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Researches of Agrobacterium rhizogenes ri plasmid rol Genes

Forestry Studies in China 2002年第1期4卷 58-64页

作者：梁机林善枝郭海陈晓阳北京林业大学生物科学与技术学院北京100083

Expression of rol genes from ri plasmid of Agrobacterium rhizogenes not only leads to the excessive formation of adventitious roots, but also exhibits various genetically modified characteristics that have broad prospects for the application of plant genetic improvement. Since the 1980s of the last century, much progress has been made in the studies of A. rhizogenes, in particular the agropine type ri plasmid rol genes and their applications for plant genetic improvement, which involves the structure and function of ri plasmid, the characters of rol genes, the influence of rol genes expression on plants growth and development, and the applications of rol genes for genetic improvement of forest tree. In this paper, the advances in this field are reviewed and the existing problems about the application of rol genes for genetic improvement of forest tree are also discussed.

关键词： Agrobacterium rhizogenes ri plasmid rol genes phenotypic alterations genetic improvement of forest tree

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Hairy Root and Its Application in Plant Genetic Engineering

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Journal of Integrative Plant Biology 2006年第2期48卷 121-127页

作者： Zhi-Bi Hu Min Du Institute of Chinese Materia Medica Shanghai University of Traditional Chinese Medicine Shanghai 201203 China

Agrobacterium rhizogenes Conn. causes hairy root disease In plants. Hairy root-Infected A. rhizogenes Is characterlzed by a high growth rate and genetic stability. Hairy root cultures have been proven to be an efficient means of producing secondary metabolites that are normally biosyntheslzed In roots of differentiated plants. Furthermore, a transgenlc root system offers tremendous potential for introducing additional genes along with the ri plasmld, especially with modified genes, into medicinal plant cells with A. rhizogenes vector systems. The cultures have turned out to be a valuable tool with which to study the biochemical properties and the gene expression profile of metabolic pathways. Moreover, the cultures can be used to elucidate the Intermediates and key enzymes Involved In the biosynthesis of secondary metabolites. The present article discusses various appllcations of hairy root cultures in plant genetic engineering and potential problems aseoclsted with them.

关键词： Agrobacterium rhizogenes hairy root plant genetic engineering ri plasmid secondary metabolites.

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