Hairy Root and Its Application in Plant Genetic Engineering

作     者：Zhi-Bi Hu Min Du 

作者机构：Institute of Chinese Materia Medica Shanghai University of Traditional Chinese Medicine Shanghai 201203 China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2006年第48卷第2期

页      面：121-127页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

基　　金：Supported by the National Natural Science Foundation of China (30100237) 

主　　题：Agrobacterium rhizogenes hairy root plant genetic engineering Ri plasmid secondary metabolites. 

摘      要：Agrobacterium rhizogenes Conn. causes hairy root disease In plants. Hairy root-Infected A. rhizogenes Is characterlzed by a high growth rate and genetic stability. Hairy root cultures have been proven to be an efficient means of producing secondary metabolites that are normally biosyntheslzed In roots of differentiated plants. Furthermore, a transgenlc root system offers tremendous potential for introducing additional genes along with the RI plasmld, especially with modified genes, into medicinal plant cells with A. rhizogenes vector systems. The cultures have turned out to be a valuable tool with which to study the biochemical properties and the gene expression profile of metabolic pathways. Moreover, the cultures can be used to elucidate the Intermediates and key enzymes Involved In the biosynthesis of secondary metabolites. The present article discusses various appllcations of hairy root cultures in plant genetic engineering and potential problems aseoclsted with them.