of nicotinamide and Riboflavin on the sulfur specific activity （SA） of DBT biodesulfurization by R. erythropolis USTB-03 were investigated. Both nicotinamide and Riboflavin were found to enhance the expression of SA, which was not previously reported. When R. erythropolis USTB-03 was grown on a medium containing nicotinamide of 10.0 mmol or Riboflavin of 50.0 μmol, SA was raised from 68.0 or so to more than 130 mmol 2HBP/（kg dry cells.h）. When R. erythropolis USTB-03 was grown in the presence of both nicotinamide of 5.0 mmol and Riboflavin of 25.0 μmol, SA was further increased to 159.0 mmol 2HBP/（kg dry cells.h）. It is suggested that the biological synthesis of reduced form of flavin mononucleotide （FMNH2）, an essential coenzyme for the activities of biodesulfurization enzyme Dsz C and A, might be enhanced by nicotinamide and Riboflavin, which was responsible for the increased SA of R. erythropolis USTB-03.

关键词： biodesulfurization dibenzothiophene nicotinamide Rhodococcus erythropolis USTB-03 Riboflavin

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Photosensitized damage to telomere overhang and telomerase RNA by Riboflavin

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Progress in Natural Science:Materials International 2008年第4期18卷 401-404页

作者： Yuxia Liu, Fuqiang Du, Weizhen Lin , Tiecheng Tu, Wenxin Li, Nianyun Lin Key Laboratory of Nuclear Analysis Techniques, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China Key Laboratory of Nuclear Analysis Techniques Shanghai Institute of Applied Physics Chinese Academy of Sciences Shanghai 201800 China

By ESR spin elimination and photocleavage assay, the mechanisms of one-electron oxidation damage of oligonucleotides by excited triplet state of Riboflavin (Rb) have been elucidated. The results demonstrate that Rb, an endogenous photosensitizer, is capable of cleav- ing single-stranded telomeric overhang and the template region of telomerase RNA under UVA irradiation, resulting in blocking of reverse transcription of telomeric DNA which leads to the apoptosis of cancer cells ultimately.

关键词： Telomere Telomerase Riboflavin ESR spin elimination Excited triplet state

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